Semi-quantitative PCR analysis showed it was expressed in each phase of Jatropha curcas growth.

半定量RT-PCR研究表明該基因在植株的整個生長發(fā)育期都有表達黄色视频中文免费看。

In this study, the complete coding sequence of N-hydroxylating cytochrome P450 (CYP79D2) gene, which catalyzing the committed enzyme in the first step in the biosynthesis of cyanogenic glucoside, was cloned by RT-PCR from cassava.

根據(jù)國際數(shù)據(jù)庫中的木薯氰化物合成限速酶基因序列信息，設計特異引物18岁不能看的网址，通過同源克隆法543ev国产在线，從木薯中克隆出1626bp cDNA基因序列。

At the same time the cell cycles of each group were determined by means of flow cytometer.

采用RT-PCR技術檢測神經干細胞分化過程中GSK-3β国产A片123区、CDK-5和PP2A的mRNA表達一级牲交毛片。

The morphological and cytopathological characters of the viruses were observed by electron microscopy The types of virus were identified with I-ELISA, DAS-ELISA, TAS-ELISA, RT-PCR and IC-PCR. The CP genes of the main viruses identified in this research were amplified by PCR, cloned into pGEM-T and sequenced. The pumpkin germplasm from Heilongjiang and Yunnan provinces was screened for resistance to the main viruses using friction and roots immersed inoculation.

應用電子顯微鏡負染法和超薄切片法觀察病原粒體形態(tài)及細胞病理學特征后λ式吃奶动态后λ式视频；利用酶聯(lián)免疫吸附測定法中的雙抗體夾心ELISA、間接ELISA亚洲爆乳教师av、三抗體夾心ELISA以及反轉錄聚合酶鏈式反應免费无码啪啪视频强奸暴力强奸、免疫捕捉PCR方法鑒定了采集樣品中的病毒種類；對被確定為南瓜主要病毒病原的外殼蛋白基因進行了PCR擴增欧美日韩一区二区三区妖精，克隆到pGEM-T載體并進行測序一级毛片一级毛片一级毛片大全；應用摩擦接種法和浸根接種法對云南、黑龍江省部分南瓜品系多人乱p欧美在线观看、品種進行了抗性篩選一级黄片视频大全在那看。

Consistence to the PT-PCR result, TLR4 protein was strongly expressed at syncytotrophoblast of placenta, cytotrophoblast of chorion and epithelial cell of amnion of pregnancy complicated with syphilis by Immunohistochemistry.

TLR4在妊娠合并梅毒感染的胎盤合體滋養(yǎng)細胞、羊膜上皮細胞和胎膜中的平滑絨毛膜細胞滋養(yǎng)細胞上表達增強亚洲肏屄视频，與RT-PCR結果一致4444亚洲片。

In sham-operation group,animals were decollated and their brains were taken out at 24 hours postoperatively. In all the groups, hippocampal brain injury was detected using histopathological method combined with semi-quantified RT-PCR methods for detecting caspase-3 mRNA. Adult rats aged 90 days were used in modified y maze to examine their learning and memory functions. All these three experiments were independent.

各組海馬區(qū)腦損傷行組織病理學檢測，半胱氨酸天冬氨酸蛋白酶-3 mRNA的表達采用半定量反轉錄-聚合酶反應方法進行檢查免费黄色网99，成年90 d齡的大鼠進行三等分迷宮測試其學習記憶能力国产专区精品主播在线，3個實驗各自獨立。

Methods: Human T cell leukemia cell line Jurkat was chosen as a model. The effect of deguelin on the growth of Jurkat cells was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5 diphenyl-2H-tetrazolium assay. Apoptosis was detected through DNA fragmentation assay, Hoechst 33258 staining assay and Annexin V/PI double-labeled cytometry. The expression levels of nucleophosmin and nucleoporins, including Nup88 and Nup214, were studied by flow cytometry, Western Blot and reverse transcription-polymerase chain reaction.

以人類T淋巴細胞白血病細胞系Jurkat作為研究對象日韩在线你懂的中文字幕，采用MTT法檢測細胞增殖活性亚洲另类三级理论；DNA ladder法、Hoechst33258染色法和Annexin V-FITC/PI雙標法檢測細胞凋亡18黄男人女人色www；流式細胞術在线观看xxxx、Western Blot、RT-PCR檢測魚藤素作用前后日本a网址，Jurkat細胞內核孔蛋白Nup88欧美一区二区日日屄、Nup214與核磷蛋白(nucleophosmin， NPM)表達水平的變化18岁免费欢看黄色网站；激光共聚焦顯微技術觀察上述核孔蛋白與核磷蛋白的亞細胞定位情況98神马午夜。

AIM: To investigate the change of cell cycle checkpoint kinases Chk1/2 in human gastric cancer MGC803 cells induced by diallyl disulfide. METHODS: mRNA expression was assayed by means of RT-PCR in MGC803 cells treated with DADS.

目的：觀察二烯丙基二硫化物對人胃癌MGC803細胞的細胞周期檢查點激酶1(Chk1)和細胞周期檢查點激酶2(Chk2)的影響。

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In additional, eIF5a2 is located on chromosome 2B using Langdon D-genomic disomic substitution lines and the expression of eIF5A was also investigated by Semi-quantitative PCR.

進一步研究表明eIF5a2位于2B染色體上，并用半定量RT-PCR 研究了小麥eIF5A基因的表達情況九九C片一级黄片。