Insect resistance to Bt toxin is related with the variation of cadherin-like protein gene.cDNA fragment of cadherin-like protein gene in the fifth instar larva of oriental tobacco budworm was amplified using degeneracy primers and RT-PCR technique,and the target fragment was further sequenced after being cloned into pMD18-T vector plasmid.

本研究以煙夜蛾五齡幼蟲中腸的總RNA為模板干天天干，根據(jù)已經(jīng)克隆的其他昆蟲的類鈣粘蛋白基因序列設計引物久久精品视频无，利用RT-PCR技術擴增出了煙夜蛾類鈣粘蛋白基因的cDNA片段黄色网站毛片，將其連接到pMD18-T載體理伦无码在线观看，轉(zhuǎn)化大腸桿菌后篩選陽性克隆并進行序列測定cao欧美色就是色，測序得到的1 335 bp的片斷編碼444個氨基酸殘基偷国产乱人伦精品一区二区。

With primers designed according to sequence(AY052417) of diapause pupae of oriental tobacco budworm registered on GenBank, a double-strand cDNA fragment of DH gene was obtained from RT-PCR amplification and was further cloned into pGEM-T Easy vector.

根據(jù)GenBank發(fā)表序列(AY052417)設計引物，以煙夜蛾(Helicoverpa assulta Guenée)滯育蛹為材料尤物网视频在线，利用RT-PCR技術擴增到滯育激素(diapause hormone欧美a片网止，DH)cDNA，并通過TA克隆的方法將其克隆至pGEM-T Easy載體上高清在线看欧美亚洲中文字幕，對目的基因進行測序中文字幕乱码一区二区久久，并利用設計的酶切位點將目的基因從T載體上切下。

There is neither paper about detecting DsMV by DBH or IC-RT-PCR nor report about comparing ELISA with these methods published before. Therefore, the DsMV detection methods developed in this study can be applied to virus certification scheme of calla lily and help to produce healthy seedlings in Taiwan.

之前未曾有以DBH或IC-RT-PCR檢測DsMV之報告做a视频免费在线观看，或這些方法與ELISA比較的相關報導一级黄片美女的奶水，因此希望本研究所研發(fā)的DsMV檢測方法未來可應用於每芋種苗病毒驗證作業(yè)，以協(xié)助國內(nèi)業(yè)者生產(chǎn)健康海芋種苗无码一级午夜福利2021。

METHODS:The expression of Ki67 in three breast caner cell lines was detected by half-quantitative RT-PCR and Western blot assay.

采用半定量RT-PCR和Western blot分別檢測3株乳腺癌細胞中Ki67mRNA和蛋白表達水平国产综合精品久久亚洲。

The effect of carotenoid on the bcl-2 gene expression of breast cancer cell lines MCF-7 cell was determined by the method of RT-PCR.

采用RT-PCR方法檢測類胡蘿卜素對MCF-7細胞株的Bcl-2基因表達的影響，均呈不同程度的下調(diào)www的网址免费看。

Methods The target DNA sequence of yrdC was obtained from human spleen tissue by using RT-PCR,construct high titer recombinant adenovirus Ad-yrdC by using AdEasy adenovirus carrier system,detect yrdC protein expression by using Western blot and immunohistochemical method,set up control group and transfected Ad-Null group,and study the effects Ad-yrdC on BGC-823 cell line by drawing cell growth curve and MTT chromatometry.

方法用RT-PCR方法獲取人脾臟組織yrdC基因序列黄色视频一级片黄色，采用AdEasy腺病毒載體系統(tǒng)構(gòu)建攜帶yrdC基因的高滴度重組腺病毒Ad-yrdC，將其轉(zhuǎn)染胃癌BGC-823細胞欧美一级做a影片爱橙影院，應用Western blot和免疫組織化學法檢測yrdC蛋白表達网友自拍第1页，設對照組和轉(zhuǎn)染Ad-Null組，并分別繪制細胞生長曲線和MTT比色法研究Ad-yrdC對BGC-823細胞的影響。

The chondrocytes of different generation were observed with light-microscope and transmission electron microscope for cellular growth and ultromicrostructure, with the method of MTT assay for grow curve and proliferation, with alcian blue test for GAG of ECM, with immuocytochemistry and RT-PCR (reverse transcript -polymerase chain reaction)for type Ⅱcollagen, with flow cytometry for cell life cycle ,histochemistry for S-A-β-galand so on by which to identify cataplasia and senescence of chondrocytes cultured in vitro.

對軟骨細胞退變老化進行觀察欧美日韩三级片，采用相差顯微鏡觀察其生長情況极黄网站，透射電鏡觀察細胞結(jié)構(gòu)，阿力新藍染色檢測胞外基質(zhì)硫酸GAG含量和結(jié)構(gòu)玖玖伊人，免疫細胞化學法和RT-PCR方法檢測Ⅱ型膠原鑒定軟骨細胞国产一级婬片!A片AAA毛片男男?，以MTT比色法描繪生長曲線、檢測生長狀態(tài)A级毛片观看免费，組化法檢測老化相關β-半乳糖苷酶国产一区二区狠干，流式細胞儀分析細胞周期和增殖指數(shù)。3欧美黑人粗暴交的一级视频。

The third passage chondrocytes were divided into blank group, different desity PAP groups, different desity glucosaminsalfate groups which were passaged to 4th generation and contrast to the 2nd passage group. The chondrocytes of different groups were detected with the method of histochemistry for S-A-β-gal,and with alcian blue test for the content and constructure of GAG of ECM, immuocytochemistry for type Ⅱcollagen and PCNA, MTT assay for proliferation, RT-PCR for type Ⅱcollagen and Aggrecan, flow cytometry for cell life cycle and proliferation index,by which to observe PAP's function regarding to the appearance and functional status in the process of chondrocyte's cataplasia and senescence.

將P3軟骨細胞分為空白對照組一级欧美牲交片无遮、鹿茸多肽不同濃度組、硫酸氨基葡萄糖不同濃度組進行傳代培養(yǎng)狠狠色色欲，同時以P2代軟骨細胞為對照組小黄片毛片在线，進行組化檢測老化相關β-半乳糖苷酶，阿力新藍染色檢測胞外基質(zhì)硫酸GAG含量和結(jié)構(gòu)与子的性关系视频，MTT比色檢測增殖爱搞免费，免疫細胞化檢測PCNA和Ⅱ型膠原，RT-PCR檢測Ⅱ型膠原天天做天天爱天天爽天天综合、Aggrecan蛋白久久影院A片AAA毛片，流式細胞儀分析細胞周期和增殖指數(shù)等方法，對鹿茸多肽抗軟骨細胞退變老化進行分子生物學研究毛片基地在线视频无码。4国产精品久久久久影院老司。

The 3rdpassage chondrocytes were divided into blank group, different concentration PAP groups,different concentration glucosaminsalfate groups and were sequently passaged to 4thgeneration. The 2nd passage chondrocytes was contrasted as young cells group. Thechondrocytes of different groups were detected with the methods of histochemistry forS-A-β-gal, and with alcian blue test for the content and constructure of GAG of ECM,immuocytochemistry for typeⅡcollagen and PCNA, MTT assay for proliferation, RT-PCRfor typeⅡcollagen and Aggrecan, flow cytometry for cell life cycle and proliferationindex,by which to observe PAP"s function regarding to the appearance and functional status inthe process of chondrocyte"s cataplasia and senescence.

將P3軟骨細胞分為空白對照組、鹿茸多肽不同濃度組欧美亚性爱小说视频一二三四、硫酸氨基葡萄糖不同濃度組進行傳代培養(yǎng)人人摸日操，同時以P2代軟骨細胞為對照組，進行組化檢測老化相關β-半乳糖苷酶日韩欧美一本，阿力新藍染色檢測胞外基質(zhì)硫酸GAG含量和結(jié)構(gòu)99熟妇，MTT比色檢測增殖，免疫細胞化檢測PCNA和Ⅱ型膠原91精品丝袜在线播放，RT-PCR檢測Ⅱ型膠原18岁黄色网站在线观看、Aggrecan蛋白，流式細胞儀分析細胞周期和增殖指數(shù)等方法亚洲九月小视频，對鹿茸多肽抗軟骨細胞退變老化進行分子生物學研究在线无码午夜福利高潮视频。4。

Total RNA was extracted from flowers of cattleya, according to the conserved acid sequence for ACC oxidase in other orchids, we designed a pair of oligo nucleotide primers. Using RT-PCR method, a cDNA fragment about 967 base pair, which encoded 321 predicted amino acid residues, was amplified.

以卡特蘭花瓣為試材一级做a爱过程免费视，提取其總RNA国产主播第2页，并根據(jù)其他蘭花的ACC氧化酶(1-aminocyclopropane-1-carboxylic acid oxidase一区黄色在线免费观看， ACO)基因保守序列設計一對特異性引物，通過RT-PCR法克隆得到1條967bp的卡特蘭ACO cDNA片斷日本中文字幕网站，共編碼321個氨基酸殘基在线毛片.com。