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Thecompare of genetic map between Lowes and ours showed 26 homology marker situ,which occupied 21.1% of the marker situ in the experiment. 81 QTLs were detected for 11 agronomic traits. 4 QTLs were detected for plantheight, which explained 10.3%~28.9% of trait variance; 2 QTLs were detected forNo. of effective 1-st branches, which explained 22.1%~47% of trait variance; 16QTLs were detected for effective branches height, which explained 12.2%~51.8% oftrait variance; 15 QTLs were detected for length of main inflorenscence, whichexplained 7.4%~26.6% of trait variance; 5 QTLs were detected for effective siliquesof main inflorenscence, which explained 11.2%~25% of trait variance; 1 QTLs weredetected for density of main infiorenscence, which explained 17.3% of trait variance;12 QTLs were detected for length of silique, which explained 24%~36.7% of traitvariance; 2 QTLs were detected for seed per sillique, which explained 9.6% and16.9% of trait variance; 2 QTLs were detected for 1000 seed weight, which explained26%~13.7% of trait variance; 11 QTLs were detected for Total effective siliques perplant, which explained 14.8%~47.2% of trait variance; 11 QTLs were detected forplant height, which explained 14.3%~32.8% of trait variance.
其中,株高檢測到4個QTLs在线一级油黄片,解釋性狀表型變異的10.3%~28.9%五月婷婷丁香综合伊人一区二区三区四区;一次有效分枝數(shù)檢測到2個QTLsjjzzjizz中国,解釋性狀表型變異的22.1%和47%26uuu欧美杂交;有效分枝部位檢測到16個QTLs国产92AV视频,解釋性狀表型變異的12.2%~51.8%;主花序長度檢測到15個QTLsav超碰人人操,解釋性狀表型變異的7.4%~26.6%;主花序有效角數(shù)檢測到5個QTLs亚洲欧美一区日韩一区a国产传媒,解釋性狀表型變異的11.2%~25%;主花序角密度檢測到1個QTLs插女人视频,解釋性狀表型變異的17.3%色色色wang;角果長度檢測到12個QTLs,解釋性狀表型變異的24%~36.7%;每角粒數(shù)檢測到2個QTLs亚洲,日韩,性爱,解釋性狀表型變異的9.6%和16.9%影音先锋女人av鲁资源网;千粒重檢測到2個QTLs,解釋性狀表型變異的26%和13.7%欧美日韩精品一二;單株有效角果總數(shù)檢測到11個QTLs看男女搞,解釋性狀表型變異的14.8%~47.2%;單株產(chǎn)量檢測到11個QTLs亚洲美女日逼A片,解釋性狀表型變異的14.3%~32.8%高潮黄网站色在线观看免费。
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There were high density images shaped as rope and slip in lungs of model control group rats and the pleura near the images were a little thickening. In MTX treated group, there were high density images shaped as slice along pulmonary veins. The veins of the blood vessels were obscure and the edges were unclear. In TPT treated group,there were multiple high density images shaped as tubercle and slice and the boundary were not clear. In XFC treated group, the lungs' outlines were clear and the veins were welldistributed. High density images shaped as spot were found in part lungs.
結(jié)果:HRCT表現(xiàn)為模型對照組大鼠肺輪廓欠清晰,肺部血管影擴張2020亚洲一级兔费A片,邊緣模糊且不規(guī)則欧美在线播放中文字幕,兩肺有索條狀密度增高影,鄰近胸膜輕度肥厚免费无码高清黄色网址;甲氨喋呤組大鼠肺部可見沿著肺紋理分布的斑片狀密度增高影久久国产香蕉毛片一级,肺血管紋理模糊,邊緣不清楚欧美综合首页;雷公藤多苷片組大鼠兩肺可見多發(fā)性結(jié)節(jié)狀及斑片狀密度增高影日韩性交免费A级黄片,境界欠清晰;新風膠囊組大鼠肺輪廓清晰欧美日韩无线码一区二区,肺紋理分布均勻色夜在线,部分肺組織顯示斑點狀密度增高影。
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Results: Amang 128 patients, 56 cases were diagnosed as pulmonary carcinoma (43.75%), of which 30 cases were squamous carcinoma (53.57%), 22 cases were adenocarcinoma (39.29%), 4 cases were small cell undifferentiated carcinoma (7.14%); 37 cases were bronchial pneumonia (28.91%), in which 30 cases were given brush sampling, bacterial culture of BALF, the detectable rate of pathogenic bacteria were 50%(15 cases) and 40%(12eases) respectively; 12 eases were pulmonary tuberculosis (9.38%), of which 3 eases (25%) had concurrent endobronchial tuberculosis, smear of brush biopsy and BALF found Mycobacterium tuberculosis were 11 eases (91.67%) and 5 eases (41.67%) respectively, 2 eases were diagnosed as caseous tubereulous granuloma (16.67%); 2 eases were idiopathic pulmonary fibrosis (1.56%), lease was lymphoma of long.
結(jié)果:128例患者確診為肺癌56例(43.75%)欧美性视频在线,其中鱗癌30例(53.57%)www.韩日欧黄色片网站.com,腺癌22例(39.29%),小細胞癌4例(7.14%)性爱在线破解版黄色网站;支氣管肺部炎癥病變37例(28.91%)国产av专区,其中30例經(jīng)防污毛刷采樣、支氣管肺泡灌洗液行細菌學培養(yǎng)亚洲中文字字幕精品乱码,病原菌的檢出率分別為50%(15例)和40%(12例)国产激情图区;肺結(jié)核12例(9.38%),其中合并支氣管內(nèi)膜結(jié)核3例(25%)欧美变态人XXXX,刷檢涂片和支氣管肺泡灌洗液找到結(jié)核桿菌分別為11例(91.67%)和5例(41.67%)日韩欧美精品视频在线观看,病理確診為結(jié)核性乾酪樣肉芽腫2例(16.67%);特發(fā)性肺間質(zhì)纖維化2例(1.56%)老板轻点好痛好涨嗯啊视频,肺淋巴瘤1例(0.78%)超碰在线入口。
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In this thesis,the AM1,MNDO,MINDO/3(mainly AM1)and INDO/S-CI semiempirical MO methods were used toinvestigate the excited-state intramolecular protontransfer reactions of salicylic acid derivatives—salicylic acid,methyl salicylate,salicylaldehyde,o-hydroxyaceto-phenone,salicylamide and 3-hydroxy-picolinamide (6 conformers and 2-3 anion species);2-(2'-hydroxy-5' methylphenyl) benzotriazole(4 conformers),2-(2' hydroxyphenyl) benzimidazole (3 conformers and 3anion species),Bis-2,5-(2-benzoxazolyl)hydroquinone(3 conformers),2-(2'-hydroxyphenyl)benzothiazole(2conformers) and 7-azaindole dimer (2 conformers).Theinvestigations were described as follows.Geometry optimization,relative stability andhydrogen bonding energy First,for sylicylic acid derivative molecules,the AM1,MNDO and MINDO/3 methods were used toinvestigate ground-state geometry optimization,energies,relative stabilities and hydrogen-bondingenergies on the five kinds of the molecules(designing 6 conformers and 2-3 anion species).Comparing with experimental data,the optimizedgeometry,the order of stability,the hydrogen-bonding energies and the distances between O-O in O-H..O hydrogen bonds by AM1 method were in agreementwith the experimental data,however,the C-C bondlengths optimized by MNDO and MINDO/3 were longer,C-O and O-H bond lengths were shorter;for C-N bondlengths,the results opitimized by MNDO method werethe same as those by AM1 method,nevertheless the C-Nbond lengths given by MINDO/3 method were muchshorter.For some sylicylic acid derivatives(e.g.methyl salicylate,salicylamide),the order ofstabilities on the conformers given by MNDO andMINDO/3 methods were not in agreement with theexisting conformers deduced by experimental methods,and the hydrogen bonding energies calculated by MNDO.and MINDO/3 methods were smaller.Second,the studyon the other systems found that the optimizedgeometry of the proton-transfered product with INDOmethod could not be obtained,only could theoptimized geometry of reactant be obtained,and thecalculated hydrogen bonding energies were greater.Many results of calculation indicated that the studyon the excited-state intramolecular proton transferreaction system using AM1 method was suitable andreliable.
本論文用AM1、MNDO亚洲男人第一Av网站国产、MINDO/3(主要是AM1)和INDO/S-CI半經(jīng)驗分子軌道方法對水楊酸衍生物系列——水楊酸免费视频性综合、水楊酸甲酯欧美亚洲国产另类、水楊醛、O-羥基乙酰苯酮湖北一级黄片、水楊酰胺和3-羥基吡啶酰胺(6種異構(gòu)體和2-3種陰離子)欧美日韩经典自拍视频网站;2-(2'-羥基-5'-甲基苯基)苯并三〓唑(4種異構(gòu)體);2-(2'-羥基苯基)苯并咪唑(3種異構(gòu)體和3種陰離子)日韩AV电影三级片导航网站;2超91自拍偷拍,5-二間氮雜氧茚氫醌(3種異構(gòu)體);2-(2'-羥基苯基)間〓雜硫茚(2種異構(gòu)體)和7-〓吲哚二體(2種異構(gòu)體)的激發(fā)態(tài)分子內(nèi)質(zhì)子轉(zhuǎn)移反應在以下幾個方面進行了較系統(tǒng)的理論研究:幾何構(gòu)型優(yōu)化和相對穩(wěn)定性及氫鍵能首先以水楊酸衍生物系列分子為例国产精品女人高潮毛片,用AM191精品午夜在线看桃花岛、MNDO和MINDO/3方法考察了5種分子(每種分子設(shè)計6種異構(gòu)體和2-3種陰離子)的基態(tài)幾何構(gòu)型優(yōu)化,能量久久久影院国内精品、相對穩(wěn)定性和氫鍵能計算欧美精品专区视频免费在线观看,通過和實驗數(shù)據(jù)進行比較,AM1方法給出的優(yōu)化幾何構(gòu)型亚洲国产一级毛片、穩(wěn)定性次序国产多人群p在线视频、氫鍵能和O—H。强奸资源影音先锋。日韩人人操人人摸。O氫鍵的0—0距離與實驗數(shù)據(jù)吻合最好,MNDO和MINDO/3方法優(yōu)化的C-C鍵長偏長人人搞人人操人人叉一,C-O鍵和O-H鍵長偏短18岁禁污网站;對于C-N鍵長,MNDO和AM1優(yōu)化結(jié)果差別不大久射久aV,而MINDO/3給出了過短的C-N鍵長全国男人的天堂网,天堂网,MNDO和MINDO/3方法給出的有些水楊酸衍生物分子(如水楊酸甲酯和水楊酰胺)異構(gòu)體的穩(wěn)定性次序和實驗上推測的可存在異構(gòu)體結(jié)果不一致,MNDO和MINDO/3方法給出的氫鍵能偏低激情欧美自拍偷拍,對其他體系的研究發(fā)現(xiàn)INDO方法常常不能得到質(zhì)子轉(zhuǎn)移產(chǎn)物的優(yōu)化幾何構(gòu)型激情文学网,只能得到反應物的優(yōu)化構(gòu)型,并且估算的氫鍵能偏高插插黄色视频免费观看,大量的計算結(jié)果表明AM1方法對本論文研究的激發(fā)態(tài)分子內(nèi)質(zhì)子轉(zhuǎn)移反應體系是適宜和可靠的国模吧高清女模专业网站。
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[Objective]To research the method, curative effect and indications of applying arthroscopy to treat hip disease, and investigate the valuation of arthroscopy on hip disease.[Method]Totally 103 patients (129 hips) were treated with arthroscopy by corresponsive modus operandi according to different pathogenetic conditions from Oct.2001 to Jan. 2007. Among the total cases, 50 hips were osteonecrosis of the femoral head; 8 hips were traumatic hip arthritis; 34 hips were hip osteoarthritis; 9 hips were pigmented villonodular synovitis; 12 hips were hip arthritis of ankylosing spondylitis; 6 hips were chronic synovitis; 6 hips were acetabular labrum injury; 2 hips were hip joint corpus liberum; 2 hips were coxarthropathy with indefinite reasons.
[目的]研究髖關(guān)節(jié)鏡治療髖關(guān)節(jié)疾病的方法、療效及適應證你懂滴视频,探討關(guān)節(jié)鏡在髖關(guān)節(jié)疾病中的臨床價值[方法]2001年10月~2007年1月黄色视频在线网站2,采用髖關(guān)節(jié)鏡手術(shù)治療103例(129髖)髖關(guān)節(jié)疾病,其中股骨頭壞死50髖你懂的偷拍;創(chuàng)傷性髖關(guān)節(jié)炎8髖色国产精品福利;髖關(guān)節(jié)骨性關(guān)節(jié)炎34髖在线大片aaa;色素沉著絨毛結(jié)節(jié)性滑膜炎9髖992tv国产精品;強脊性髖關(guān)節(jié)炎12髖;慢性滑膜炎6髖;髖關(guān)節(jié)盂唇損傷6髖国产美女插逼视频;髖關(guān)節(jié)游離體2髖://www.gdmyzz.com/;不明原因髖痛2髖。
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Twelve tumors were solid and three contained cystic components. Nine were inhomogeneously hypoechoic. Two were homogeneously hypoechoic. Two were inhomogeneously isoechoic. Two were homogeneously isoechoic. Two contained microcalcification. One with acoustic shadowing. Twelve were well-defined and three were ill-defined. Fourteen were regular and one was irregular. Four had acoustic halo. Six were ecstatic. Nine were not ecstatic. Thirteen were examined with color Doppler ultrasound. Nine had abundent branched blood flow within the tumor and ring blood flow around. Two had short linear blood flow within the tumor and ring flow around.
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Objective To analyze the CT and MRI manifestations of oral carcinoma,and to evaluate the value of imaging technologies in the diagnosis of oral carcinoma,thus to explore the most reasonable imaging method Methods 46 cases with oral carcinoma,proved by surgery and pathology (2 lip tumors,8 carcinomas of the gingival ridge,5 carcinomas of the buccal mucosa,24 tongue carcinomas,5 carcinomas of the floor of the mouth,2 tumors of the hard palate),were analysed retrospectively32 cases were examined with CT and 14 cases with MRIImaging presentations were compared with surgery and pathology Results Of the 46 cases with oral carcinoma,22 were ulcerative lesion of infiltrative growth,24 were masslike lesion of extruded trowthOf the 32 cases examined with CT scan,27 were detected,5 were missed,the 4 missed cases were confirmed to be superficial ulcers by surgery and pathologyOf the 10 cases with bone destruction examined with CT scan,8 were manifested,2 were missedAnd of the 14 cases with MRI scan,all 14 were detected Conclusion CT and MRI scan are valuable for diagnosis of oral carcinomasThey can manifest the morphology and size of primary tumor,contiguous structures,bone invasion and cervical lymph node metastasisMRI is the optimal imaging method for superficial ulcerative lesion of infiltratibe growth
徐 鋒,羅德紅十八级毛片在线观看99ri,唐威另类亚洲欧美专区第一页,歐陽漢,周純武目的分析口腔癌的CT及MRI表現(xiàn)日韩在视频,評價影像檢查在口腔癌診斷中的應用價值亚洲国产欧美日韩欧美2016,探索合理的影像學檢查方法。方法回顧性分析經(jīng)手術(shù)及病理證實的口腔癌46例(唇癌2例黄色页一级在线观看,牙齦癌8例久久免费视频性爱视频,頰黏膜癌5例,舌癌24例精品163黄色录像,口底癌5例国产污污网站入口,硬腭癌2例),其中32例行CT掃描欧美视频在线观看免费电影,14例行MRI掃描亚洲欧美在线观看wwwwww,將影像表現(xiàn)與手術(shù)所見及病理結(jié)果進行對照分析美国黄片一级A片。結(jié)果 46例口腔癌中,呈浸潤性生長的潰瘍型病變22例国产野视频在线观看快播,外生性生長的腫物型病變24例红牛影视AV在线地址。CT掃描32例,明確顯示病變27例精品国内在线,遺漏病變5例囯产激清黄色网,其中4例為表淺潰瘍型病變。CT掃描病例中精品欧美一区二区在线观看视频性色,10例有骨質(zhì)侵犯久久青草精品同性恋视频,CT掃描準確評價8例,遺漏2例最近中文字幕免费大全下载。MRI掃描14例久久久久久久精品国产免,均準確評價腫瘤及其與周圍結(jié)構(gòu)的關(guān)系。結(jié)論 CT国产综合男女视频一区二区、MRI對口腔癌原發(fā)腫瘤的形態(tài)www,3rb3rb免费视频、大小、周圍結(jié)構(gòu)侵犯日韩一区黄色小视频、頸部淋巴結(jié)轉(zhuǎn)移的評價有重要價值激情按摩系列片AAAA国产馆。對于較小及呈浸潤性生長的表淺潰瘍型腫瘤,CT掃描尤其是CT平掃難以準確顯示日韩-本道黄色网站站,應首選MRI国产一区在。
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Objective To analyze the CT and MRI manifestations of oral carcinoma,and to evaluate the value of imaging technologies in the diagnosis of oral carcinoma,thus to explore the most reasonable imaging method Methods 46 cases with oral carcinoma,proved by surgery and pathology (2 lip tumors,8 carcinomas of the gingival ridge,5 carcinomas of the buccal mucosa,24 tongue carcinomas,5 carcinomas of the floor of the mouth,2 tumors of the hard palate),were analysed retrospectively32 cases were examined with CT and 14 cases with MRIImaging presentations were compared with surgery and pathology Results Of the 46 cases with oral carcinoma,22 were ulcerative lesion of infiltrative growth,24 were masslike lesion of extruded trowthOf the 32 cases examined with CT scan,27 were detected,5 were missed,the 4 missed cases were confirmed to be superficial ulcers by surgery and pathologyOf the 10 cases with bone destruction examined with CT scan,8 were manifested,2 were missedAnd of the 14 cases with MRI scan,all 14 were detected Conclusion CT and MRI scan are valuable for diagnosis of oral carcinomasThey can manifest the morphology and size of primary tumor,contiguous structures,bone invasion and cervical lymph node metastasisMRI is the optimal imaging method for superficial ulcerative lesion of infiltratibe growth
中華碩博網(wǎng)核心提示:徐鋒,羅德紅久久免费AV片国产精品,唐威91久久久久精品久久久久久久久,歐陽漢,周純武關(guān)鍵詞:口腔腫瘤目的分析口腔癌的CT及MRI表現(xiàn)日本高清wwwcom,評價影像檢查在口腔癌診斷中的應用價值欧美亚洲日本高清不卡,探徐鋒,羅德紅欧美丰满熟妇性XXXX动图,唐威0808贵妃网理论片,歐陽漢,周純武目的分析口腔癌的CT及MRI表現(xiàn)啊啊啊用力插好舒服国产视频,評價影像檢查在口腔癌診斷中的應用價值天海翼精品久久久久中文字幕,探索合理的影像學檢查方法。方法回顧性分析經(jīng)手術(shù)及病理證實的口腔癌46例(唇癌2例一九九精品在线免费观看视频,牙齦癌8例偷拍外国呦呦呦,頰黏膜癌5例,舌癌24例91看片午夜在线影院,口底癌5例性交色片视频,硬腭癌2例)孕妇激情视频片,其中32例行CT掃描,14例行MRI掃描国产高潮动漫在线观看免费视频,將影像表現(xiàn)與手術(shù)所見及病理結(jié)果進行對照分析。結(jié)果46例口腔癌中欧美黄色网站在线看,呈浸潤性生長的潰瘍型病變22例日韩美女观看成人网站在线观看视频,外生性生長的腫物型病變24例。CT掃描32例你懂得视频网站,明確顯示病變27例久久国产免费一区二区三区,遺漏病變5例,其中4例為表淺潰瘍型病變一级一级a爱片免费免免。CT掃描病例中欧美黄片1区,2区,3区,10例有骨質(zhì)侵犯,CT掃描準確評價8例欧美日韩成年人色免费看片,遺漏2例一级黄色在线免费看。MRI掃描14例,均準確評價腫瘤及其與四周結(jié)構(gòu)的關(guān)系永久看欧洲大片。結(jié)論 CT欧美日韩A片网站在线看、MRI對口腔癌原發(fā)腫瘤的形態(tài)、大小亚洲熟女激情视频网、四周結(jié)構(gòu)侵犯影音先锋一区二区三区无码电影网、頸部淋巴結(jié)轉(zhuǎn)移的評價有重要價值。對于較小及呈浸潤性生長的表淺潰瘍型腫瘤国产精品黄色网,CT掃描尤其是CT平掃難以準確顯示欧美色欧美亚洲重口二区,應首選MRI。
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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.
為研制帶有O型口蹄疫病毒(Foot-and-Mouth Disease Virus国产乱论色网站,F(xiàn)MDV)China99株結(jié)構(gòu)蛋白基因及多個非結(jié)構(gòu)蛋白基因的DNA疫苗在线免费观看黄片av,本研究通過定點突變方法和PCR擴增方法,獲得包含有FMDV China99株結(jié)構(gòu)蛋白P1久久精品免费视频60-70、非結(jié)構(gòu)蛋白2A在线看片aa黄色视频、3C以及部分2B、3B編碼基因的片段P12X3C和包含有FMDV China99株結(jié)構(gòu)蛋白P1无码不卡乱人伦中文视频、非結(jié)構(gòu)蛋白2A特黄A级毛片免费网站、3C、3D以及部分2Bjizz免费在线国产、3B編碼基因的片段P12X3C3D女生的鸡鸡的黄色视频,將獲得的基因片段直接/酶切后與同樣處理的真核表達質(zhì)粒連接,分別得到重組質(zhì)粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D国内自拍网、pTARGET/P12X3C3D欧美一级偷拍视频;對重組質(zhì)粒進行序列測定午夜性大片免费视频、分析,并將重組質(zhì)粒分別轉(zhuǎn)染BHK-21細胞一级A片无码大黄片,通過雙抗體夾心ELISA方法和間接免疫熒光標記方法檢測細胞中FMDV抗原的表達色a电影网,用電子顯微鏡觀察病毒空衣殼的組裝;為評價重組質(zhì)粒作為DNA疫苗對實驗動物及本動物的免疫效果日韩人妻无码免费视频一二区色,將重組質(zhì)粒經(jīng)肌肉注射方法接種豚鼠精品人人做人人爽爱在碰,并與酵母表達的純化FMDV China99株3D蛋白及帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1/IFN分別/同時免疫,第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株www·C0m黄色视频;隨后將質(zhì)粒pcDNA3.1/P12X3C先锋影音在线资源站大全、pcDNA3.1/P12X3C3D與帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1/IFN同時免疫牛,三周后經(jīng)牛舌皮攻以10〓ID〓的O型FMDV China99株美女被艹色视频免费看。
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By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.
為研制帶有O型口蹄疫病毒(Foot-and-Mouth Disease Virus一a网大全在线,F(xiàn)MDV)China99株結(jié)構(gòu)蛋白基因及多個非結(jié)構(gòu)蛋白基因的DNA疫曲,本研究通過定點突變方法和PCR擴增方法欧美一区二区操逼,獲得包含有FMDV China99株結(jié)構(gòu)蛋白P1欧美日韩第一区久久、非結(jié)構(gòu)蛋白2A、3C以及部分2B日韩欧美亚洲综合久久图片、3B編碼基因的片段P12X3C和包含有FMDV China99株結(jié)構(gòu)蛋白P1欧美性爱国产、非結(jié)構(gòu)蛋白2A、3C日本AAA片、3D以及部分2B亚洲人14个视频、3B編碼基因的片段P12X3C3D,將獲得的基因片段直接/酶切后與同樣處理的真核表達質(zhì)粒連接毛片无码高清,分別得到重組質(zhì)粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D2018日本高清色视频、pTARGET/P12X3C3D;對重組質(zhì)粒進行序列測定日韩高清在线一区二区三区、分析在线日韩日本国产亚洲,并將重組質(zhì)粒分別轉(zhuǎn)染BHK-21細胞,通過雙抗體夾心ELISA方法和間接免疫熒光標記方法檢測細胞中FMDV抗原的表達色一级A片视频在线播放网站大全,用電子顯微鏡觀察病毒空衣殼的組裝欧美大胆A级视频69;為評價重組質(zhì)粒作為DNA疫苗對實驗動物及本動物的免疫效果,將重組質(zhì)粒經(jīng)肌肉注射方法接種豚鼠欧美性爱乱,并與酵母表達的純化FMDV China99株3D蛋白及帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1/IFN分別/同時免疫日韩亚洲性爱A片,第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株:隨后將質(zhì)粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D與帶有牛α干擾素的真核表達質(zhì)粒pcDNA3.1/IFN同時免疫牛卡通动漫亚洲专区第一页,三周后經(jīng)牛舌皮攻以10~4ID_(50)的O型FMDV China99株无码毛片高清网站。
- 相關(guān)中文對照歌詞
- If You Were My Girl
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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.
然而,正如其名字所指出的那樣,CD盤不能寫小黄片真人视频毛片,也不能用任何方式改變其內(nèi)容v在线观看欧美精品。
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Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.
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A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.
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