The transgenic tobacco plants could delay TMV infection for about 25 days compared with non-transgenic tobacco plants.Pokeweed antiviral protein II is expressed with high level in summer leaves. The expression of PAPII is regulated by season. The total RNA was extracted from pokeweed (Phytolacca americana L.) leaves in summer using the method of TRIzol and used as template to amplify the PAPII gene by RT-PCR and then the gene was cloned into E.coli expression vector and secreted expression pPIC9K vector. The two vectors with PAPII gene were then transferred into E.coli strain BL21 (DE3)-plysS and Pachia pastor is GS115 strain respectively. The specific protein was produced induced by IPTG and methanol.

由于美洲商陸抗病毒蛋白Ⅱ是一個受季節(jié)調(diào)控表達(dá)的蛋白，本實(shí)驗(yàn)以美洲商陸夏季葉片為材料综合图片亚洲，通過對其總RNA的提取一级毛片aa久久做、反轉(zhuǎn)錄、并用PAPⅡ的特異引物進(jìn)行PCR擴(kuò)增日韩欧美一级A片在线观看网址，對PAPⅡ進(jìn)行了基因克隆一级黄色AV录像、序列分析，結(jié)果擴(kuò)增出來的PAPⅡ基因與已經(jīng)報道的序列同源性是99.9％aaaa片，然后將該基因構(gòu)建到原核A片诱惑视频网址、真核表達(dá)載體上，分別轉(zhuǎn)化了大腸桿菌和畢赤酵母并對它們進(jìn)行了誘導(dǎo)表達(dá)欧美美熟妇激情一区二区三区在线，在兩個表達(dá)系統(tǒng)中均獲得了有活性的PAPⅡ表達(dá)蛋白黄片对白3qA片对白3p，體外生物測定表明表達(dá)的蛋白均具有抑制病毒的活性，PAPⅡ基因在酵母中還沒有表達(dá)的報道，這為獲得具活性PAPⅡ蛋白提供了一種簡便可行的方法毛黄视频。

It is believed that presupposition study within the framework of RT has some theoretic and practical significance.

在對全文內(nèi)容加以總結(jié)的基礎(chǔ)上進(jìn)一步表明語用前提與關(guān)聯(lián)理論相結(jié)合的研究方法的理論和現(xiàn)實(shí)意義日韩精品欧美大片资源在线观看网站。

Methods: the hbv transgenic mice were generated by microinjection of 1.3 copies hbv genome into the pronucleus of fvb/n zygotes. the pcr assay,elisa,rt-pcr and immunohistochemical methods were used to detect the hbv integration,replication and expression in the transgenic mice.

采用受精卵顯微注射法，制備hbv轉(zhuǎn)基因小鼠谁有黄色网站免费，用pcr国产精品爆乳福利在线观看不卡，elisa，熒光定量pcr和免疫組化的方法研究hbv基因在轉(zhuǎn)基因小鼠體內(nèi)的整合www插插插99、復(fù)制和表達(dá)情況国内永久网址。

Using the Hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which Sry transcription can be detected failed to yield any positive results of Xist. Female embryos at the pronucleus stage and 2-cell failed to produce any positive result of Sry and Xist too.

然后利用實(shí)驗(yàn)一確定的PCR條件，以Hprt為陽性對照午夜肥婆，用巢式RT-PCR對小鼠早期胚胎進(jìn)行Xist基因的轉(zhuǎn)錄分析最新a片视频网站，結(jié)果發(fā)現(xiàn)，轉(zhuǎn)錄Sry基因的睪丸組織以及雄性胚胎日韩毛片视频网站中文字幕，從受精卵發(fā)育到囊胚的過程中欧美中文字幕日韩综合四季，基本上不轉(zhuǎn)錄Xist基因；不轉(zhuǎn)錄Sry基因的雌性卵母細(xì)胞和雌性胚胎99riav在线，從出現(xiàn)原核開始欧美又粗又长的A片在线观看，到發(fā)育至2-細(xì)胞期的過程中，Xist基因一直不轉(zhuǎn)錄禁止观看的黄色视频，但是导航在线一区，從4-細(xì)胞期開始，一直到孵化前囊胚階段尤物国产在线精品三区蜜芽，雌性胚胎都轉(zhuǎn)錄Xist基因日韩欧美一级特黄大片视频。

In order to clone the VIP gene in the gastrointestinal tract from beijing duck, one pair of specific primers to VIP gene was designed and synthesized according to the chick sequence (X80906). Encoding VIP cDNA fragments were amplified by RT-PCR from the total RNA in the Proventriculus, the Duodenum and the Jejunum of Beijing duck. Their PCR products were ligated into pGEM-T easy vector, which was transformed into E. coil JM109. Positive bacteria clones were screened and identified by PCR method and digested with the double restriction enzyme EcoRⅠ. The sequence of VIP gene fragment was also determined and analyzed.

為從北京鴨胃腸道中擴(kuò)增血管活性腸肽基因，根據(jù)雞VIP基因（GenBank登錄號X80906）午夜福利欧美性爱，設(shè)計(jì)了一對簡并引物欧爱爱爱网，從北京鴨腺胃、十二指腸和空腸提取總RNA2av天堂网在线观看，通過反轉(zhuǎn)錄－聚合酶鏈反應(yīng)擴(kuò)增网站黄se，將從腺胃、十二指腸和空腸中擴(kuò)增出的產(chǎn)物克隆到pGEM-Teasy載體上亚洲va成无码人在线，導(dǎo)入大腸桿菌JM109国产在线无码制服丝袜无码知名国产，陽性克隆經(jīng)雙酶切鑒定后測序，將測序結(jié)果與雞和鵝（GenBank登錄號為DQ023161）的VIP基因進(jìn)行同源性比較黄色网站久久影院。

The RT-PCR was sensitive and specific for HEV and did not amplify closely related bovine coronavirus and pseudorabies virus of pigs.

檢測與HEV親緣性較高的牛冠狀病毒及豬的偽狂犬病毒均為陰性360毛片视频AA，最低可以檢測到10個TCID50/100μl的病毒，說明該方法的有較好的特異性和敏感性黄色网站在线浏览观看。

The 6 AChE Genes from L.bostrychophila,L.entomophila and L.decolor were demarcated into Insect TypeⅠAChE Gene and Insect TypeⅡAChE Gene.4 Gene cloning of nAChR and mRNA expression level in L.bostrychophilaTwo nicotinic acetylcholine receptor subunit genes,Lb al and Lb a8,were cloned from the psocid L.bostrychophila.The full length cDNAs of Lb a1(GenBank Accession No.: EU871527) and Lb a8GenBank Accession No.

昆蟲的兩類AChE嚴(yán)格的區(qū)分男女猛烈无遮挡羞羞视频，其中Ⅰ型AChE先與脊椎動物AChE聚合后才與Ⅱ型AChE相聚色呦呦av电影，說明兩類AChE基因在物種分化前就已經(jīng)分化。4嗜卷書虱煙堿型乙酰膽堿受體基因克隆及其mRNA表達(dá)水平研究利用RT-PCR和RACE技術(shù)成功克隆獲得了嗜卷書虱2個煙堿型乙酰膽堿受體亞基的全長序列国产淫乱视频，分別命名為Lb a1(GenBank登錄號：EU871527)和Lb a8(GenBank登錄號：EU871526)日本免费网站视频。

It has been difficult to quantitate the amount of specific mRNA without an proper internal standard.

定量RT-PCR技術(shù)的關(guān)鍵之一是必須有適當(dāng)?shù)膬?nèi)參照標(biāo)準(zhǔn)亚日韩黄色网。

RT-PCR was used to quantitate the relative abundance of ndrg2 mRNA expression and analyze the changes.

在小腦手机在线播放黄色网站视频、延髓和海馬等部位，ndrg2 mRNA的表達(dá)水平在16周胎腦中較高国产精品九一精品免费视频。

In addition, the decay rate of early sound energy varies with receiver positions.

同時玖玖资源站在线中文字幕，早期反射聲能在不同位置上的衰減也不均勻，表現(xiàn)在EDT的偏差較RT的大国模高清。