Morphological and flourescent changes were assessed using confocal microscopy in whole-mount organ of Corti preparations. Results (1) After the animals were exposed to broadband noise at 122 dB SPL in 4 h/day for 2 days, both apoptosis and necrosis appeared in OHCs. The single strand DNA in apoptotic OHCs were observed both in guinea pigs and mice.(2) In normal OHCs, EndoG was distributed outside of nuclei. EndoG translocated from outside to inside of the nuclei in both apoptotic and necrotic OHCs following noise exposure.(3) The MNNG cochlear perfusion and noise exposure both caused the transloctation of AIF from the mitochondria to the nuclei. The translocation of AIF took place in both apoptotic and necrotic OHCs.

結果 (1)暴露于120 dB SPL的白噪聲環(huán)境中每天4小時，連續(xù)2天后引起豚鼠和小鼠耳蝸外毛細胞凋亡時黄片在线看。，其細胞核內產生ssDNA欧美一级作爱片，而在正常細胞內沒有三ssDNA；（2）在正常情況下无毒国产水多视频在线，EndoG分布于耳蝸毛細胞的細胞核外久久亚洲精品无码福利播放，在暴露于上述噪聲后發(fā)生凋亡和壞死的豚鼠耳蝸外毛細胞中，EndoG從細胞核外轉移到細胞核內国产白嫩在线观看视频，細胞核中的EndoG顯著增加乱伦网站97；（3）豚鼠耳蝸外淋巴灌流烷化劑MNNG后發(fā)生耳蝸外毛細胞凋亡和壞死，在凋亡和壞死的耳蝸外毛細胞中欧美三级片又粗又长，AIF自線粒體轉移到細胞核99re视频这里只有精品在，其變化與噪聲損傷引起耳蝸外毛細胞凋亡和壞死時一致。

First we first establish the apoptosis of related protein glossary database, then downloads the neurodegenerative disorders of apoptosis related abstract from the on-line biomedicine literature , and searchesfor the protein information database of the neurodegenerative disorders of apoptosis related pathway, then uses the data mining technology,with apoptosis of protein related glossary database use already establishment. Automatically searches for the neurodegenerative disorders of apoptosis related protein by the program in the biomedicine literature abstract separately in,(1) a sentence,(2) two sentences,(3) aliterature, finding simultaneously appears protein-protein interation. And comparing to the neurodegenerative disorders of apoptosis related protein database, looking for their repetition. This result comparing to the known neurodegenerative disorders of apoptosis related pathway, thus looking for unestablished neurodegenerative disorders of apoptosis pathway.

首先我們先建立細胞凋亡訊息蛋白質相關詞匯字庫国内偷拍人妻，再從線上生物醫(yī)學文獻下載神經退化性疾病細胞凋亡相關文獻摘要人人爱人人摸2020人人操，并且搜尋線上蛋白質資料庫中神經退化性疾病之細胞凋亡訊息蛋白質傳遞路徑，接著利用文獻探勘技術jizzzzzzz国产，使用已建立之細胞凋亡訊息蛋白質相關詞匯字庫亚洲人妻性爱片，以程式自動在生物醫(yī)學文獻摘要中搜尋神經退化性疾病細胞凋亡相關蛋白質分別在，（1）一個句子AAA级片免费小视频、（2）二個句子日韩欧美色色色、（3）一篇文獻，中同時出現的兩兩蛋白質關系黄色在线免费交配视频，再與已知線上神經退化性疾病細胞凋亡訊息傳遞路徑資料庫的蛋白質兩兩關系做比較黄色一级毛片在线观看，找出兩者重復建立的部分，把這個結果與線上已知之細胞凋亡訊息傳遞路徑資料庫蛋白質關系作比較男人的视屏超碰在线，由此找出尚未建立之細胞凋亡訊息傳遞路徑圖幸福宝网站最新入口。

And there were no evidence changes in histopathologic analysis of brain tissues in other ETU-injected and in control rat embryos. 2. Results of In situ cell apoptosis detection In E20 cerebral cortex, more Tunel cells were found in encephalocoele group than control group,and the results were significant.

二、應用細胞凋亡原位檢測法檢測不同組E20胎鼠大腦皮質中細胞凋亡情況 E20胎鼠大腦皮質中Tunel陽性細胞數目在正常組日韩欧美女自拍图片大全集高清视频、給藥無畸形組及脊柱裂組細胞凋亡數無明顯改變私库AV操一操人人操，而在腦膨出組胎鼠大腦皮質細胞凋亡數明顯多于對照組，計算凋亡細胞百分率免费无码动漫AⅤ片在线观看，然后用X~2檢驗進行分析欧美操逼视频看看，腦膨出組和對照組的細胞凋亡指數差異有統(tǒng)計學意義(P.05)。

The expression changes of relating-apoptosis gene proteins (bcl-2, bax) were detected by immunohistochemistry before and after treating with the Agaricus Blazei Murill extract to explore the possible mechanisms of inducing apoptosis for MGC-803 cells in vitro. Results: The Agaricus Blazei Murill extract significantly inhibited the proliferation of gastric cancer cells in vitro and the inhibitive effects were depended on the medicine concentration and treating times. After treating 24 hours on the gastric cancer cells with the morphologic changes of apoptosis with chromatin margination, karyopyknosis, karyorrhexis were found by the light.

結果：(1)通過細胞培養(yǎng)和MTT法精品一级毛片视频，表明長白山姬松茸在體外對MGC-803細胞株有明顯的抑制作用探花自拍日韩视频在线播放网站，加藥組與對照組相比其生長抑制率有顯著性差異(P＜0.01)国产精品丝袜，而且這種抑制作用呈現濃度和時間的依賴性；(2)通過集落形成率的測定結果表明日韩视频一区，加藥組與對照組相比其集落形成率和集落形成抑制率有明顯差異(P＜0.01)操学生妹视频，說明長白山姬松茸對MGC-803胃癌細胞株有明顯抗增殖作用；(3)光鏡觀察結果表明操操操射射，加藥組可見細胞脫水濃縮伊紅染色增強好黄色网站，胞體縮小，內含高度濃縮的胞核呈深藍色等典型細胞凋亡形態(tài)三级黄色网站在线观看；經AO／EB熒光染色觀察結果表明插进入视频，當終濃度為1.0mg／ml的姬松茸提取物作用于MGC-803胃癌細胞24h后，其凋亡率和破膜率與自然凋亡率與破膜率相比性爱视频在线免费观看，均有顯著性差異91影久久久，其凋亡率86.3％(P＜0.001)，破膜率為41.6％(P＜0.01)www.中文字幕.com，說明姬松茸確實有誘導MGC-803胃癌細胞凋亡的作用亚洲最大黄色网站在线观看视频在线观看免费，同時也有細胞毒作用，但以誘導細胞凋亡為主欧洲精品精美色视频在线观看；(4)免疫組化結果表明小黄片女生丝袜视频，用藥前后凋亡相關基因的BCl-2、Bax蛋白均有顯著性的改變(P＜0.001)印度高清AV。

Usually, anti-apoptotic protein is insulated in organella, restraining the release of pro-apoptotic factor and controlling cell apoptosis. however, interacting with pro-apoptotic protein, it can not restrain apoptosis, induce the loss of mitochondrial function, accelerate the release of pro-apoptotic factor and result in apoptosis.

抑凋亡蛋白平時被隔離在線粒體等細胞器內抑制促凋亡因子的釋放欧美性爱日，具有抑制細胞凋亡的功能，但一旦與激活的促凋亡蛋白發(fā)生相互作用后自拍视频网，便喪失了對細胞凋亡的抑制作用色哟哟最新网站入口，造成線粒體等細胞器的功能喪失和細胞器內促凋亡因子的釋放，導致細胞凋亡日韩侵犯精品视频在线播放。

Anti-apoptotic and pro-apoptotic factors have synergistic effect and play a switch role. Usually, anti-apoptotic protein is insulated in organella, restraining the release of pro-apoptotic factor and controlling cell apoptosis.

抑凋亡蛋白平時被隔離在線粒體等細胞器內抑制促凋亡因子的釋放国产真人一级做a片，具有抑制細胞凋亡的功能国产饥渴一区在线，但一旦與激活的促凋亡蛋白發(fā)生相互作用后天天爱天天做久久狠狠，便喪失了對細胞凋亡的抑制作用，造成線粒體等細胞器的功能喪失和細胞器內促凋亡因子的釋放亚洲欧美天堂综合久久，導致細胞凋亡久久亚洲人成综合网。

The results indicated that the typical apoptosis was induced by PRRSV in lungs and uteri. The ultrastructural changes showed different characterizations at different stages of the infection. During the early stage from 8th h to day 3 postinfection,the apoptosis cells shrank,the cytoplasm concentrated,the chromatin condensed,the kernel disaggregated and the endoplasmic reticulum dilated. During the middle stage from day 5 to day 9 postinfection,the apoptosis cells evidently shrank,the cell membranes protu berated,the plastosome manifolded,and apoptosis bodies were found. During the last stage from day 10 upwards postinfection,the apoptosis bodies degenerated and disappeared,and no inflammation occurred. Few apoptosis cells were necrotic.

結果表明，PRRSV可誘導宿主肺和子宮發(fā)生典型的細胞凋亡大黑吊日逼，表現為細胞的超微形態(tài)結構隨著病毒感染進程出現不同的特征性變化国产黄色网站在线，早期（感染后第8 h至第3 d）凋亡細胞多表現為細胞體積縮小，細胞質密度增強黄色在线视频免费，染色質濃縮日韩国产播放不卡，核仁解體久操精品在线，內質網擴張；中期（感染后第5 d至第9 d）凋亡細胞體積顯著縮小操操操com，細胞膜突起亚洲激情图库专区，線粒體增多，有凋亡小體形成人体国模私拍av；晚期（感染后第10 d以后）凋亡細胞形態(tài)多表現為凋亡小體降解和消失欧美日韩一区二区三区在线观看，少數凋亡細胞在凋亡晚期表現為壞死細胞。

The iso-electric point of was 4.8-5.2. The amino acid analysis indicated that the recombinant protein is non- N-glycosylated.The ability of 0.5 ug/m rhsTRAIL to induce apoptosis of liver cancer cells SMMC7721 and BEL 7402 could be visioned morphologically. This was further verified by Electrical microscope observision of induced SMMC7721 cells and agarose gel electrophoresis of apoptosis DNA. MTT test results also showed that 0.01 u g/ml purified rhsTRAIL has strong effect to induce apoptosis of SMMC7721 cell.

形態(tài)學上觀察了rhsTRAIL對肝癌細胞SMMC7721和BEL7402的誘導凋亡活性亚洲综合二区，發(fā)現rhsTRAIL在0.5μg/ml時顯示了很好的誘導凋亡活性日韩AA黄片，并通過電鏡觀察rhsTRAIL誘導后的SMMC7721細胞，證實了凋亡国产日韩精品AⅤ一区二区；同時提取凋亡細胞DNA99re5久久在热线播放，進行瓊脂糖電泳分析，在分子水平證明凋亡欧美一区2区三区3区公司；最后利用SMMC7721細胞分組加入純化后的rhsTRAIL蛋白在线观看国产综合日韩视频，結果顯示純化蛋白含量在0.01μg/ml時細胞就呈現了誘導凋亡活性。

XIAP expression could not be affected by the apoptotie inductions mediated by death receptor and mitochondria pathway in both cell lines.

通過對腎癌組織和細胞的研究一区二区色，提示抗凋亡的XIAP在腎癌組織中呈腫瘤進展依賴性增高偷拍精品日韩视频、在抗凋亡的腎癌細胞高表達，在腎癌細胞抗凋亡機制中發(fā)揮重要的作用av毛片在线看，影響腎癌對凋亡信號的敏感性毛片免费地址，使腎癌細胞獲得生存發(fā)展的優(yōu)勢，從而促進腎癌的進展青青女在线被干视频，并產生對誘導凋亡藥物的抵抗www.jizzcom；應用基因克隆和Tet-Off基因可控性表達系統(tǒng)技術構建的腎癌細胞XIAP穩(wěn)定表達細胞系，對深入研究XIAP在腎癌抗凋亡中的機制色先锋先锋影音AV资源站，具有實用意義3级电影网站入口。

1、Cur inhibits K562 cells growth and induces cell apoptosis may be correlated with the down-regulation of p210~亚洲视频在线观看第五区、inhibition of protein tyrosine phosphorylation and the signaling molecules such as p-Erk1/2看一下最好最好的黄片片最刺激的视频、c-myc which are relevant with cell growth and apoptosis; 2、Cur synergizes STI571 to inhibit K562 cell growth and induce cell apoptosis may be correlated with the down-regulation of p210~久久老司机亚洲精品福利网站、inhibition of protein tyrosine phosphorylation and the signaling molecules such as Hsp90久久精品无码专区免费东京热、PKC which are relevant with cell growth and apoptosis; 3、Cur reverses the resistance of K562/G01 cells to STI571, and synergizes STI571 to inhibit K562/G01 cell growth and induce cell apoptosis; 4欧美一级aa高清大片在线同性、Cur inhibits human originated CML CD34~+ cell growth中文自拍日韩在线播放视频免费、induces cell apoptosis, and enhances STI571 to down-regulate the expression of p210~, finally inhibit cell growth and induce cell apoptosis.

從以上實驗結果我們得出如下結論： 1、Cur抑制K562細胞增殖、誘導細胞凋亡的作用可能與其下調p210~日本三级网站网址大全:、蛋白酪氨酸磷酸化水平以及抑制下游p-Erk1/2永久毛片在线看、c-myc等信號分子有關； 2国产主播在线播放、Cur協(xié)同STI571抑制K562細胞增殖亚洲狼友视频网站、誘導細胞凋亡的作用可能與其下調p210~、蛋白酪氨酸磷酸化水平以及抑制Hsp90和下游PKC等信號分子有關一级a在线看片； 3人人插人人摸人人插、Cur可逆轉K562/G01細胞對STI571的耐藥性，并與STI571協(xié)同抑制K562/G01細胞增殖和誘導凋亡日本黄色片A三级三级三级，其抑制K562/G01細胞增殖尤物视频网址免费看、誘導細胞凋亡的作用可能與其下調p210~、蛋白酪氨酸磷酸化水平以及抑制下游Procaspase-3和NF-κB等信號分子有關国产区露脸在线视频； 4韩国精品视频在线一区二区、Cur可抑制來源于CML患者骨髓的CD34~+細胞的增殖并誘導其凋亡，還可協(xié)同STI571下調CML CD34~+細胞p210~表達日韩清纯无码自拍视频网站，進而協(xié)同抑制細胞增殖一级a片欧美在线视频、誘導細胞凋亡。