Methods ROLTs were performed according to Kamada's two cuff technique with some modifications in donor liver acquirement, perfusion, cuff made, recipient anesthesia, anastomosis of suprahepatic inferior vena cava and shorten anhepa...

材料和方法：應(yīng)用Kamada&二袖套法&行大鼠肝移植，在既往國(guó)內(nèi)外文獻(xiàn)報(bào)道的基礎(chǔ)上對(duì)供肝切取视频一区无码天堂、灌注www,日本高清视频、袖套管制作、受體麻醉色一区二区三区、肝上下腔靜脈吻合和縮短無肝期等方面進(jìn)行改進(jìn)黄色小视频网站网址在线免费观看，熟練實(shí)驗(yàn)操作。

Methods: The model was established with two cuff technique; The donor was perfused through the abdominal aorta with cold perfusate; The anastomosis of the suprahepatic vena cava was sutured end to end with 8~0 nylon line, and the continuity of portal vein and infrahepatic vena cava were established by means of cuff method respectively. The bile duct anastomosis was performed with internal stent.

采用二袖套法行大鼠原位肝移植国产A片一级黄色，供體經(jīng)腹主動(dòng)脈進(jìn)行肝臟冷灌注青青操在线视频，肝上下腔靜脈采用手縫合法進(jìn)行吻合；門靜脈和肝下下腔靜脈用袖套法吻合扒开双腿疯狂进出爽爽爽视频；膽總管采用單管內(nèi)支架膽管端端吻合久久久国产带黄Av。

The donor liver was cold perfused through abdominal aorta and portal vein respectively, the liver of recipient was step-excised, the suprahepatic vena cavewas sutured end -to -end with 8-0 nylon line, and the continuity of infrahepatic VC and portal veinwere established by means of cuff method respectively.

作者在參考Kamada等[1，2]用&二袖套法&吻合血管的基礎(chǔ)上免费国产乱码黄片，并對(duì)供肝的灌注欧美又大又粗、受體肝臟切除及移植肝臟的血流重建等進(jìn)行改進(jìn)，以提供一個(gè)穩(wěn)定可靠的動(dòng)物模型的建立方法99级A片。1材料與方法1.1實(shí)驗(yàn)動(dòng)物网站自拍偷拍、藥品及術(shù)前準(zhǔn)備采用健康、成年在线观看国产mm免费视频、雄性欧美爱性真人在线视、清潔級(jí)體重約200￣300g SD大鼠和二級(jí)Wistar大鼠，飼養(yǎng)條件符合SPF標(biāo)準(zhǔn)由南方醫(yī)科大學(xué)南方醫(yī)院實(shí)