Methods With β-actin gene as a reference, reverse transcriptase polymerase chain reaction was conducted to dectect the expression of PSA mRNA in 35 BC samples, 12 paracarcinoma breast tissues and 10 breast adenofibroma samples.

以β actin作為內(nèi)部參照半定量RT PCR方法檢測 35例乳腺癌組織、12癌旁乳腺組織和 10例乳腺纖維瘤中PSAmRNA的表達(dá)一级A片免费视频国产对白。

It indicated that in rape, six of ABCD genes that are Apetalal, Apetala2, Apetala3, Pistillata, Agamous and Agamous-like11, exhibited a variation in the expression level at the different development stages.

以油菜處于花蕾發(fā)育過程7個(gè)生長期的花蕾和花朵為材料播播午夜，利用優(yōu)化的半定量RT-PCR技術(shù)在轉(zhuǎn)錄水平對ABCD模型的6個(gè)功能基因，即Apetalal黄页网络站大全在线观看污、Apetala2少妇极品欧美日韩视频在线观看、Apetala3、Pistillata97人人操屄网站、Agamous99热在线只有精品99、Agamous-likell，進(jìn)行分析操B网址，探討了油菜花蕾發(fā)育及開花過程中6個(gè)功能基因表達(dá)的差異德国三级片。

We cloned the Rana temporaria chensinensis David skin cDNA using a PCR strategy and detected its activity in rana using a antibacterial protein assay against Micrococcus luteus. The cloning was based on a report of GenBank. The deduced amino acid sequence resulted in 53 amino with 46% identity to amphibia. RT-PCR was used to detect peptide mRNA in Rana temporaria chensinensis David.

我們用提取中國林蛙皮肽總RNA，逆轉(zhuǎn)錄合成cDNA色区一区二区在线观看，PCR擴(kuò)增得到DNA片段91色综合久久熟女，并與GenBank上登陸的其他蛙類的皮肽抗菌肽序列進(jìn)行比較，發(fā)現(xiàn)此序列是一個(gè)新的蛙皮肽抗菌肽序列国产国语一级免费黄色。

RT-PCR amplification of ginseng β-amyrin synthase gene was successfully performed based on the total RNAs extracted from ginseng hairy roots induced by Agrobacterium rhizogenes A4 using a modified guanidine isothiocyanate-method.

以發(fā)根農(nóng)桿菌A4菌株誘導(dǎo)的人參發(fā)根為材料免费试看黄色网站，用改良的異硫氰酸胍法提取總RNA，得到了純度好的完整的總RNA国产大乳一级在线观看。

Expression of a novel antra????poptosis gene, survivin, correlated with tumour cell apoptosis and p53 accumulation in gastric carcinomas［J］.

myc在癌旁正常食管黏膜組織中均未見表達(dá)韩国日本ww色成年免费网站，與多數(shù)文獻(xiàn)報(bào)道一致［1， 7］人人操操操操操操操操操操。survivin為新發(fā)現(xiàn)的凋亡抑制基因在线免费AV，其在食管標(biāo)本中的研究較少，SARELA等［8］及KATO等［9］均用RT中文字幕在线视频永久无码免费视频？欧美性三级视频？？色热av？

Maternal TGFαmRNA was gradually reduced from oocyte to 8-cell stage and maintaining lower TGFαmRNA level was to morula stage, assumably TGFαmRNA transcription was begun from 8-cell stage with the zygote genome activation.

EGF日韩中文有码精品视频在线、TGFα和EGFR mRNA在生殖系統(tǒng)中的表達(dá)通過RT-PCR的方法檢測了EGF、TGFα和EGFR mRNA在生殖系統(tǒng)中的表達(dá)三级视频在线免费观看。

METHODS: 40 rats were randomly divided into four groups:normal group, asthenic spleen group, high-dosage group and low-dosage group with 10 rats in each group, the asthenic spleen rat models were established in group B, C group as well as group D. Rats in group B, group C, group D were fed with normal distilled water, high-dosage of Guizhi decoction and low-dosage of Guizhi decoction respectively, NFAT mRNA, IL-4 mRNA, IFN-γmRNA were determined by PCR.

40只大鼠隨機(jī)分為空白對照組一级毛片免费播放在线视i、脾虛組、桂枝湯大劑量組和桂枝湯小劑量組等4組，每組10只Aa黄色网址；用規(guī)范的脾氣虛證大鼠模型造模方法將B組老熟妇仓乱视频一区二区、C組、D組大鼠造模zz12国产小视频你懂的，B組国外A片无码毛片免费视频、C組、D組分別用蒸餾水国产黄片免费看看、桂枝湯大劑量免费人城网站在线观看、桂枝湯小劑量灌胃，標(biāo)本采集后用RT-PCR檢測NFAT mRNA一本久久，IL-4 mRNA和IFN-γ mRNA表達(dá)水平露出在线。

METHODS: Gastric carcinoma cell line MKN45 at logarithmic growth phase was subcutaneously inoculated in 20 athymic mice.

將對數(shù)生長期的胃癌細(xì)胞株MKN45接種到裸鼠皮下，成瘤后用制備好的分別含GCRG213正毛片全部免费播放无码、反義克隆和RNAi片段的腺相關(guān)病毒分別在腫瘤局部注射欧美A∨在线精品，以空病毒和生理鹽水對照，繼續(xù)喂養(yǎng)2 wk后處死先锋影音最新资源网站，取腫瘤測量重量69毛片免费视频观看，并用半定量RT-PCR檢測腫瘤組織GCRG213mRNA表達(dá)水平。

Methods］ Healthy SD rats were divided randomly into 6 group, including: normal control group(sham-operation), ischemia for 45 min (reperfusion 0h, n=6), reperfusion 2,4,12 and 24 hr group. Renal ischemia reperfusion injury models were founded in other five groups, opened abdominal and placed atraumatic vascular champ in dual kidney pedicle for 45 minutes,followed by reperfusion 2,4,12 and 24 hr.

方法］ 將SD大鼠隨機(jī)分成正常對照組av网站在线管看、單純?nèi)毖M国产丝袜福利在线、再灌注2 h組、4 h組少妇高潮太爽了在线观看、12 h組日韩精品激情视频网站免费观看、24 h組，制備雙側(cè)腎蒂夾閉模型后毛片基地久久伊人91x32.cc，腹主動脈取血測血肌酐亚洲精品自拍私拍、尿素氮，行腎臟組織病理學(xué)檢查自拍国语日韩欧美视频在线播放，免疫組化法檢測TGFβ1 蛋白国内嫖妓无码视频在线观看，RT-PCR法檢測TGFβ1 mRNA。

To establish the base of the control of the Citrus aurantium L. fruit maturity in molecular level and produce high quality Fructus Aurantii, a pair of degenergte primers were designed according to the conserve regions of ACC Oxidase gene of many kinds of plants.

根據(jù)已報(bào)道的各種植物ACC氧化酶氨基酸序列上前后兩個(gè)保守區(qū)設(shè)計(jì)1對簡并引物人人操、人人射、人人爱，以酸橙葉片總RNA為模板优优视频国内A级毛片，通過RT-PCR擴(kuò)增到1個(gè)834bp的基因片段。