METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

無菌抽取的骨髓經(jīng)肝素化后，采用密度梯度離心法及貼壁篩選法分離獲得人骨髓間充質(zhì)干細胞超碰97中文无码亚洲，傳至第5代按1× 108 L-1接種于6孔板內(nèi)操网址，設(shè)立2組，依達拉奉組細胞達50%融合時用含堿性成纖維生長因子欧美日韩操逼、胎牛血清的L-DMEM預(yù)誘導(dǎo)24 h，PBS洗滌后再用20 mg/L依達拉奉無血清L-DMEM誘導(dǎo)24 hjizz国产黄色视频；空白對照組始終用含體積分數(shù)為10%胎牛血清的L-DMEM培養(yǎng)国产一乱一伦一情，不加任何預(yù)誘導(dǎo)劑和誘導(dǎo)劑。

METHODS: Rat double lower limbs were isolated sterilely. Bone marrow was obtained by rinsing using low-glucose DMEM. BMSCs were isolated and purified by the whole bone marrow adherence method and density gradient centrifugation.

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METHODS: Bilateral sciatic nerve was sterilely dissociated from rats. Schwann cells were prepared and purified by low-concentration enzyme fast digestion and differential adherence.

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METHODS: Bilateral testis of Kunming male mice aged 5-7 days were sterilely collected. Spermatogonial stem cells were isolated and purified using adherence and noncontinuity Percoll density gradient centrifugation.

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METHODS: Umbilical cord blood samples were sterilely isolated using Percoll density gradient centrifugation to harvest intermediate layer cells. DMEM medium containing fetal bovine serum, penicillin, streptomycin and L-glutamine was added. Following several adherences and purification, the floating cells were discarded. Thus, many adherent cells with a confluence were collected. When cells were 60%-80% confluent, cells were digested by trypsin for subculture.

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METHODS: Human AMSCs were isolated in vitro sterilely. At the third passage, 0.5 mL single cell suspension at 1×109/L was obtained and transplanted by tail venous pathway in transplantation group mice, Mice in the control group were injected with an equal volume of saline. APP-gene mice in the normal group were left intact. 5'-bromo-2-deoxyuridine labeled third-generation AMSCs expression was detected in mice brain tissue by immunohistochemical method.

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