The activity of lipasc from S78- pVT102U/a -lip was up to 20U/ml after 72h fermentation; The product of S78-pVT102U/a-L-lip07 was little, only formed the halo on the tributyrin plate.

其中www.好了av.com，重組酵母S78-pVT102U／α-lip搖瓶發(fā)酵72h，酶活可達(dá)20U／ml操操操草草草；S78-pVT102U／α-L-lip07表達(dá)的脂肪酶很少在线网址你懂，表達(dá)產(chǎn)物僅在三丁酸甘油酯平板上形成透明圈。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

該菌株經(jīng)IPTG誘導(dǎo)可表達(dá)分子量約80 kDa的融合蛋白综合人成精品玖玖玖在线，SDS-PAGE分析表明融合蛋白位于菌體超聲裂解后的上清中国产毛片爽爽影院无码，用蛋白純化試劑盒HisTrap~ HP純化后得到單一的目的蛋白，約80 kDa亚洲国产欧美日韩另类，且純化的融合蛋白具有脂酶活性最新黄色欧洲网站，可以分解三丁酸甘油酯。

In this study, isolate 23 microbes with lipase activity from Wu-Jie scrap heap at Ilan and Taichung Environmental Protection Section. When the microbes incubator in 50oC, there are isolates B61 and M63 that lipase activity are better than others. Brevibacillus borstelensis SH168 isolated from NTU resterant food waste compost is used as control check. Isolates B61, M63 and Brevibacillus borstelensis SH168 determine the lipase activity by the LB with tributyrin. The lipase activity of isolates B61 and M63 are 2~3 fold higher than Brevibacillus borstelensis SH168. So isolates B61 and M63 are more useful in producing lipase. Isolate B61 can grow at pH 5~9, and at temperature 20oC ~ 60oC; isolate M63 can grow at pH 6~10, and at temperature 30oC ~ 50oC. Isolates B61 and M63 were identified by 16S rRNA. Isolate B61 is Bacillus circulans, and isolate M63 is Bacillus species.

本研究天天色天天日天天日，於宜蘭五結(jié)垃圾場(chǎng)和臺(tái)中市環(huán)保局廚余堆肥樣品中共分離出 23 株具脂肪酶活性菌株黄色一级视频网址， 23 株菌株中經(jīng)過(guò)三油酸甘油酯的洋菜培養(yǎng)基於 50℃培養(yǎng) 5 天后，發(fā)現(xiàn)有 2 株菌的脂肪酶活性較強(qiáng)色色资源站免费视频，分別為分離株 B61 和 M63片免费AA片线观看；以本實(shí)驗(yàn)室先前自臺(tái)大女九餐廳蔬果廚余堆肥中所篩選出的耐高溫脂解菌 Brevibacillus borstelensis SH168作為本實(shí)驗(yàn)對(duì)照菌株，將分離株 B61久久久精品国产免费观看一区二区、M63 及 Brevibacillus borstelensis SH168 3 株菌株於含有三酪酸甘油酯液態(tài)培養(yǎng)基培養(yǎng)并測(cè)定其脂肪酶活性91在线国产福利亚洲，發(fā)現(xiàn)分離菌株之活性皆比 Brevibacillus borstelensis SH168 高出 2~3 倍，認(rèn)為分離株 B61人人摸人人做人人看、M63 適於產(chǎn)生脂肪酶的菌株人人操人人插人人玩，分離株 B61 在 pH 5~9 皆可生長(zhǎng)，生長(zhǎng)溫度介於 20 oC ~ 60oC免费一级A片国产在线观看牲奴；分離株 M63 在 pH 6~10 可生長(zhǎng)日韩免费视频网站，生長(zhǎng)溫度介於 30 oC ~ 50oC； 2 株分離菌株以 16S rRNA定序結(jié)果分離株 B61 為 Bacillus circulans在线首页日韩精品视频网站，分離株 M63 為 Bacillus 屬日韩精品99密。