This paper was also studied primarily on the decolor of PHA. Physical and chemical methods were all used to decolor the organic phase after the recovery by CHC13 from the cells.

另外，本論文對(duì)PHA的脫色作了初步探索美国一级牲交黑大粗硬大片学校，實(shí)驗(yàn)就物理方法和化學(xué)方法針對(duì)氯仿提取細(xì)胞后得到的PHA有機(jī)相進(jìn)行了脫色處理亚洲欧洲国产码专区在线男男。

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Macerate 1.0g of the powdered sample with 20.0 ml of chloroform plus 0.3ml of 25％ NH_4 OH in a glassstoppered flask overnight.

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The result of chemistry show-colour analysis shows ,β―sitosterol extract was soluted in chloroform , and add vitriol , the interface of double liquid layer showed rose colour .β―sitosterol extract was soluted in acetic acid and add three chloridize iron--acetic acid—vitriol solution , it showed blue , then became pansy .

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First, removing protein by Sevage method. According to literature, it had best efficiency when polysaccharide liquid 2mg·mL~(-1) was treated with chloroform: n-butanol (3:1) and repeated for 3 times. Second, moving pigment with H_2O_2. In order to shorten the time and prevent the broken of structure of polysaccharide, the experiment was operated at 37℃. The following were dialyzation, concentration(60℃) and freeze-drying.

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Methods 0mit and alter some work procedure including distillation,store reagent that show color,either the order of reagent procedure or recycle of the waste trichloromethane.

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The results showed: when TLC solvent system was: chloroform - methanol - diethyl amine = 90:9:1, alkaloids from lotus leaves were well isolated, and five components were isolated and identified by spraying Dragendorff reagent. The optimal conditions of HPLC solvent system were: methanol- water - diethyl amine = 75:25:0.0125. Under the conditions, components of alkaloids from lotus leaves were separated very well, and ideal RP-HPLC peaks were obtained.5、Methods of High Speed Counter Current Chromatography and preparative High Performance Liquid Chromatography applied to identify and purify alkaloids from lotus leaves were set up. When solvent system of HSCCC was: chloroform - methanol - water (pH=4.00)= 4:3:2, speed of chromatogram was 700rpm, flow speed of the mobile phase was 2mL/min, four pure components relatively were attained by isolation and purification.

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