Olive oil, tributyrin and p-nitrophenyl palmitate were used as substrates to measure lipase activity.

分別以橄欖油一级视频在线观看免费播放、三丁酸甘油酯和對硝基苯酚棕櫚酸酯為底物檢測展示的脂肪酶酶活。

Eight lipase-producing bacterium strains were screened from 40 cold-adapted strains, these strains isolated from the mud of Parece Vela sea basin in the Pacific Ocean of 5010m deep, of which two similar strains have the highest lipolytic activity and are positive to olive oil.

通過三丁酸甘油酯平板法從太平洋帕里西維拉海盆5010m的底泥中共篩出八株可產(chǎn)脂肪酶的菌株，其中的兩株生理生化特征極其相近的菌脂肪酶活性最高，并且對橄欖油平板產(chǎn)生熒光6080午夜视频在线观看。

Objective To investigate the differentiation of NB4 and MR2 cells induced by tributyrin in combination with different doses of all-trans retinoic acid and to see if TB can induce apoptosis of NB4 and MR2 cells.

目的 研究三丁酸甘油酯與全反式維甲酸不同濃度組合對NB4及MR2細胞的誘導(dǎo)分化作用，并觀察TB對NB4日韩欧美综合在线、MR2細胞是否具有促凋亡作用。

The most common methods used for screening of lipase-producing strains are observation of transparent circle on tributyrin agar plate and color-changing circle on olive oil agar plate.

在脂肪酶產(chǎn)生菌的篩選中自拍91，最常用的方法是三丁酸甘油酯瓊脂平板透明圈法和橄欖油瓊脂平板變色圈法AAA级黄片。

However, the recombinant yeast could not grow on the traditional tributyrin plate because this kind of medium is lack of essential nutrients for yeast growth.

而傳統(tǒng)的三丁酸甘油酯平板缺乏有效營養(yǎng)成分，重組酵母無法生長日本中文字幕的视频网站；橄欖油平板靈敏度較低A女人黄色网站，低脂肪酶活力的重組酵母不能產(chǎn)生變色圈，這給產(chǎn)脂肪酶基因工程菌的鑒定帶來了困難卡通动漫亚洲图片。

We use plate to check the lipase activity ,the lipase activity is very low, Compared to the wild type , the mutant could not form the clear halo on the olive oil plate but tributyrin plate, it showed that the 227 th Serine was essential for the lipase activity.

與野生型相比91九色国产，突變體脂肪酶的酶活很低，不能在橄欖油平板上形成透明圈黄色性片视频免费公开，但可以在以三丁酸甘油酯為底物的平板上形成透明圈久久久在精品视频，表明第227位絲氨酸可能對脂肪酶酶酶活具有重要影響。

To elucidate the possible mechanism of differentiation and /or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor, the level of acetylated histone H3 was detected by Western blot, p21~ WAF1expression was detected by semi-quantitative RT-PCR.

為了探討組蛋白去乙?8岁禁止黄色；敢种苿?strong>三丁酸甘油酯(tributyrin女人黄图视频图片，TB)誘導(dǎo)NB4、K562白血病細胞分化和凋亡的作用機制国产一区二区性交影视，利用Westernblot方法及逆轉(zhuǎn)錄聚合酶鏈反應(yīng)檢測TB作用前后NB4在线观看国产爆草视频网站免费、K562細胞組蛋白H3乙酰化水平以及p21WAF1表達量的改變日韩福利免费网站。

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

為了探討組蛋白去乙蹙匪客喙栽谙吲腁V一区；敢种苿?strong>三丁酸甘油酯(tributyrin， TB)誘導(dǎo)NB4夜夜夜综合亚洲伊人、K562白血病細胞分化和凋亡的作用機制亚洲黄色网站在线观看免费，利用Western blot方法及逆轉(zhuǎn)錄聚合酶鏈反應(yīng)檢測TB作用前后NB4、K562細胞組蛋白H3乙趺琅凡賐；揭约皃21(上標 WAF1)表達量的改變久久精影院。

The activity of lipasc from S78- pVT102U/a -lip was up to 20U/ml after 72h fermentation; The product of S78-pVT102U/a-L-lip07 was little, only formed the halo on the tributyrin plate.

其中，重組酵母S78-pVT102U／α-lip搖瓶發(fā)酵72h狼友视频—主页，酶活可達20U／ml免费观看黄色网页；S78-pVT102U／α-L-lip07表達的脂肪酶很少国产成熟美女三级视频直接看，表達產(chǎn)物僅在三丁酸甘油酯平板上形成透明圈。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

該菌株經(jīng)IPTG誘導(dǎo)可表達分子量約80 kDa的融合蛋白免看一级a一片免费，SDS-PAGE分析表明融合蛋白位于菌體超聲裂解后的上清中日本和亚洲黄色视频多毛水视频，用蛋白純化試劑盒HisTrap~ HP純化后得到單一的目的蛋白，約80 kDa欧美三级一级视频，且純化的融合蛋白具有脂酶活性国产性色Aⅴ综合一，可以分解三丁酸甘油酯。