A new method using gel permeation chromatographic cleanup followed by high performance liquid chromatography-tandem mass spectrometry has been established for quantitative determination of Maleic hydrazide,Prowl and butralin residues in tobacco.

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In order to overcome the shortage of conformable precision, polyamide affinity membrane modified by cibacron blue F3GA was introduced into the pretreatment unit to get rid of interferer from large molecule such as albumin in serum. The affinity membrane was combined with micro-fluidic chip by embedded and external methods and using external-combination pretreatment unit, average enrichment was 89.36 times, RSD was 4.37%.

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Vesic's solutions to cavity expansion that include cylindrical cavity expansion and spherical cavity expansion are introduced. Then cylindrical cavity expansion theory is applied to study compaction effect of pile driving in saturated soil. Based on the results of conventional triaxial tests, a trilinear curve model is used to simulate stress-strain curve of strain-softening materials. Meanwhile, trilinear curves are also used to simulate curves of volumetric strain, minor principal strain and major principal strain. By using elasto-plastic theory, analytical solutions to cylindrical cavity expansion in strain-softening soil are presented. According to the mechanism of statically pressed pile driving, compaction effect of pile tip can be viewed as hemispheric cavity expansion. Therefore spherical cavity expansion theory is applied to obtain the solutions to stress, strain, displacement fields and final pressure. Resistance force of pile tip is calculated to estimate static pressure.

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Working conditions of drilling string is complex underground, various factors ofteninteract, in the process of drilling, drilling string acts complex movement in drill fluid, drillfluid unavoidable corrupt drilling string because such as oxygen, hydrogen sulfide and manysalts are dissolved in it. At the same time, many cyclic stresses act on drilling pipe, such asflexural cyclic stress, torsional cyclic stress.

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objective to isolate and purify the antimicrobial peptides with anti-toxoplasma gondii activity isolated from the haemlymph of musca domestica larvae.methods the antimicrobial peptides of musca domestica larvae were induced by infection and injury were isolated and purified by trituration,centrifugalization and column chromatography.then the antimicrobial peptides with anti-toxoplasma activity were sieved by mtt colorimetric method and haemacytometry.results it was found that two antimicrobial peptides had anti-toxoplasma activity through resource s cationic column chromatography and superdex g75 gel column chromatography.conclusion the two antimicrobial peptides with anti-toxoplasma activity existing in the haemlymph of musca domestica larvae had different anti-toxoplasma effect.

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The soluble peptidoglycan is gained by short dose benzylpenicillin sodium and sephadex G-100; the SLP reagent kit revealed the prepared soluble peptidoglycan is peptidoglycan; the limulus test showed that the content of LPS in the prepared soluble peptidoglycan(10ng/ml) is lower (0.01ng/ml); the improved LOWRY reagent kit showed the content of protein in the prepared soluble peptidoglycan(1.0mg/ml) was 0.06mg/ml; the optimization condition is 0.75mg/ml of the dosage of benzylpenicillin sodium,1h of the incubation time of benzylpenicillin sodium addition and 5×108CFU/ml of the bacterial concentration.

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Nepalensis wall polysaccharide was prepared with hot water extraction,ethanol precipitation,dialyzation,and lyophilization.the crude polysaccharide was separated by deae-cellulose chromatography and purified by sephadex g-200 chromatography.the inhibit effect of the polysaccharide on lipid peroxidation of mice liver was conducted by tba method.the model of liver-injury induced by carbon tetrachloride（ccl4was used to validate the variety of the realated ast.results onefold ingredient polysaccharide was obtained after isolated and purified.conclusion the ingredient can remarkably inhibit lipid peroxidation of rat liver and significantly reduce activity of ast.

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Reaction is carried out under a fractionating column that allows aldehyde to pass but returns alcohol to the reaction vessel.

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The Cretaceous sedimentary rocks were baked by the basaltic lava flow erupted in the Early Miocene, contracting in volume owing to loss of water contained mainly in the clays of the clastic rocks, and thus forming the columnar jointing. According to the occurrences of jointing and the sedimentary strata the authors calculated the two different rotations of the Mellit block: the block tilted toward 338° for 18° from Late Cretaceous to the beginning of Miocene, and tilted toward 138° for 18° since Miocene.

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Methods Rhizoma Gastrodiae, Pellis Ovis, and Calculus Bovis Sativus in Tianhuang Houzao powder were identified by TLC. The content of Gastrodin in the powder was determined by HPLC. The HPLC condition was as follows: column was Phenomenex C18 (4.60 mm×250 mm, 4 m), the mobile phase consisted of methanol-0.05% phosphoric acid (3:97), the UV detection wavelength was at 220 nm, the flow rate was 1.0 ml/min with column temperature at 25℃.

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