Using Cassia tora as material,a cDNA encoding chalone synthase was cloned from tender leaves of Cassia tora by RT-PCR and RACE.The full-length cDNA of CHS from Cassia tora had 1 459 bp with an open reading frame encoding 390 amino acids of protein.

以決明為實(shí)驗(yàn)材料加勒比首页，利用RT-PCR和RACE技術(shù)久久本道久久综合伊人，從決明嫩葉中克隆出查爾酮合成酶(Chal-one synthase女老师高潮喷水，CHS)基因色呦呦一站二站，其cDNA全長(zhǎng)為1 459 bp，編碼一個(gè)由390個(gè)氨基酸殘基組成的多肽A级毛片视频。

Main methods used in this study include immunohistochemistry, Laser Confocal Scanning Microscope , cell ELISA, RT-PCR and cell chemotaxis.

實(shí)驗(yàn)方法主要包括：免疫組化少妇AA毛片在线播放、激光共聚焦、細(xì)胞ELISA日本熟女强奸乱码、RT-PCR和細(xì)胞趨化實(shí)驗(yàn)等国产情侣免费在线视频。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究過(guò)程中，采用了病毒接種技術(shù)99热这里只有精品久久、普通病理學(xué)研究方法桃花视频日本高清视频、流式細(xì)胞儀技術(shù)、RT-PCR技術(shù)九色自拍视频在线观看、原位雜交技術(shù)不卡a片免费在线观看、免疫組化技術(shù)、凋亡細(xì)胞末端標(biāo)記技術(shù)www日韩网站，系統(tǒng)研究了不同日齡的SPF雛雞人工感染AEV-NH937株后的病理變化一级a性色生活片毛片黄长官一，T和B淋巴細(xì)胞、T淋巴細(xì)胞亞群的動(dòng)態(tài)變化規(guī)律国产美女特级片免费视频播放器，淋巴細(xì)胞表面某些粘附分子的變化可以直接看黄色视频的网站。，淋巴細(xì)胞和神經(jīng)元的壞死與凋亡，雛雞腦組織中產(chǎn)生趨化因子MIP-1β和IL-8細(xì)胞的變化規(guī)律，腦組織中圍官性細(xì)胞浸潤(rùn)的細(xì)胞類型欧美日韩精品二区在线。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

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Semi-quantitative RT-PCR analysis showed that ZF1 expressed in root, stem, leaf, flower, immature pod, and embryo of chickpea with different expression patterns.

半定量RT-PCR分析表明，ZF1在鷹嘴豆的根超碰免费欧美自拍、莖91av色片在线、葉、花www色色、幼莢和幼胚中均有表達(dá)亚洲第一区色色色，在莖和葉中表達(dá)較弱，為組成型轉(zhuǎn)錄因子欧美性爱最黄第一视频区。

Methods: We extract hippocampus chondriosome from the vascular dementia rat and measure the activity of mitochondrial COX by the UV-spectrophotometer, then detect its gene order through RT-PCR.

從VD大鼠中提取海馬線粒體利用紫外分光光度計(jì)技術(shù)檢測(cè)各組大鼠海馬COX活性一级AAA毛片无码视频，通過(guò)RT-PCR等技術(shù)對(duì)其基因序列進(jìn)行檢測(cè)。

PCR, RT-PCT and PCR-Southern blot analysis indicated that phyB gene was successfully integrated into citrange genome and was transcribed in citrange.

PCRAV免费网站A片、RT-PCR和PCR Southern雜交結(jié)果表明：phyB基因已整合到枳橙的基因組欧美性爱大屁股，并得到了轉(zhuǎn)錄；轉(zhuǎn)phyB基因枳橙表現(xiàn)出植株矮化国内精品1024地址一地址二、分枝角度加大欧美日韩精品综合、葉片變小、比葉干重增加琪琪电影网午夜理论片717百度，其葉綠素含量比對(duì)照高29.1％全网国产黄色视频。

The mRNA of pituitary glands from seven important economic Pleuronectiformes fishes was extracted,the cDNA sequences of GH gene were then isolated from Cleisthenes herzensteini,Limanda yokohamae,Pleuronichthys Cornutus,Cynoglossus robustus,Platichthys bicoloratus, Zebrias zebra and Paralichthys dentatus with RT-PCR method by using designed specific primers based on the reported GH cDNA sequences of Pleuronectiformes. The results of sequencing showed that lengths of the above cDNAs are as follows: 479 bp,564 bp,519 bp,440 bp,564 bp,440 bp and 522 bp.The GH cDNA sequences encode mature polypeptide of about 140 to 170 amino acid.The full-length growth hormone cDNAs of Paralichthys lethostigma and Scophthalmus maximus were cloned by Switching Mechanism At 5\' end of the RNA Transcript RACE technology.

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For the time being,the NDV detection of CIQ includes cloaca tampon separate virus,ELISA,HI and RT-PCR is trying now.

目前我國(guó)出入境檢疫中對(duì)新城疫病毒的檢測(cè)方法主要有瀉殖腔棉拭子樣品病毒分離鑒定亚洲无码操B视频、HI和ELISA日韩综合小视频，RT-PCR檢測(cè)技術(shù)正在試用之中。

In addition, using RT-PCR to detect expression of centrin homogene during Urechis unicinctus spermatid deferentiation and early embryonic development. The result revealed that (1) expression of HsCEN1 homogene is present during spermatid defferentiation including spermtid and sperm, but not in adult tissues including body wall tissue,intestinal mucous memberane cells and cell in coelom succus;(2) expression of HsCEN1 homogene is not present in unfertilized oocytes but present in that of released polar bodies after fertilization and parthenogenesis oocytes treated with A23187;(3) expression of HsCEN1 homogene is present during early embryonic development including two-cell stage,multicell stage,gastrula to trochophora.

我們還用RT-PCR方法檢測(cè)了Centrin同源基因在單環(huán)刺螠精子分化過(guò)程和早期胚胎發(fā)育過(guò)程的表達(dá)18禁肉肉无遮挡无码网站公司，結(jié)果顯示：在單環(huán)刺螠精子發(fā)生過(guò)程中的精細(xì)胞爽爽爽午夜福利试看、精子團(tuán)、精子中都有表達(dá)5060av，而在成體的體壁組織色哟呦在线播放、消化道組織、體腔球中則沒(méi)有表達(dá)国产二区三区毛片大全；在未受精卵中沒(méi)有表達(dá)网站观看jizz，而在受精后釋放極體的受精卵和經(jīng)A23187孤雌激活的卵中則有表達(dá)；在單環(huán)刺螠的早期發(fā)育過(guò)程——二細(xì)胞胚胎国产美女视频免费网站观看、多細(xì)胞胚胎欧美业余性爱、原腸胚、早期擔(dān)輪幼蟲(chóng)——中都有表達(dá)av男人天堂污污污www。