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Marine birnavirus, a member of the genus Aquabirnavirus, family Birnaviridae, is an unenveloped icosahedral virus with two genomes of double-stranded RNA.

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By induction of IPTG, the E.coli with pET28a-hnRNPA2/B1 expressed the fusion protein ,which stranded at the 41KD by SDS-PAGE analysisthe comparing to E.coli uninduced and the control .

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Structural comparison shows that this loop selectively competes with the unmethylated strand of the DNA duplex for binding to FTO, suggesting that it has an important role in FTO selection against double-stranded nucleic acids.

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The genome of avian adeno-associated virus was cloned for construction of gene transfer vectors. AAAV was propagated in special pathogen-free chicken embryo using the chicken embryo lethal orphan virus as the helper virus. The double-stranded genomic DNA was extracted from precipitated AAAV viron and cloned into pCR 2.1 vector.

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The point mutations of the 12th coden of ras gene and p53 E5 and E7 gene were studied in 40 cases w ith laryngeal squamous cell carcinomas by PCR-SSCP(polymerase chain reaction-s ingle stranded conformational polymorphism).

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Had been airlifted to safety over the weekend, leaving about 1,000 still stranded.

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