Based on the conservation of miRNAs sequence, we compared the known miRNAs among five mam-mals, i.e., human, mouse, cattle, pig and dog with the sequence of sheep genome that is highly homologous to goat genome, published on the NCBI, and 11 candidate miRNAs were eventually obtained. RT-PCR analysis showed the expression of the 11 miRNAs in brain and 5 in liver, indicating that they might be novel miRNAs. The methodology provides an alternative approach to the exploration of new miRNAs in goat.

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The influence of position of the substitution, strength and number of donors and acceptors, and their coplanarity and asymmetry is discussed. The results indicate that the molecules may have larger first hyperpolarizabilities when the molecules have stronger and more donors and acceptors, large asymmetry of HOMO and LUMO, better coplanarity and higher accentricity. This work attemps at finding out the relationship between the structure and the property, thus providing theoretical basis for designing nonlinear optical molecules.

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At the normal state, porphyrin molecules do not have EPR signal. However, light excitation may cause a transition of porphyrin molecules from singlet to triplet. At the same time, the change from diamagnetism to paramagnetism occurred to porphyrin molecules. In this case, the two un-paired electrons are nearby each other and cause a strong interaction, giving EPH signal with sharp peak at g = 2.000. The g value increased with an increase in electron cloud density.

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At the normal state, porphyrin molecules do not have EPR signal. However, light excitation may cause a transition of porphyrin molecules from singlet to triplet. At the same time, the change from diamagnetism to paramagnetism occurred to porphyrin molecules. In this case, the two un-paired electrons are nearby each other and cause a strong interaction, giving EPR signal with sharp peak at g =2.000. The g value increased with an increase in electron cloud density.

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The conclusions were as follows: theνO-H andνC=O IR absorption maxima shifted towards higher wave numbers after imprinting 17β-estradiol on TFMAA-co-TRIM copolymer with the red shift of theνO-H groups being apparent after precipitation polymerization for 16h while that forνC=O groups being observed after polymerization for 24h. A strong interaction between TFMAA and 17β-estradiol was confirmed by the high selectivity for 17β-estradiol, as indicted by the values of the separation factor of isomers of 17β-estradiol/17α-estradiol (2.28) and the imprinted factor (3.01). Particle diameter of TFMAA-co-TRIM polymer was between 300 nm and 1.5μm, which suited well for solid phase sorbent throughout at low column pressure. The recognition of imprinting TFMAA-co-TRIM polymer for sterol molecules was driven by enthalpy eluted with acetronitile, and low temperature was in favor of the separation of sterol structure analogues on imprinting column. TFMAA-co-TRIM polymer possessed of determinate anti-heat stability, with melting point beginning at 255.84℃, Tp=257.40℃, control TFMAA-co-TRIM polymer and imprinting TFMAA-co-TRIM polymer were respectively decompounded at 267.79℃and 343.11℃, and solid micro-extraction noddle prepared by the special polymerization also showed definite recognition for 17β-estradiol by GC/MS detecting at 270℃. By selection of various washing and elution solvents, elution reagents of close polymerization system were of more advantage in template molecules retention and recognition on molecularly imprinted solid phase extraction column. At a certain extent, progesterone, 17α-estradiol, 17β-estradiol and 4-androstene-3, 17-dione could be intercepted on the MISPE column. Especially, MISPE had high selectivity for progesterone, and imprinting TFMAA-co-TRIM polymer could achieve adsorption balance within 50 min by absorption kinetics test for 17β-estradiol. However, MISPE column showed better selectivity and enrichment property for 17β-estradiol than C18 and CSPE columns according to the data from HPLC and GC/MS analyses. Recovery of 17β-estradiol on MISPE column was up to 85.5% while when prime extracting solution of milk powder was sampled, the recovery of CSPE and C18 columns were 43.7% and 30.7%, respectively.

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There are two independent molecules of ZnL or Co(IIL and two water molecules in one asymmetric unit of complex 1(or 2), the crystal structure of CuClL1·2H2O consists of one molecule of CuClL1 and two water solvate molecules in the asymmetric unit, while the ZnL·CH3OH (4) comprises of one molecule of ZnL and a methanol molecule in per unit.

在這四個(gè)金屬配合物中，配合物(1)和配合物(2)的每個(gè)不對(duì)稱單元中有兩個(gè)獨(dú)立的ML 分子和兩個(gè)水分子曰本Xⅹ，配合物(3)的每個(gè)不對(duì)稱單元中有一個(gè)CuClL1分子和兩個(gè)水分子激情AAAA片，而配合物(4)的每個(gè)不對(duì)稱單元中只有一個(gè)ZnL 分子和一個(gè)甲醇分子。

There are two independent molecules of ZnL or Co(IIL and two water moleculesin one asymmetric unit of complex 1(or 2), the crystal structure of CuClL_1·2H_2O consistsof one molecule of CuClL_1 and two water solvate molecules in the asymmetric unit,while the ZnL·CH3OH (4) comprises of one molecule of ZnL and a methanolmolecule in per unit.

在這四個(gè)金屬配合物中亚洲一区欧美日韩，配合物(1)和配合物(2)的每個(gè)不對(duì)稱單元中有兩個(gè)獨(dú)立的MLZn(IIL免费网站日本永久免费观看、CoL分子和兩個(gè)水分子，配合物(3)的每個(gè)不對(duì)稱單元中有一個(gè)CuClL_1分子和兩個(gè)水分子，而配合物(4)的每個(gè)不對(duì)稱單元中只有一個(gè)ZnL分子和一個(gè)甲醇分子免费看一级A片裸体舞。

In this thesis,the AM1,MNDO,MINDO/3(mainly AM1)and INDO/S-CI semiempirical MO methods were used toinvestigate the excited-state intramolecular protontransfer reactions of salicylic acid derivatives—salicylic acid,methyl salicylate,salicylaldehyde,o-hydroxyaceto-phenone,salicylamide and 3-hydroxy-picolinamide (6 conformers and 2-3 anion species);2-(2'-hydroxy-5' methylphenyl) benzotriazole(4 conformers),2-(2' hydroxyphenyl) benzimidazole (3 conformers and 3anion species),Bis-2,5-(2-benzoxazolyl)hydroquinone(3 conformers),2-(2'-hydroxyphenyl)benzothiazole(2conformers) and 7-azaindole dimer (2 conformers).Theinvestigations were described as follows.Geometry optimization,relative stability andhydrogen bonding energy First,for sylicylic acid derivative molecules,the AM1,MNDO and MINDO/3 methods were used toinvestigate ground-state geometry optimization,energies,relative stabilities and hydrogen-bondingenergies on the five kinds of the molecules(designing 6 conformers and 2-3 anion species).Comparing with experimental data,the optimizedgeometry,the order of stability,the hydrogen-bonding energies and the distances between O-O in O-H..O hydrogen bonds by AM1 method were in agreementwith the experimental data,however,the C-C bondlengths optimized by MNDO and MINDO/3 were longer,C-O and O-H bond lengths were shorter;for C-N bondlengths,the results opitimized by MNDO method werethe same as those by AM1 method,nevertheless the C-Nbond lengths given by MINDO/3 method were muchshorter.For some sylicylic acid derivatives(e.g.methyl salicylate,salicylamide),the order ofstabilities on the conformers given by MNDO andMINDO/3 methods were not in agreement with theexisting conformers deduced by experimental methods,and the hydrogen bonding energies calculated by MNDO.and MINDO/3 methods were smaller.Second,the studyon the other systems found that the optimizedgeometry of the proton-transfered product with INDOmethod could not be obtained,only could theoptimized geometry of reactant be obtained,and thecalculated hydrogen bonding energies were greater.Many results of calculation indicated that the studyon the excited-state intramolecular proton transferreaction system using AM1 method was suitable andreliable.

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Results show that intramolecular hydrogen transfer is not favored in view of the energy trend along IHT coordinate in the region such as hypericin's peri region, where two hydrogens of hydroxyl groups bond with a common oxygen of carbonyl group and exists two IHBs in the peri region. However, intramolecular proton transfer often occur in the molecule with hexahydric cycle hydrogen bond, where only one pair of adjacent hydroxyl group and carbonyl group.

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Based on the changes in the spectrum characters of guest molecules producedthrough inclusion complex formation with cyclodextrins, we have carried out two aspects of investigation:First, the mechanisms for the host-guest recognition reactions have been studied, such as the interactions between 3-CD and curcumin, the main constituent of the rhizomes of the plant Curcuma longa, the recognition of p-CD towards rubidate, the effective component of the Chinese traditional medicine madder, and the reactions of p-CD towards the antileprotic drug dapsone and the antiinflammatory drug nabumetone: The formation of supramolecular complex between P-CD and guest molecule has been discussed and verified in virtue of multitudinous means such asultraviolet-visible absorption, fluorescence emission, infrared spectrogram and so on. The influence of addition of linear alcohols on the supramolecular system has been studied and the reason why the noncovalent interactions in the three-component system could result in a decrease in the apparent association constant of the inclusion complex has been revealed.

本論文基于環(huán)糊精對(duì)被包結(jié)客體光譜性質(zhì)的改變，開展了兩大方面的研究：β-環(huán)糊精對(duì)姜黃屬植物根莖主要成分—姜黃素亚洲精品黄色中国、中藥茜草有效成分—茜草雙酯AV黄色网站大全、抗麻風(fēng)藥物氨苯砜及抗炎藥物萘丁美酮的主-客體識(shí)別反應(yīng)機(jī)制研究：借助超分子包合物的紫外吸收光譜、熒光光譜或紅外吸收光譜等手段777琪琪午夜理论电影网在线，通過多種方法探討并驗(yàn)證了超分子包合物的形成黄色av一级片在线；研究了直鏈醇的加入對(duì)超分子體系的影響，揭示了三元體系中非共價(jià)相互作用導(dǎo)致超分子包合物表觀結(jié)合常數(shù)降低的原因：對(duì)超分子包結(jié)反應(yīng)進(jìn)行了熱動(dòng)力學(xué)研究欧美熟妇与小伙子性爱，探討了各熱動(dòng)力學(xué)參數(shù)的變化趨勢(shì)并從熵焓互補(bǔ)的角度考察了超分子包結(jié)過程搞女人下面必网站；通過熒光猝滅實(shí)驗(yàn)進(jìn)一步揭示了水相中β-環(huán)糊精增敏客體熒光的本質(zhì)原因，從而為超分子化學(xué)提供新的理論和研究方法欧美色播电影。