Adding Ficoll to centrifugalize, BMSCs were isolated, cultured and proliferated. The intestinal segment about 15 mm×5 mm was obtained sterilely from fetal duodenal papilla to colon, then muscular tunic and adventitia were peeled.

收集四五個(gè)月流產(chǎn)胎兒標(biāo)本6例的四肢骨髓，F(xiàn)icoll離心538午夜视频，分離瑟瑟的网址、培養(yǎng)、擴(kuò)增獲取骨髓間充質(zhì)干細(xì)胞影音先锋啪啪啪。

METHODS: Full-thickness skin was sterilely obtained from rabbit ears. 2 cm×2 cm free skin graft was placed in 0.25% pancreatin-EDTA to remove epidermis, in Qulatong X-100 solution to completely take off hypodermal cells, repeatedly rinsed in phosphate buffer saline. Thus, acellular dermal matrix was collected, and kept at 4 ℃ for usage.

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METHODS: Neonatal mouse brain tissues were sterilely obtained to prepare monoplast suspension.

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METHODS: Fetal liver was obtained sterilely. Monoplast suspension was collected by collagenase digestion and mechanical separation, and then centrifuged using 1.070 g/mL Percoll separating medium and 1.077 g/mL Ficoll separating medium. Cells achieved from the interface of separatory liquids were cultured in DMEM/F12 medium, supplemented with 0.1 volume fraction of fetal bovine serum, 20 μg/L hepatocyte growth factor and 40 μg/L epidermal growth factor, for 9 days.

無菌狀態(tài)下取出胎兒肝臟，以膠原酶消化法和機(jī)械分離法獲得胎兒肝臟來源單細(xì)胞懸液黄色a级，分別采用1.070 g/mL Percoll分離液和1.077 g/mL Ficoll分離液進(jìn)行密度梯度離心亚洲欧美性交图片，吸取界層細(xì)胞，添加含體積分?jǐn)?shù)為0.1 FBS日韩wuma、20 μg/L肝細(xì)胞生長因子欧美在线公开视频、40 μg/L表皮生長因子的DMEM/F12新鮮培養(yǎng)基誘導(dǎo)9 d。

METHODS: Rat cerebral cortex was obtained sterilely, made into monoplast suspension by the mechanical separation, and then incubated in the serum-free MEM/F12 medium, supplemented with B27 neurotrophic additive for 1 week. Subsequently, cells were passaged.

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METHODS: Olfactory bulb was sterilely collected from rats, digested, and centrifuged. After removal of the supernatant, the olfactory bulb was resuspended with DMEM/F-12 medium containing 0.2 volume fraction of fetal bovine serum. The diluted monoplast suspension was equally assigned into 3 samples, and separately used for simple modified differential adherence, simple serum-free conditioned medium, and modified differential adherence + serum-free conditioned medium.

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ML of bone marrow was sterilely aspirated from the posterosuperior iliac spine of 36 healthy mongrel dogs. BMSCs were isolated and purified by Percoll density gradient centrifugation and cultured in vitro by adherence method.

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All patients received a massive bone allograft that had been sterilely procured without secondary irradiation.

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METHODS: Bone marrow was sterilely isolated. BMSCs were isolated and purified by the density gradient centrifugation.

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All patients receied a massie bone allograft that had been sterilely procured without secondary irradiation.

所有的病人都接受了經(jīng)消毒而未進(jìn)行二次輻射的大塊同種異體骨色色色視頻。