Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭質粒YCEp1為載體構建西方許旺酵母部分基因組文庫亚洲黄色高清视频在线观看，在大腸桿菌中擴增后提取混合質粒DNA欧美,性爱精品亚洲性爱免费观看视，經電轉化非缺陷標志酒精酵母 AS.2.1364国产精品h片在线播放，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力篩選含水解淀粉的陽性轉化子，從陽性轉化子中分離重組質粒證實含5.0kb的插入片段日韩欧美国产一区呦呦，用α-淀粉酶基因兩端序列設計的引物PCR擴增及擴增片段序列分析證實該片段中含有α-淀粉酶全部編碼序列国产精品无码AⅤ片。

To study the applicable prospect of suicide gene on tumor therapy in clinic,we cloned the gene of D-amino acid oxidase and the gene of yeast cytosine deaminase.We hope to establish the transgenic mices of DAAO gene and YCD gene,and the two transgenic strain mices are valuable animal system for studying the biological characteristic of DAAO gene and YCD gene ,for studing the killing activity of DAAO/D-Ala and YCD/5-FC gene therapy systems on tumor.The D-amino acid oxidase gene derived from R.gracilis and it could oxidize D-amino acids. Hydrogen peroxide(H2O2) is a reactive oxygen species generated in the deamination of D-Ala catalyzed by DAAO.

為了探討自殺基因在臨床腫瘤治療中的應用前景，建立自殺基因腫瘤治療評價的動物模型在线午夜视频，我們克隆了D-氨基酸氧化酶(D-amino acid oxidase久久精品首页，DAAO)基因和酵母菌胞嘧啶脫氨酶(yeast cytosine deaminase，YCD)基因自拍偷拍ww，希望建立DAAO基因及YCD基因轉基因小鼠中国a片黄色录像，為研究DAAO及YCD基因的生物學特性、開發(fā)和評價DAAO／D-Ala及YCD／5-FC自殺基因系統(tǒng)進行腫瘤治療建立良好的實驗動物模型AAA美国三级黄片。

Dissolve the yeast in warm water and let it stand until foamy, about 3 to 5 minutes.

將yeast 溶在溫水中在线视频一区亚洲，直到很多小泡泡出來，大約3到5分鐘男女性激烈无遮掩免费观看。

Among the carbon sources examined, brown sugar and malt extract were best for the mycelial growth and the polysaccharide production. Besides, yeast extract and skim milk were the effective nitrogen sources.

在所測試的數種碳源中九九色无码精品网，以brown sugar和malt extract對菌絲生長及多醣生產效果較佳。yeast extract 和 skim milk為較佳之氮源一区二区无码电影天堂在线观看。

A semivowel produced with the tongue near the palate (like the initial sound in the English word 'yeast').

舌頭位于上腭附近發(fā)出的半元音（象英語單詞"yeast"中的首音国产精品iAGO视频网免费播放。

In model organism fission yeast,Schizosaccharomyces pombe,100-200 rDNA gene copies are located on both left and right ends of chromosomeⅢ.The integrity of rDNA is critical for cell growth and survival.

在模式生物裂殖酵母(fission yeast，Schizosaccharomyces pombe)基因組中欧美一区二区精品视频中国，100-200拷貝的rDNA基因分布在Ⅲ號染色體左右兩端的末端附近欧美VA日本一区。rDNA區(qū)的完整性對于細胞生長和存活是至關重要的。

This strain, grown on Thermus medium containing tryptone and yeast extract at 55℃ could produce protease.

將此菌株在添加tryptone 和yeast extract 的Thermus 基本培養(yǎng)基中一级毛片一级毛，於55℃下培養(yǎng)久久精品国产精国，會產生外泌性蛋白脢。

According to the above results, feather, tryptone, yeast extract as well as incubation time and temperature were selected for further studies by RSM. Data obtained showed the maximal biomass production and keratinase activity occurred at above 96 h.

根據上述結果選擇feather欧美黑人又粗又长精品、tryptone高端自拍无码在线、yeast extract外加兩個物理因子進行第一次的RSM分析，結果發(fā)現欲得到較高的角蛋白酶活性與菌體產量色污网站网站，其培養(yǎng)時間需超過四天以上亚洲精品国精品91。

Nine medium compositions were used to study their effect on I40 using Plackett-Burman design. The production of biomass for I40 was affected in order of yeast extract, tryptone, glucose, and production of protease affected in order of feather, tryptone and yeast extract.

最是化條件探討方面，於基礎培養(yǎng)基中分別添加九種單因子自拍偷拍精品专区，以PB design分析999A片免费看在线播放，結果發(fā)現對於I40生長影響較為顯著的因子依序為：yeast extract、tryptone噜噜性爱、glucose女生按摩破处视频在线观看，而對於該菌生產蛋白酶產量影響較為顯著的因子依序為：feather、tryptone99一级屁屁毛片、yeast extract亚洲一区二区无字幕。

From the commercial consideration, the incubation time was fixed at 3 days and reinvestigated by RSM. The maximal keratinase activity was obtained from the following condition: tryptone 0.16%,yeast extract 0.27%, feather 0.08%, incubation temperature 51.7С.

由於長時間培養(yǎng)容易造成污染在线免费黄站，且不具經濟效應，因此將反應時間調整為3天日韩国产欧美亚洲，進行第二次的RSM分析午夜理论在线，得到之培養(yǎng)條件為：tryptone 0.16 %， yeast extract 0.27 %乱中年熟女伦av一区， feather 0.08 %及 temperature 51.7С一级黄色一级。