The answer was that the former could be far sensitive than the latter, but the former needed complex instruments. The latter could help us to directly judge the order of severity by colors.On the other hand, we took advantage of polymerase chain reactionto amplify the MCP genome of the virus and compare it with other nine published MCP genomes of viruses including CzIV, TIV, CIV, LCDV-1, LCDV-K1, BIV, FV3, GIV, RSIV. The sequencing data showed that the MCP of Cobia iridoviruswas more homologous to the MCP of LCDV-1（89﹪similarity）than that from FV3（47﹪similarity）.

另一方面亚洲最大偷拍视频，為了了解引起海鱺病變之病毒究竟屬於何種病毒黄色视频在线aaaaaa，我們透過(guò)聚合酶鏈鎖反應(yīng)增幅其主要鞘蛋白基因并加以定序一级黄色视频免费观看，與其他九種已發(fā)表的虹彩病毒之 MCP 核酸及胺基酸序列亚洲男女日逼视频网站，包括：CzIV、TIV99精品综合加勒比在线观、CIV亚洲欧美色图13p、LCDV-1、LCDV-K1欧洲免费AV在线网站观看、BIV国产综合视频网、FV3、GIV国内妇人成熟A片免费看、RSIV 之 MCP 進(jìn)行分析比對(duì)亚洲第一区在线观看，顯示 Cobia iridovirus親緣性和感染大多數(shù)硬骨魚(yú)類的 Lymphocystivirus 屬的 LCDV-1 最為接近（89﹪相似度），和感染兩棲類的 Ranavirus 屬的 FV3 親緣性較低（47﹪相似度）久久久久精品久久久久免费。

We identified the likely disease-causing mutation by applying genomic methodologies (multiplex target amplification and massively parallel sequencing) to historical specimens from the Romanov branch of the royal family.

為了弄清該疾病的確切性質(zhì)十八女免费毛片，研究人員從最近發(fā)現(xiàn)的俄國(guó)Romanov家族的遺骸中提取了已經(jīng)降解的DNA樣本，其中包括維多利亞的患有血友病的曾孫Alexei王儲(chǔ)的DNA樣本人人干97。

We identified the likely disease-causing mutation by applying genomic methodologies (multiplex target amplification and massively parallel sequencing) to historical specimens from the Romanov branch of the royal family.

我們從皇室家族Romanov一支取得樣本一级毛片特黄久久免费看按磨站，通過(guò)遺傳學(xué)方法（多元靶點(diǎn)擴(kuò)增和大量平行測(cè)序）確定了可能的致病性突變。

In this study, 2 individuals carrying RHD positive, D antigen negative allele (with exon 1 and 10 of RHD) and their mRNA were investigated by using reverse transcriptase PCR and sequencing through one pair of specific primers for 5′- and 3′-non-coding region of RHD, taking a Rh-positive sample as control.

本文分別針對(duì)RHD基因mRNA的5′-和3′-非編碼區(qū)設(shè)計(jì)一對(duì)特異性引物免费一级性交黄片无码，通過(guò)逆轉(zhuǎn)錄PCR和cDNA測(cè)序永久免费男男在线观看全网站，分析2例RHD基因陽(yáng)性（擁有第1和第10外顯子）、D抗原表型陰性個(gè)體的全長(zhǎng)mRNA/cDNA序列日韩精品图片观看网址大全下载，同時(shí)以1例正常Rh陽(yáng)性個(gè)體作對(duì)照久久久久精品超国产。

The sensibility and specificity of the method were evaluated with the amplification and by sequencing randomized Rh-negative and Rh- positive controls.

序列特異性引物方法擴(kuò)增RHD 基因特異的外顯子及其側(cè)翼序列，PCR產(chǎn)物直接序列分析測(cè)定核苷酸的變異人人操人人插人人看人人爱。

The apxⅢA gene of Actinobacillus pleuropneumonie was amplified by PCR. The amplified DNA fragment 3 466bp was cloned into pMD18-T. After R.E. analysis and sequencing, the apxⅢA gene in pMD18-T was ligated into pBluescripⅡSK, the recombinant expression plasmid pET-28b/apxⅢA was constructed and analysed with R.E., the protein of apxⅢA gene expressed in E.coli BL21 was detected by Western blotting.

因胸膜肺炎放線桿菌的致病性主要是由毒素決定的好妞在线三级片，故參照豬胸膜肺炎放線桿血清2型菌株的序列(GenBankL1 2 1 4 5)設(shè)計(jì)了一對(duì)特異性引物，用PCR的方法擴(kuò)增apxⅢA基因并得到了長(zhǎng) 3 466bp的片段人人操人人超，然后將其克隆到pMD 1 8T中99久久久国产一区二区三区，經(jīng)酶切鑒定和序列分析表明克隆是成功的；再將apxⅢA插入到原核表達(dá)載體pET 2 8b后欧美www视频在线观看，轉(zhuǎn)化BL2 1 (DE3)欧美大屌插逼，在IPTG誘導(dǎo)下獲得高效表達(dá)，經(jīng)Westernblotting檢測(cè)證實(shí)表達(dá)產(chǎn)物有活性日韩精品免费观看大全网站。