GV oocytes with normal shape were not obtained in the group which was stored at 20℃ for 24 h and at 25℃ for 14 h; A blastula rates (64%) of all groups in average was gotten in culturing 2-cell in vitro, and there is no significant difference in temperature and time of storage. After transplantating some of the 2-cell embryos which were obtained from GV oocytes stored at 15'C for 8 h, normal infant mice could be obtained.

在20℃下保存24h和25℃下保存14h時(shí)www.zachiew.com，難以獲得形態(tài)正常的GV期卵一级A片试看片；體外受精獲得的2細(xì)胞期胚經(jīng)體外培養(yǎng)欧美日韩区国产区，總體上有64%的胚胎發(fā)育至擴(kuò)張囊胚一级AⅤ，未見有保存溫度和保存時(shí)間的顯著影響，且利用在15℃保存8h后的GV卵獲得2細(xì)胞期胚的移植獲得正常新生小鼠经典1页日韩中文字幕视频。

The first part consists of three experiments:(1) The rings were incubated in KH, 20, 50 mmol/L 〓 for 1 house, relaxation in response to the EDHF stimuli A23187 in 30nmol/L U46619-induced preconrtaction in the presence of 7 μ mol/L indomethacin, a cyclooxygenase inhibitor, 300μmol/L LNNA, a nitric oxide biosyhnthesis inhibitor, and 1mmol/L tetraethylammonium , a 〓 blocker, or 3 μmol/L glibenclamide , a 〓 blocker, was compared with the control;(2) After the arteries were incubated in KH, UW solution or HTK solution at 4℃ for 4 hours, endothelium-derived relaxation (percentage of 30nmol/L U46619 precontraction) was induced by A23187 in the present of 7 μmol/L indomethacir and 300μmol/L LNNA;(3) After incubation with KH, UW solution and STH (either at 37℃ in oxygenated organ chamber or at 4℃ in a refrigerator for 4 hours), endothelium-derived relaxation (percentage of 30nmol/L U46619 precontraction) was induced by A23187 in the present of 7 μ mol/L indomethacin and 300 μmol/L LNNA.

第一部分研究結(jié)果：（1）單純浸泡于KH的冠狀動(dòng)脈A23187能引發(fā)66.67％的血管舒張反應(yīng)日本少妇性爱视频免费，經(jīng)TEA及20mmol／L、50mmol／L鉀離子作用后天堂网AV中文，血管舒張反應(yīng)程度顯著降低在线视频日，但經(jīng)GBM作用后改變不明顯；（2）與保存于KH的冠狀動(dòng)脈相比无码人中文字幕黑人，A23187引發(fā)的血管舒張反應(yīng)程度国产91视频免费看，保存于UW液的明顯下降国际免费黄色网站，保存于HTK液的無明顯變化；（3）在37℃條件下00pao免费在线视频，血管環(huán)浸泡于STH出現(xiàn)緩慢輕微的舒張反應(yīng)A片毛在线是免费观看，浸泡于UW液初期出現(xiàn)短暫收縮反應(yīng)美女一级日逼毛片，但此后主要以舒張為主：在37℃條件下在线亚洲国产成人，血管經(jīng)UW液保存后，U46619引發(fā)的收縮反應(yīng)程度降低成年男人黄网站色大片免；不論在37℃或4℃條件下欧洲美女一级牲交不卡，A23187引發(fā)的血管舒張反應(yīng)，經(jīng)UW液保存后明顯下降国产精品视频一级香蕉AAA，但用STH保存后變化不明顯五月婷婷激情四射。

Monitor Memories are made of this 記憶是這樣煉成的 Sep 3rd 2009 From The Economist print edition Computing: Memory chips based on nanotubes and iron particles might be capable of storing data for a billion years 計(jì)算機(jī):毫微管和鐵粒子造存儲(chǔ)芯片,數(shù)據(jù)保存能達(dá)十億年 FEW human records survive for long, the 16,000-year-old Paleolithic cave paintings at Lascaux, France, being one exception.

人工記錄很少能長期保存。

Test teaching results of many years proved that comprehensive application,such as field collection,marcket purchase,natural cultivation,simple inoculation and other materials for preparing slides and materials collection methods as well as temporary preservation,dry preservation at room temperature,cryodrying preservation and cryopreservation,could meet the need of slide making of fungus,bacterium,virus,nemathelminth and other typical disease in a long term.

多年的實(shí)驗(yàn)教學(xué)證明愉拍自拍视频在线播放网站下载安装，綜合應(yīng)用田間采集在线视频国产欧美、市場(chǎng)購買、自然培養(yǎng)欧美bbbbbb、簡(jiǎn)易接種等制片材料采集方法與臨時(shí)保存欧美性爱首页、室溫干燥保存、低溫干燥保存免费无遮挡黄色视频、冷凍保存等保存技術(shù)人妻AV无码久久系列，可以長期滿足真菌性病害各亞門、細(xì)菌性亚洲无码第一页、病毒性及線蟲性等典型病害制片材料的需要182com三级黄片。

This essay explores using micosatellite DNAs to study the genetic diversity, and its major contents are as follows:The contrast among different methods of feces preservation: The methods are that feces was respectively stored in the field during collection at a low temperature after drying at 60 ℃, stored in 75% ethanol, stored in 100% ethanol and directly stored at a low temperature(in the attemperator added the biological ice bag),The result showed that DNA could be extracted successfully from the feces in a short storing period with any of these methods.

本文應(yīng)用微衛(wèi)星標(biāo)記來研究普氏原羚遺傳多樣性，主要研究內(nèi)容如下：一动漫精品无码、不同普氏原羚糞便保存方法比較：通過將普氏原羚的糞便在野外分別采取60℃干燥后低溫保存欧美特黄一级A片日韩一区二区三区，75％乙醇保存，100％乙醇保存国产在线精品国际自拍AV，直接低溫保存国产av天堂久久久久。

The study indicated that the effects of self-designed KYL solution on rat liver preservation are similar to that of UW solution.

結(jié)果 KYL液保存的大鼠肝臟在保存16 h以內(nèi)各時(shí)相膽汁流出量均較UW液保存者高（P＜0.01，灌注流出液AST曰韓黃色視頻、ALT和LDH含量與UW液保存者相近东北老妓女叫床对白视频，肝細(xì)胞內(nèi)鈣離子濃度較UW液保存者低（P＜0.01； KYL組保存24及48 h時(shí)国产看片地址，MDA含量低于UW組激情视频在线网，SOD含量高于UW組（P＜0.01）；光特黄特色的大片观看免费视频、電鏡觀察兩者形態(tài)學(xué)變化基本一致欧美喷水性交。

Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

結(jié)果 a經(jīng)程序冷凍液氮保存方法處理后欧美亚洲另类国产18p，髕腱的最大載荷無明顯下降，細(xì)胞活性得到了較好的保存久久精品亚洲欧美vA，組織學(xué)觀察冷凍損傷較－80℃深低溫保存方法輕微在线免费看黄片视频快点现在；b程序冷凍液氮保存處理的移植物在術(shù)后未表現(xiàn)明顯的排斥反應(yīng)，且免疫反應(yīng)隨時(shí)間的推移而下降全裸美女私拍精品视频在线观看免费；c移植后3周国产一级一级，各組移植物的最大載荷無顯著差異(P＞0.05)，移植后8周日韩精品久久一区，程序冷凍液氮保存組移植物的最大載荷(55.87±1.86)N優(yōu)于－80℃深低溫保存組(52.14±2.79)国产精品经典三级网站，而和自體移植組相近(57.70±2.76)N；d從組織學(xué)觀察看1级黄色片，－80℃深低溫保存組和程序冷凍液氮保存組移植后的愈合過程均和自體移植組相似一级毛片AAAAAA免费看，而程序冷凍液氮保存組的愈合過程和組織學(xué)行為更接近于自體移植組。

Results 1.After treated by:program freezing,the maximum load of the allografts has no evident decrease and the cell activity of allografts was preserved well,the frozen hurt after program freezing was more slight than －80℃ deepfrozen,2.No evident reject reaction can be seen during the healing process after deep frozen and the reject reaction declined as the time pass by.3.Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

結(jié)果 a經(jīng)程序冷凍液氮保存方法處理后黄页视频网址，髕腱的最大載荷無明顯下降日本aaa级黄色网，細(xì)胞活性得到了較好的保存，組織學(xué)觀察冷凍損傷較－80℃深低溫保存方法輕微欧美视频v；b程序冷凍液氮保存處理的移植物在術(shù)后未表現(xiàn)明顯的排斥反應(yīng)高清无码黄三级，且免疫反應(yīng)隨時(shí)間的推移而下降；c移植后3周欧美三级网站在线免费观看，各組移植物的最大載荷無顯著差異(P＞0.05)美女在线A级毛片，移植后8周，程序冷凍液氮保存組移植物的最大載荷(55.87±1.86)N優(yōu)于－80℃深低溫保存組(52.14±2.79)亚洲h版在线，而和自體移植組相近(57.70±2.76)N日本黄色网站进入入口；d從組織學(xué)觀察看，－80℃深低溫保存組和程序冷凍液氮保存組移植后的愈合過程均和自體移植組相似国产AV一区网站，而程序冷凍液氮保存組的愈合過程和組織學(xué)行為更接近于自體移植組午夜精品乱人伦小说区。

Cell embryos were cultured in vitro, then viability was observed. The results showed the fertilizing rate in vitro of the group for GV oocytes with cumulus cells after maturating which were conserved at 10℃ for 24 h, 15℃ for 14 h, 20℃ for 8 h and 25℃ for 4 h was 14%, 9%, 10% and 10% respectively, and with the advance of storage temperature and extension of storage time, the rate of 2-cell after maturating-fertilizating of the GV oocytes decreased significantly.

其結(jié)果，在10℃下保存24h一级做a午爰片久久毛片A片午、15℃下保存14h欧美色淫淫、20℃下保存8h和25℃下保存4h后，其體內(nèi)附有卵丘細(xì)胞的GV卵的體外成熟－體外受精后的2細(xì)胞率分別為14%精品爱99视频在线、9%美女黄网站国产免费久99、10%和10%，隨著保存溫度的提高和保存時(shí)間的延長97超级对对碰，帶有顆粒細(xì)胞GV期卵的比率明顯降低免费一级黄色片操逼播放，同時(shí)其GV期卵經(jīng)體外成熟及體外受精后的2細(xì)胞率明顯降低。

This article introduced based on the Labview8.0hypothesized sine, the cosine, the square-wave, the saw-tooth wave,the triangle wave signal generating device realization May accordingto the need, the change profile frequency and the peak-to-peak value,preserves the profile the analysis parameter to assign the document,and introduced based on the USB data acquisition card dummy signaloutput LabVIEW,虛擬儀器,保存數(shù)據(jù),信號(hào)發(fā)生器 LabVIEW, the hypothesized instrument, preserves the data, the signalgenerating device

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