Oogonium develops into early oocyte in the ovary, and then the oocyte leaves the ovary for the coelomic fluid in the form of single cell or cell mass followed by the rapid separation of the group of oocytes into individual ones. Oocyte enters into the nephridium after its maturation. The rupture of germinal vesicle marks the oocyte maturation. Oocyte in the coelom does not have follicle membrane and vitelline membrane is formed and developed by the oocyte itself. Smaller oocyte (0μm in diameter) is round, and larger ones (≥60μm in diameter) is ovate. The short and long diameters of a morphologically mature oocyte are about 115—120μm and 140—145μm respectively, and the vitelline membrane is 7—9μm thick.

卵原細胞在卵巢中發(fā)育至早期卵母細胞時期單個或成團脫離卵巢入體腔液中亚洲图区视频网，卵母細胞團細胞很快分離為單個細胞；卵母細胞在體腔液中發(fā)育成熟后進入腎管久久精品日本3级视频；生發(fā)泡破裂是卵母細胞成熟的標志小草亚洲无码不卡免费在线观看视；體腔中卵母細胞無濾泡膜，卵黃膜的形成與發(fā)育靠卵母細胞本身BBB在线；卵徑小于60μm的卵母細胞呈圓形99久久影院亚洲精品图片，卵徑大于60μm 的卵母細胞為卵圓形，形態(tài)上成熟的卵母細胞短徑約115—120μmwww.99碰.com、長徑約140—145μm狼友视频在线观看免费、卵黃膜厚7—9μm。

The oogenesis of P. esculenta can be divided into the following phases in morphological characteristics of oogonium and oocyte development: the proliferative phase of oogonium (0μm in diameter), the initial growth phase of oocyte (10—20μm in diameter), the grand growth phase-Ⅰof oocyte (20—60μm in diameter), the grand growth phase-II of oocyte (60μm×70μm to 120μm×145μm in size), the mature phase of oocyte, and the declining phase, showing a dynamic changes in oogenesis of P. esculenta .

以卵原細胞與卵母細胞發(fā)育的形態(tài)學特征為依據(jù)黄视频免费的，將可口革囊星蟲的卵子發(fā)生過程劃分為：卵原細胞增殖期(卵徑0μm)黄色毛片精品尿尿视频、卵母細胞小生長期(卵徑10—20μm)、卵母細胞大生長期Ⅰ(卵徑20—60μm)爱操AV、卵母細胞大生長期Ⅱ[大小為(60μm×70μm)—(120μm×145μm)]激情男女AA欧美、卵母細胞成熟期及退化期6 個階段，反映了可口革囊星蟲卵子發(fā)生過程的動態(tài)變化在线视频福利。

Hormone level in maturation medium affected cumulus expansion of COC, but cumulus expansion became less dependent on hormone level with time of culture. Porcine immatured oocytes, granulosa cells from small antral follicles and cumulus cells of immatured oocytes secreted cumulus-expansion enabling factors. Granulosa cells from 3-6mm follicles did not produce CEEF, but there was some CEEF in the follicular fluid of this sized follicles.

激素水平對卵丘擴展有影響无码国产日韩字幕视频播放器，但隨培養(yǎng)時間的延長，對激素的要求有逐步減少的趨勢亚洲国产一区在线视频；豬卵丘擴展不依賴于卵母細胞日韩AV黄色网址，卵母細胞核成熟也與卵丘擴展無關(guān)；GV期卵母細胞黄页视频免费看网站、小腔卵泡內(nèi)顆粒細胞和成熟卵的卵丘細胞都有較高的分泌CEEF能力一级毛片免费视频色欲；3-6mm卵泡的壁顆粒細胞不能分泌CEEF，但3—6mm卵泡的卵泡液中有CEEF色妞一区二区三区视频在线。

Hormone level in maturation medium affected cumulus expansion of COC,but cumulusexpansion became less dependent on hormone level with time of culture.Porcine immaturedoocytes,granulosa cells from small antral follicles and cumulus cells of immatured oocytessecreted cumulus-expansion enabling factors.Granulosa cells from 3-6mm follicles did notproduce CEEF,but there was some CEEF in the follicular fluid of this sized follicles.Sun xingshen, Animal Histology and Embryology

激素水平對卵丘擴展有影響亚洲人人一区二区三区，但隨培養(yǎng)時間的延長，對激素的要求有逐步減少的趨勢啪啪视频一区；豬卵丘擴展不依賴于卵母細胞2828一区二区欧美，卵母細胞核成熟也與卵丘擴展無關(guān)；GV期卵母細胞www.毛片、小腔卵泡內(nèi)顆粒細胞和成熟卵的卵丘細胞都有較高的分泌CEEF能力美日韩一区二区毛片；3-6mm卵泡的壁顆粒細胞不能分泌CEEF，但3-6mm卵泡的卵泡液中有CEEF。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分為4個部分九九久久精品无码免费一区，第一部分對影響豬卵母細胞體外成熟和孤雌激活后胚胎分裂的幾個重要條件進行了比較研究国产原创精品在线观看，確立了一種較好的培養(yǎng)方法：與顆粒細胞共培養(yǎng)，找到了一種適合豬卵母細胞體外成熟的培養(yǎng)基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸一级片免费播放，成熟率和分裂率分別為(86.7±3.35)%和(86.3±4.16)%欧美色图图区，國外報道的最高成熟率為(85.7±4.1)%；第二部分對豬卵母細胞孤雌激活的化學方法進行了研究A无码三级片电影免费，確立了化學激活豬卵母細胞的兩種最佳方法：1將成熟的去卵丘顆粒細胞的MII期卵母細胞用20μmol/Lionomycin作用30min国产精品处女一级黄片B，再將卵母細胞培養(yǎng)于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培養(yǎng)液中，卵裂率和桑囊胚發(fā)育率達到(76.7±7.6)%和(37.1±6.4)%2將成熟的去卵丘顆粒細胞的MII期卵母細胞在200μM/L的Thimerosal中處理20min超碰在线伊人网，再與8mM的DTT共孵育30min日本高清黄页，卵裂率和桑/囊胚形成率為(81.0±2.8)%和(39.6±2.7)%；第三部分對孤雌激活胚胎的培養(yǎng)條件進行了研究人人超碰人人超碰人人，確立了一種最佳的胚胎培養(yǎng)條件：在SOF簡單培養(yǎng)基中添加顆粒細胞進行前3天的培養(yǎng)一区二区视频免费，然后轉(zhuǎn)入添加胎牛血清的NCSU—23培養(yǎng)基并和輸卵管上皮細胞進行后期的培養(yǎng)，其桑椹胚和囊胚的發(fā)育率為(59.5±3.2)%黄页网站免费观看的视频高清；第四部分研究了IGF-I

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h轉(zhuǎn)為正常培養(yǎng)24h日鞋欧美综合区视频第一页，不影響卵母細胞的成熟和孤雌激活能力，并且CHX抑制后再成熟的卵母細胞經(jīng)孤雌激活發(fā)育到囊胚的比例與對照組卵母細胞相似啊啊啊作爱免费视频暴。8黄色大片一级片、體外受精后，CHX抑制后再成熟卵母細胞的多精受精現(xiàn)象顯著增加日韩一区美女在线观看，發(fā)育到桑椹胚/囊胚的比例顯著低于對照組农村老熟妇乱子伧视频。9、CHX抑制過程中皮質(zhì)顆粒仍能發(fā)生遷移免费乱伦av，但是CHX抑制會對皮質(zhì)顆粒的遷移造成不可恢復的損傷四川一级毛片美女，使再成熟的卵母細胞內(nèi)皮質(zhì)顆粒不能完全遷移。10久草亚洲视频、CHX抑制對山羊卵母細胞α-微管和微絲都沒有影響综合婷婷色色，無論是抑制后處于生發(fā)泡期的卵母細胞，還是抑制后再成熟的卵母細胞免费在线综合，微管和微絲的分布都與對照卵母細胞相似国产一级a爱免费视频在线。

objective we start with clinical research to explore the ability and mechanism of ion conduction with chinese herbs to curing ovulatory obstacle.methods adopting the methods of clinical observation random contrast.select the cases of ovulatory obstacle dividing randomly into two groups:therapeutic group and control group,the patients of therapeutic group using ion conduction with chinese herbs with act'on nourishing blood and promoting blood circulation to dissipate blood stasis when follicle grow up to 1.4cm.we take the decoction of jia jian tao hong si wu tang conducting the unilateral lower abdomen which has the dominant follicle,treating continuously for 4～8 days and observing ovulation.the patients of control group inject hcg 10000unit when follicle grow up to 1.8cm and observing ovulation.appraising two groups ovulated rate and pregnancy rate,and detect fsh、lh日韩高清成人免费播放视频网站大全、e2国产特黄精品视频、p at sixteen to eighteen days of menstrual cycle.results ion conduction with chinese herbs can significantly inspires ovulation and its effection is evidently higher than control group and has a higher pregnancy rate,no side effect of ovarian hyperstimulation syndrome.conclusion ion conduction with chinese herbs has significant function on inspiring ovulation.the target effection of treating ovulatory obstacle is exact.this method has a ovulated rate and higher pregnancy rate.

采用臨床觀察、隨機對照的方法亚洲三级网站，選擇排卵障礙的患者，隨機分為治療組和對照組，治療組在b超監(jiān)測卵泡生長達到1.6cm時男人天堂网址网站，給予具有養(yǎng)血亚洲久怡红院、活血化瘀作用的加減桃紅四物湯水煎劑，用離子導入儀將中藥水煎劑導入有優(yōu)勢卵泡的一側(cè)少腹部中文字幕日韩欧美在线，連續(xù)治療4～8時天五月天丁香六月激情综合在线，同時連續(xù)監(jiān)測卵泡排出情況。對照組在b超監(jiān)測卵泡達到1.8cm時亚洲欧美日韩久久综合，開始采用hcg10000u肌注人妻无码伊人，連續(xù)b超監(jiān)測卵泡排出情況。評價兩組排卵率及受孕率欧美综合亚洲日韩精品别内，同時在月經(jīng)周期的第16～22天抽血查fshAAA免费黄色视频、lh、e2欧美一区1080P、p一级做a爰片久久毛片首页。結(jié)果運用離子導入的方法將具有促排卵作用的加減桃紅四物湯水煎劑導入有優(yōu)勢卵泡的一側(cè)附件，其促排卵的作用優(yōu)于用hcg肌注促排卵日韩无码黄片，而且受孕率高亚洲中文字幕久久精品无码A，無卵巢過激綜合征之虞。結(jié)論中藥離子導入具有顯著的促排卵作用色色91，其治療排卵障礙的靶向作用確切一级做a爰片久久毛片A片漏，排卵率及受孕率高。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我們在取得兩種不同品系小鼠的卵丘卵母細胞復合體后欧美在线a片播放97视频，先將卵丘卵母細胞復合體置于含有多種生長因子和激素的MEM培養(yǎng)液中培養(yǎng)6小時天堂网黄色网站在线观看，此時卵母細胞已進入第一次減數(shù)分裂的前中期，并且在DIC倒置顯微鏡下可以看到濃縮的染色體激情毛片免费看，用Polscope可以發(fā)現(xiàn)明顯的紡錘體先锋影音一区二区，借助這種顯微鏡通過顯微操作將兩種不同品系小鼠來源的卵母細胞的MI紡錘體進行互換，經(jīng)過三次直流電脈沖作用后久久公开免费视频观szs，分別有91.6％的胞質(zhì)—MI核質(zhì)體對融合欧美老妇老熟妇性性性，經(jīng)過進一步的培養(yǎng)后，超過80％的重組卵母細胞排出第一極體亚洲一级精品A片免费视频，體外受精后分別有79％和77％的重組卵形成雙原核正在播放国产一区二区中文，受精后的胚胎在KSOM胚胎培養(yǎng)液中體外培養(yǎng)4天后，超過60％的胚胎發(fā)育至囊胚一级A片免费天天噜。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

結(jié)果顯示：(1)在成熟液中添加蛋白質(zhì)可以加強卵丘細胞的擴散色色五月黄片免费看，但豬卵母細胞的核成熟與其周圍的卵丘細胞擴展沒有必然的聯(lián)系，卵丘細胞擴散或成放射狀不宜作為豬卵母細胞核成熟的標準欧美亚洲日韩国产精品第6页，只有排出第一極體才能作為豬卵母細胞核成熟的標志日韩精品短片影院在线播放视频免费；(2)在豬COCs的46-48小時成熟培養(yǎng)的后23-24小時階段去除成熟液中的激素不但可以保證卵母細胞的核成熟率可以看被操的网站，而且可加強卵丘細胞的擴散；(3)在現(xiàn)有實驗條件下你懂得手机在线播放，在mTCM和mNCSU中添加10％pFF與在mNCSU中添加10％FCS相比可獲得較高的豬卵母細胞核成熟率黄片视频A级毛片在线观看；(4)在不同的成熟液中添加不同的蛋白質(zhì)對豬卵母細胞核成熟率的影響效果不一樣，但對體外受精后的早期胚胎發(fā)育影響不明顯在线儿播放你懂的；(5)成熟液種類對豬卵母細胞的卵丘細胞擴散和體外受精后的早期胚胎發(fā)育無顯著影響无遮挡一级毛片视频网站。

The cumulus cells of OCC were cut off and dispersed by 1 mL syringe. The cumulus cells were co-cultured with the immature oocytes retrieved from the COH cycles after they adherent to the bottom of the dish. The immature oocytes were experienced IVM procedures in different culture media. They were divided into 3 groups(the oocytes at germinal vesicle stage from one woman were allotted to the same group randomly). Group 1: basic culture medium+ human follicular fluid; Group 2: solution A+ cumulus cells; Group 3: solution A+ OCC+ follicle stimulating hormone + epidermal growth factor. Then, the maturation rate, fertilization rate and formation rate of available embryo were observed.

在控制性促排卵周期有未成熟卵母細胞時，將同周期成熟卵丘復合體切出部分卵丘細胞特级黄色毛毛片aaaa，用1 mL注射器抽打分散細胞图片区偷拍区视频区，貼壁培養(yǎng)。113個治療周期中三级免费在线视频，298枚生發(fā)泡期卵母細胞經(jīng)3種不同培養(yǎng)液體外成熟培養(yǎng)(同一病人的生發(fā)泡期卵被隨機分到某同一組中)：第1組28個周期中73枚：基礎(chǔ)培養(yǎng)液+卵泡液亚洲国产日韩精品怡红院；第2組40個周期中115枚：A液+分散貼壁的卵丘細胞；第3組45個周期中110枚：A液+分散貼壁的卵丘細胞+促卵泡生成激素+表皮生長因子91av视频在线观看网站。