Plutella xylostella larvae of a strain highly resistant to spinosad were used as hosts to rear the parasitoid Cotesia plutellae. When the parasitoids had developed to the stage of early instar larva, the host larvae were treated with 50 mg/L spinosad on the terga by topical application and then fed with cabbage leaves treated with 50 mg/L spinosad to allow the host larvae and the parasitoid larvae to develop. Three days after treatment, the parasitoid larvae inside the host were collected by dissecting the host larvae. The body fluid of P. xylostella and the bodies of parasitoid larvae were then analyzed with HPLC. Both of the active components, spinosyn A and spinosyn D, of spinosad were detected in the body fluid of host larvae and in the bodies of parasitoid larvae, and the residue concentrations were 2.79 mg/L and 0.94 mg/L in the host and the parasitoid respectively.

以對多殺菌素高抗的小菜蛾P(guān)lutella xylostella幼蟲作為菜蛾絨繭蜂Cotesia plutellae的寄主，待絨繭蜂發(fā)育到1齡幼蟲時亚洲福利精品一区，將濃度為50 mg/L的多殺菌素點滴到寄主幼蟲背板上中日韩av电影，隨后讓寄主幼蟲取食經(jīng)50 mg/L多殺菌素處理過的甘藍葉片，寄主幼蟲和其體內(nèi)的蜂幼蟲再發(fā)育3天后亚洲xx视频，將寄主幼蟲解剖取出蜂幼蟲国产精品91在线播放，用高效液相色譜法對經(jīng)多殺菌素處理的小菜蛾幼蟲體液以及絨繭蜂幼蟲勻漿液進行檢測，結(jié)果多殺菌素的2個活性成分spinosyn A和spinosyn D均被檢測到国产美女久久精品香蕉69，兩者的多殺菌素殘留濃度分別是2.79 mg/L和0.94 mg/L久久综合无码一区丝袜。