The history of studies on insect hemolymph inhibition cell apoptosis were discussed, and cell cultures supplementation with insect hemolymph were investigated, which suggested that there were apoptosis-inhibiting component from insect hemolymph, and they were just different protein.

通過對昆蟲血淋巴抑制細(xì)胞凋亡研究歷史的探討亚洲影视在线，發(fā)現(xiàn)昆蟲血淋巴中包含抑制細(xì)胞凋亡的組分黑人永久免费刺激视频，并且在昆蟲血淋巴中含有抑制細(xì)胞凋亡的不同分子量蛋白欧美日韩午夜福利大片在线播放，說明昆蟲血淋巴介導(dǎo)的抗細(xì)胞凋亡效果可能由不同的蛋白執(zhí)行。

Results The p53 gene mutation rate of colorectal cancer was 50%. The rate of apoptosis of tumor cells having mutation of p53 gene (22.11%) was much lower than that having no p53 mutation (40.57%, P＜0.05). Heteroploid cells had been found in 10/13 tumor samples with p53 gene mutation and 6/13 samples without p53 mutation (P＜0.05). The rate of apoptosis had no difference between the heteroploid cases and diploid cases.

結(jié)果 大腸癌標(biāo)本p53基因突變率為50%免费黄色视频A片子；其中突變組腫瘤細(xì)胞凋亡率(22.11%)明顯低于未突變組細(xì)胞凋亡率(40.57%)，(P＜0.05)国产初高中精品网；突變組異倍體細(xì)胞百分率(占76.9%)明顯高于未突變組(占46.2%)欧美一级abbb，(P＜0.05)；異倍體腫瘤細(xì)胞凋亡率為34.0%鲁在线视频，二倍體腫瘤細(xì)胞凋亡率為40.7%欧美性爱免费看视频，二者差異無顯著性(P＞0.05)。

Apoptosis of lymphocytes in bone marrow, spleen, thymus and bursa was detected by light microscope, electron microscope and terminal- deoxynucleotidyl transferase UIP nick end labeling. The results showed ALV-J could induce incoordinate apoptosis of lymphocytes in thymus, bursa and spleen, which was mainly found in early time. And the apoptosis was much more obvious in thymus than in other immune organs.

以電鏡觀察和HE染色色色色8、原位末端標(biāo)記染色光鏡觀察對ALV-J感染和混合感染雞的骨髓欧美三级电影网址、脾臟、胸腺国产精品无码AV在毛片、法氏囊等免疫器官中淋巴細(xì)胞的凋亡進(jìn)行了形態(tài)學(xué)研究国内永久第一免费福利视频，首次發(fā)現(xiàn)ALV-J可誘導(dǎo)胸腺亚洲精品愉拍偷拍网址、法氏囊和脾臟中不同程度的淋巴細(xì)胞凋亡97re精品视频在线观看，凋亡主要見于早期，ALV-J與REV混合感染時淋巴細(xì)胞凋亡尤為顯著狼友视频在线观看的，以胸腺中最為明顯www.国产无码。

Under optical microscope, the cell of NS arrayed as nest, the nuclear was big, deep-stained, thickened-karyotheca, chromatin granulated, Pathologic karyokinesis can be seen.

DDP組癌巢周圍可見少量纖維組織增生、局灶性壞死欧洲亚洲一级毛片一区二区高清视频，并可見炎細(xì)胞浸潤中文字幕网站在线观看；LNT組鏡下見少量細(xì)胞凋亡，凋亡細(xì)胞變小国产精品国产免费，變圓日韩午夜在线试看网站免费视频，核固縮，胞漿濃縮亚洲欧美日本一区，染色加深好痛好爽高潮来了视频在线观看，凋亡細(xì)胞周圍未見炎癥反應(yīng)；LNT+DDP組與LNT組鏡下大體相同超碰人人99，但可見到較多量的凋亡細(xì)胞国产免费午夜福利天堂AV。

Each group with drug had effect on inhibiting Lewis lung cancer tumor,LNT DDP had the best effect,46.2%,the rate of inhibitory effect of LNT、DDP was 35.6%欧美日韩国产成A免费网站、39.7%respectively.3.Under optical microscope,the cell of NS arrayed as nest,the nuclear was big,deep-stained,thickened-karyotheca,chromatin granulated,Pathologic karyokinesis can be seen.

DDP組癌巢周圍可見少量纖維組織增生性爱五月、局灶性壞死，并可見炎細(xì)胞浸潤免费观黄色视频；LNT組鏡下見少量細(xì)胞凋亡99热爱精品九九九九，凋亡細(xì)胞變小，變圓免费国产A片一级，核固縮av大陆老熟女性爱，胞漿濃縮日韩台精品电影在线观看，染色加深，凋亡細(xì)胞周圍未見炎癥反應(yīng)美女在线操；LNT DDP組與LNT組鏡下大體相同国产精品免费一区二区三区在线看，但可見到較多量的凋亡細(xì)胞。

He apoptosis of retinal ganglion cells could be observed one day after injury, increased by the 4th day, and reached a climax by the 7th day in the control group. Apoptosis of RGCs was detected with maniple at 4～10 days after injury in the experimental group and the rate of apoptosis in RGCs decreased significantly compared to the control group at 4～21 days.

UNEL染色及RGCs凋亡率：C組和D組在傷后第1天TUNEL染色可見凋亡細(xì)胞全黄a一级毛片，第4天明顯增多国产宅男精品，至第7天達(dá)高峰。A組和C組在傷后第4～第10天僅可見少量的凋亡細(xì)胞亚洲欧美另类在线第一页，在第4～第21天久一精品，與B組、D組比較欧美国产综合一区，RGCs凋亡率明顯減少AAAAA西欧黄片。

The researching result of this field is conflicting in these years. Some researchers consider it as apoptosis, while some necropsy results don\'t support the hypothesis. Our study is to investigate the protein expression and biochemistry changes under oxidative stress to determine if apoptosis happens.

多巴胺能神經(jīng)元的死亡是凋亡還是壞死目前的研究結(jié)果不一，有許多的研究說明在氧化應(yīng)激的條件下国产网站在线看福利午夜r，細(xì)胞的死亡是凋亡男女自拍视频网站，而部分尸檢結(jié)果不支持凋亡的學(xué)說，本實(shí)驗(yàn)擬從蛋白表達(dá)及生化學(xué)方面觀察氧化應(yīng)激后細(xì)胞有無凋亡的發(fā)生亚洲欧美日韩国国产综合。

Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

結(jié)果 (1)ATP對U937細(xì)胞的增殖有按摩明顯的阻抑作用67194熟妇人妻欧美日韩，加藥48h后增殖的抑制率可達(dá)43%以上；(2)ATP處理的U937細(xì)胞周期發(fā)生改變igao视频在线观看播放，G1期細(xì)胞數(shù)按摩明顯增多欧美一级精品影院，ATP作用24h時G1期前出現(xiàn)亞二倍體峰——凋亡峰，凋亡細(xì)胞數(shù)為3.4%欧美一级裸体视频在线观看，作用48h時凋亡細(xì)胞數(shù)增多為22.7%看中国免费黄色视频A；(3)ATP處理的U937細(xì)胞首先在核內(nèi)染色質(zhì)濃縮成半月形貼近核膜，逐漸向核膜外移動电影一区小说区偷拍区，進(jìn)入胞漿內(nèi)再移向質(zhì)膜內(nèi)外面欧美熟女高潮，緊貼質(zhì)膜外面再逐步脫離細(xì)胞體，進(jìn)入細(xì)胞基質(zhì)中欧美性爱有码，成為游離的凋亡小體在线播放免费不卡视频。

Methods Cell culture,flow cytometry,HE staining,Nigrosine staining and electron microscopy were used. Results (1)ATP could inhibit the proliferation of U937 cells with inhibition rate over 43% after 48hour ATP treatment;(2)ATP treated U937 cells numbers increased obviously in G1 phase,and apoptosis peak appeared before G1 phase,apoptotic cell number was 3.4% for 24hour,and was 22.7% for 48hour of ATP treated U937 cells;(3) Nuclear chromatin condensed into sperical masses bound cytoplasma membrane formed apoptotic bodies which is shed from membrane surface into intercellular medium;(4) Apoptotic bodies were nigrosine staining negtive.

結(jié)果 (1)ATP對U937細(xì)胞的增殖有明顯的阻抑作用，加藥48h后增殖的抑制率可達(dá)43%以上激情亚洲国产另类图片在线观看网站；(2)ATP處理的U937細(xì)胞周期發(fā)生改變国外真实呦女酱网址，G1期細(xì)胞數(shù)明顯增多，ATP作用24h時G1期前出現(xiàn)亞二倍體峰——凋亡峰欧美性爱福利，凋亡細(xì)胞數(shù)為3.4%人人操人人人，作用48h時凋亡細(xì)胞數(shù)增多為22.7%；(3)ATP處理的U937細(xì)胞首先在核內(nèi)染色質(zhì)濃縮成半月形貼近核膜变态欧美亚洲日韩女优电影，逐漸向核膜外移動AAA国产的免费视频，進(jìn)入胞漿內(nèi)再移向質(zhì)膜內(nèi)外面av付费在线网站，緊貼質(zhì)膜外面再逐步脫離細(xì)胞體，進(jìn)入細(xì)胞基質(zhì)中免费能可以看黄的网站，成為游離的凋亡小體一区二区欧美黄色。

Yam steroid saponins and sapogenin are resulting in MCF-7 cells apoptosis by arrested the G0/G1 phase, increased the p53 protein with promoting apoptosis and decreased the AKT protein with anti-apoptosis and the BRCA-1 protein with repairing DNA. And, SPG has the excellent anti-cancer effect on MCF-7 cells may be due to induced much of the p53 protein in the early treated stage.

進(jìn)一步的研究發(fā)現(xiàn)，山藥固醇類皂及皂元抑制MCF-7乳癌細(xì)胞的功效在线视频免费观看欧洲，主要是誘發(fā)G0/G1期停滯国产精品免费午夜视频，造成細(xì)胞凋亡；其透過的機(jī)制是經(jīng)由p53促凋亡蛋白的增加玖玖爱www，及AKT與BRCA-1等抗凋亡蛋白被抑制黄片WWWWWWWWWW，達(dá)到雙重細(xì)胞凋亡之抗癌效果；其中SPG處理之MCF-7肺癌細(xì)胞具早期大量誘發(fā)p53蛋白的特性凹凸视频黄色网站在线观看，或許是SPG優(yōu)異抗癌效果之原因美女被cao喷水视频在线观看。