Temporal and spatial expression pattern of gibel carp pou2 during embryogenesis was investigated by RT-PCR and whole-mount in situ hybridization. RT-PCR result indicates that there is maternal transcript of gibel carp pou2 in early embryos and the zygotic pou2 transcript is abundantly expressed from blastula stage. Large amount of pou2 transcript is detected at both 50% and 90% epiboly stages. At 100% epiboly stage, however, the pou2 transcript reduces rapidly and disappears at the later embryogenesis. Whole-mount in situ hybridization shows that the maternal transcript ubiquitously exists in all blastomere cells.

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The film or movie is the most popular and common video resource. In this paper we use the film video as an example, aims to implement real-time retrieve of explosion and shot scenes, has carried on the following research mainly: first, we proposed a method of real-time recognition of explosions based on dominate-color and audio information and improved the accuracy the retrieve;second, we proposed a method of real-time retrieve of shots scenes based on audio information and differences between frames when the shot occurs;third,a whole video abstraction system is proposed, it can cut off the clips which have been retrieved and merge them into a independent video abstraction in the same time edit the information of the it ;forth, it offers two-level retrieval structure of"key frame-highlights"and can play segmentation result and the whole video just like a video player.

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I combined 2 recipes, 100% Whole Wheat with Olive Oil and 100% Whole Wheat with Flaxseed.

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