The recombinant enzyme hydrolyzed p-nitrophenyl esters but not tributyrin and tricaprylin, and exhibited the highest specific activity (1.1 μmol min-1 mg-1) to p-nitrophenyl caprylate.

SsoPEst不水解三酰甘油酯www.认真日本，可以水解對(duì)硝基苯酚酯黄色视频特级免费欧美A片，對(duì)對(duì)硝基苯酚辛酯水解活力最高美女免费视频一区，為1.1 μmol min-1 mg-191国在线视屏，催化對(duì)硝基苯酚酯水解的最適pH為5.5日韩在线看片网址，最適溫度為80 °C精品在线电影，酶溶液置于80 °C 熱處理1小時(shí)仍有60 %活力。

Objective To investigate the differentiation of NB4 and MR2 cells induced by tributyrin in combination with different doses of all-trans retinoic acid and to see if TB can induce apoptosis of NB4 and MR2 cells.

目的 研究三丁酸甘油酯與全反式維甲酸不同濃度組合對(duì)NB4及MR2細(xì)胞的誘導(dǎo)分化作用欧美啪啪小视频，并觀察TB對(duì)NB4一级性做久久久久久、MR2細(xì)胞是否具有促凋亡作用。

Lipase production on agar medium was detected by the tributyrin diffusion plate assay.

單甘酯合成試驗(yàn)表明菌株D2所產(chǎn)的脂肪酶具備合成單甘酯的能力欧美黄片亚州黄片。

The most common methods used for screening of lipase-producing strains are observation of transparent circle on tributyrin agar plate and color-changing circle on olive oil agar plate.

在脂肪酶產(chǎn)生菌的篩選中日韩美欧自拍视频播放在线观看免费，最常用的方法是三丁酸甘油酯瓊脂平板透明圈法和橄欖油瓊脂平板變色圈法。

However, the recombinant yeast could not grow on the traditional tributyrin plate because this kind of medium is lack of essential nutrients for yeast growth.

而傳統(tǒng)的三丁酸甘油酯平板缺乏有效營(yíng)養(yǎng)成分女人高潮A级毛片，重組酵母無(wú)法生長(zhǎng)国产操女人；橄欖油平板靈敏度較低，低脂肪酶活力的重組酵母不能產(chǎn)生變色圈办公室强奷在线播放AV，這給產(chǎn)脂肪酶基因工程菌的鑒定帶來(lái)了困難中文字幕日韩国产精品强奸乱伦。

In conclusion, the mechanism of differentiation and apoptosis in NB4, K562 cells induced by tributyrin may associate with its up-regulation of acetylated histone and p21(superscript WAF1) mRNA level.

TB誘導(dǎo)NB4、K562細(xì)胞發(fā)生分化和凋亡的機(jī)制與TB引起細(xì)胞組蛋白高乙魽吖在线视频；屠^而上調(diào)p21(上標(biāo) WAF1)表達(dá)有關(guān)亚洲美女模特一级A片。

We use plate to check the lipase activity ,the lipase activity is very low, Compared to the wild type , the mutant could not form the clear halo on the olive oil plate but tributyrin plate, it showed that the 227 th Serine was essential for the lipase activity.

與野生型相比，突變體脂肪酶的酶活很低一级男女啪拾起来，不能在橄欖油平板上形成透明圈一级作爱片免费，但可以在以三丁酸甘油酯為底物的平板上形成透明圈，表明第227位絲氨酸可能對(duì)脂肪酶酶酶活具有重要影響。

We detected the distribution of lipases in 35 Aeromonas isolates by PCR andphenotypic test using tributyrin as substrate. The positive rates were 100% and 83%,respectively. The lipase activity or associated genes could be detected in the two avirulentstrains, A. hydrophila W1 and A. hydrophila 4332. It suggested that lipase might not be animportant factor in pathogenic mechanism of A.

對(duì)本實(shí)驗(yàn)室保存的35株氣單胞菌分離株進(jìn)行了脂酶的表型檢測(cè)和PCR檢測(cè)欧美一级A级黄片，結(jié)果分別為83％和100％222aaa男人的天堂免费网址，特別是嗜水氣單胞菌無(wú)毒株W1和Ah4332也能檢測(cè)到脂酶活性或基因，表明脂酶在該菌的致病機(jī)制中不是決定性因子欧美在线精品一区二区三区。

One positive clone was obtained from the libraries with the tributyrin flat and ELISA sieve method. Analyzed the sequencing result, the positive clone include the Open Read Frame of low-temperature alkaline lipase gene.

序列測(cè)定分析表明特级无码毛免费视频，此重組質(zhì)粒包含有長(zhǎng)度為此95lbp的低溫脂肪酶基因的完整的開放讀碼框架和上游調(diào)控序列。

A strain NK13 was screened for a certain extent asymmetric hydrolyze the rac-ketoprofen Chloroethyl ester and identified as Bacillus megaterium. For the preparation of gene libraries, a positive clones was obtained from the tributyrin flat. The sequence of this esterase gene had been analysised, and contained the whole ORF of an esterase gene with the length of 933bp.

以本實(shí)驗(yàn)室篩選出的一株具有不對(duì)稱拆分消旋酮基布洛芬氯乙酯的菌株NK13為材料16wv.com免费，經(jīng)初步鑒定為巨大芽孢桿菌一级A片操逼，通過(guò)構(gòu)建其基因文庫(kù)，從中篩選得到一陽(yáng)性克隆重組子pUC-NK欧美xxxx18。