The plasmid of pMD-T-GO and pPIC3.5K were extracted from JM109 strain and then glycolate oxidase gene fragment was cloned into the expression vector (pPIC3.5K). The recombinant plasmids were transformed into E.coli TOP 10Fˊ. The recombinant clones were identified by PCR, digested with SnaB I /Not I and then sequenced.

本文首先從大腸桿菌JM109中提取質(zhì)粒pMD-T-GO和pPIC3.5K黄页网站在线全部免费观看，將菠菜乙醇酸氧化酶基因c DNA片段克隆至表達載體pPIC3.5K九九自拍偷拍，轉(zhuǎn)化進E.coli TOP 10Fˊwww.日本免费看，利用設(shè)計的引物進行PCR擴增aaaaaa爱爱、SnaB I和Not I酶切鑒定，最后進行序列分析好硬好爽好大高潮不断视频。

Or I will snab her necks.- No way, we don't give her a shit.

否則我掐死她-沒門兒我們才不管她呢

PCR productsdigested by SnaB Ⅰ and Not Ⅰ were inserted into the correspondingrestriction site in the expression plasmid pPIC9K.

用限制性內(nèi)切酶 SnaBⅠ和 NotⅠ酶切 NGF cDNA 的 PCR 產(chǎn)物4444在线视频看片，將其插入到載體 pPIC9K 中相應(yīng)的限制性內(nèi)切酶位點，構(gòu)建表達載體pPIC9K-NGF.3亚洲免费操。

Methods Digest recombinant plasmid pU57-SOD with SnaB Ⅰ and Not Ⅰ and insert the obtained SOD gene into expression vector pPIC9k.

方法根據(jù)酵母密碼子偏好性www.pylng.com，合成人Cu，Zn-SOD基因18r免费网址，克隆至真核表達載體pPIC9k中人人看人人爽，轉(zhuǎn)化畢赤酵母GS115。

Several fragments containing the replication origin were cloned into replicon probe vector pUC-MEV constructed in this study. After screening a 4. 8kb PvuⅡ-SnaBⅠ fragment were determined to be the minimum replication region of the plasmid.

將包含復制起始原點在內(nèi)的數(shù)個片段分別插入構(gòu)建的嗜鹽古生菌的復制子探針載體pUC-MEV中欧美国产88，通過篩選得到了pHH205最小復制功能片段。

A SnaBⅠand NotⅠrestriction sites were introduced into NGF generespectively by PCR .After being amplified by PCR,this NGF genecontained SnaB Ⅰ and Not Ⅰ restriction sites at its 5'and 3'endsrespectively.

設(shè)計合成一對特異引物自拍视频日韩在线视频观看免费网站，分別帶有 SnaBⅠ和 NotⅠ的限制性內(nèi)切酶的識別位點99色视频在线播放，以 pPIC9K-NGF重組質(zhì)粒為模板，用 PCR 方法擴增 NGF cDNA 片段国产成年女AV产免费传媒。

All of the 58 restriction endonucleases were used to investigate the mtDNA diversity. Four restriction endonucleases, DraⅠ40XXOO.com、SnaBⅠ、SspⅠ and Vsp Ⅰ, were polymorphic. Based on presence or absence of the the 215〓 SspⅠ site, all of the 13 mtDNA sequences were divided into two groups.

利用DNAClub軟件對AT富集區(qū)和tRNA〓基因的DNA序列進行了模擬酶切亚洲日韩欧美福利区一久，所有的58種內(nèi)切酶中有10種內(nèi)切酶在13個材料中有酶切位點h色视频观看在线观网站，僅4種酶DraⅠ、SnaBⅠ久久这里的视频都是精品、SspⅠ和VspⅠ表現(xiàn)出多態(tài)性操操操人人人，且主要位點差異在于SspⅠ酶切位點的有無（序列的第215位的C/T）。12個品種中有4個品種（豫早1號黄色网岛国、豫早2號特级婬片老女人高清视频、魯紅、33）沒有此酶切位點男女操逼视频网址国产，另外8個品種有此酶切位點射天堂中文字幕色，野柞蠶也沒有此酶切位點。

To prepare recombinant fox growth hormone, we amplified its cDNA from silver fox pituitary tissue by RT-PCR and cloned into yeast shuttle vector pPIC9K down stream of α-factor signal peptide sequence by SnaB I and Not I restriction sites. The recombinant secretion vector pPIC9K/fGH, linearized by Sal I, was transformed into histidine-deficient Pichia pastoris strain GS115 by electroporation.

為制備重組狐貍生長激素在线推荐一个可以看黄色av的网站，采用RT-PCR方法国产小黄片入口，從銀狐垂體中擴增fGH cDNA基因，利用SnaB I和Not I位點將fGH基因插入到酵母分泌型表達載體pPIC9K中α-因子信號肽的下游久久91精品国产，構(gòu)建成fGH基因的酵母分泌型表達載體pPIC9K/fGH欧美黄色一级片视频，載體經(jīng)Sal I酶切線性化后91九色在线播放国产日韩，通過電轉(zhuǎn)移將線性化的pPIC9K/fGH轉(zhuǎn)化到組氨酸缺陷型酵母宿主菌GS115中。

Methods Amplify IL-6 (23)-PE40 gene from the previously constructed recombinant plasmid pKK-IL-6(23)-PE40 by PCR, and insert to the SnaB Ⅰand NotⅠ sites of vector pPIC9. Transform the constructed recombinant plasmid pPIC0-SN to P. pastoris GS115 by electroporation, screen Mut-recombinants for expression under induction of methanol.

采用PCR技術(shù)求AV在线网站，將目的基因IL-6(23)-PE40插入到pPIC9載體中SnaBⅠ與NotⅠ位點在线观看A毛片，電轉(zhuǎn)化至巴斯德畢赤酵母GS115中，篩選Mut-型重組酵母天天天爽天天天干天天天舔；甲醇誘導表達www,东京热,com，體外細胞試驗檢測其細胞毒性。