Japonicus and analyzed the role of animal process of oocyte during ovulation and oocyte maturation. What's more, we studied the role of microtubules and microtubule organizing center upon GV migration. The oocyte of A. japonicus connects to the follicle via animal pole process. When placed in normal sea water, ovulation started spontaneously.

利用DTT誘導(dǎo)刺參卵母細胞成熟人人操人人色人人干人人舔，綜合運用光鏡又粗又大又色视频网站、電子顯微鏡及激光共聚焦技術(shù)觀察卵母細胞成熟過程人人摸人人操操日日，分析了卵母細胞表面動物極突起在卵母細胞脫濾泡中的作用亚洲午夜福利电影，以及卵母細胞動物極突起和微管組織在生發(fā)泡移動過程中所起的作用黄色操逼片。

The results showed that 1 there were no significant differences in the rates of cytoplast protrusion and enucleation between oocytes that were incubated in colchicine (0.4 μg/mL) for 0.5 h and oocytes that were incubated in colchicine (0.4 μg/mL) for 1 h, and the rate of cytoplast protusion can be 85.4% while the rate of cytoplast enucleation is 100%. 2 There was no significant difference in oocyte enucleation between oocytes treated with medium containing 0.2 μg/mL colchicine for 0.5 h and oocytes treated with medium containing 0.4 μg/mL colchicine for 0.5 h. 3 A maturation time of 18–23 h did not affect the rates of cytoplast protusion and enucleation by chemically assisted enucleation, whereas the rate of enucleation of oocytes by blind enucleation was found to decrease with a prolonged incubation time. 4 The development rates of reconstructed embryos could not be influenced by these two enucleation methods, increased from oocytes matured for 21–23 h.

結(jié)果表明： 1 卵母細胞在0.4 mg/mL的秋水仙素溶液中分別孵育0.5 h和1 h久久久精彩视频，胞質(zhì)突起率和去核率沒有顯著的差異，突起率可高達85.4%欧美成人aⅴ在线播放，去核率達到100%色呦呦一站二站； 2 0.2 mg/mL或0.4 mg/mL秋水仙素溶液將卵母細胞處理0.5 h，對去核效果沒有顯著影響色中色污网站； 3 對于體外成熟18~23 h的卵母細胞欧美性爱干，隨著成熟時間的延長，盲吸法的去核率降低不卡毛片日了日操了操，但沒有影響秋水仙素誘導(dǎo)胞質(zhì)突起的比率和去核率超碰在线综合网； 4 兩種去核方法對重構(gòu)胚的發(fā)育沒有產(chǎn)生顯著影響，但成熟21~23 h卵母細胞重構(gòu)胚囊胚的發(fā)育率顯著高于成熟18~20 h卵母細胞重構(gòu)胚囊胚的發(fā)育率www.taose。

After being dispersed by simply beat upon with syringe and adherent culture, the mature cumulus cells from mature OCCs in COH cycles, together with growth factors in the follicular fluid or extraneously supplemented, could promote the IVM of immature oocyte.

來自控制性促排卵周期的成熟卵丘細胞經(jīng)簡易吹打分散后貼壁培養(yǎng)国产一级AA片在线视频，可能能協(xié)同卵泡液中或外加的生長因子，促進未成熟卵母細胞的體外成熟美女高潮潮喷流白浆视频在线观看，而本研究技術(shù)簡易有效人人摸草碰日日，可用于挽救促排卵周期的未成熟卵。

Following the puncture of zona pellucida with two holes by injection pipette that contained donor nuclei or cells, the injection pipette was pulled back to the perivitelline space while the negative pressure was increased in the holding pipette until the polar body and karyoplasm were wiped off completely. Then a reconstructed embryo was completed by the direct injection of the donor nucleus or cell without pulling out the injection pipette.

首先以預(yù)先吸有細胞核或細胞的注射針在固定于持卵針上的卵母細胞透明帶上穿刺兩個孔18分钟处破痛哭视频在线，然后一邊緩慢地將注射針回拔至卵周隙中日批黄色一级网站，一邊逐漸增加持卵針中的負壓禁止18岁看的免费黄视频网站，直至極體與目標(biāo)核質(zhì)被完整吸入持卵針中而完成去核，最后在不拔出注射針的情況下直接注射細胞核或完整細胞進而完成重構(gòu)胚的構(gòu)建真正能看的亚洲黄色网站。

Under light microscope, the egg is an oblated and agglutinated demersal with a narrow perivitelline space; eggs ranged in diameter from 1.95 to 2.38 mm with a mean of 2.18 ± 0.03 mm; their membrane are smooth and they have 5 to 7 cannular keratose egg-filaments; the oil globules are multiple, ranged in diameter from 0.05 to 0.50 mm and in number from 7 to 145 with a mean of 28±2, the position of them migrate during embryonic development; the yolks are ivory-white with no segments, ranged in diameter from 1.00 to 2.30 mm with a mean of 1.90 ± 0.03 mm; the development phases of the embryo are variable. So they were identified as the eggs of one Beloniformes.

在光學(xué)顯微鏡下：卵呈微扁圓形98k免费网站毛片，卵徑1.95~2.38mm，粘著沉性国产在线视频区；卵黃間隙較窄野外农村妇女一级A片视频，卵黃囊呈乳白色，無龜裂黄色网欧美；卵膜平滑天天摸夜夜添添到高潮水汪汪，在其表面有5~7根細長角質(zhì)管狀卵膜絲；多油球數(shù)不定（4~145）日本va欧美大胆视频，油球徑0.05~0.50mm久久99精品久久久久久66，油球在卵黃囊中的位置隨不同的發(fā)育時期而不斷發(fā)生變化；胚體處在不同的發(fā)育階段A片一区二区三区视频大全，其特征符合頜針魚目魚卵的特征久久久久久久精品九精品。

The results showed that mercury chloride (0.5 and 1.5mg/kg BW) had obviously toxicity to the liver and the kidney of mouse, and the dose of 1.5mg/kg BW could damage the ovary function and reduce the number of superovulation oocytes.The results also showed that mercury chloride could inhibit the extruding of the first polar body and affect the quality and the viability of mouse oocyte and reduce the rate of IVF,but little impact on germinal vesicle breakdown of mouse oocyte in vivo.

結(jié)果表明，0.5和1.5mg/kg BW汞對小鼠的肝臟美女视频黄a免费久久、腎臟有明顯的毒性作用欧美一区观看，1.5mg/kg BW組還對卵巢有明顯的毒性作用，并能顯著降低超排卵的卵母細胞數(shù)淫荡视频网页；汞對體內(nèi)卵母細胞生發(fā)泡破裂沒有影響亚洲熟女偷窥综合网，但可以抑制卵母細胞第一極體的釋放，影響卵母細胞的存活率并降低體外受精率A级黄片在线视频免费。

KM strain mice immunosuppressed with 7. 5～10mg/L DEX in drinking water had the higher oocysts output and fewer dead than other groups.(4) There was no significant difference infection rate between groups inoculated with different number CSO and groups inoculated CSO with conserved different time. Trial were divided into control, groups of 50mg/kg /d, 100 mg/kg /d and 200 mg/kg /d dose of the drug to study the anticryptosporidial activities of nitazoxanide in an immunosuppressed mice model. The experimental results showed the treatment with 200mg/kg bw /day was significant inhibition of oocyst excretion. To detect the two cryptosporidiums strain C.

實驗結(jié)果表明：C.parvum蟲種能夠感染上本實驗所采用的BALB／c和昆明小白鼠兩種小鼠人人肏屄人人操，而C.andersoni沒有能夠感染本次實驗小鼠；且從小鼠感染后的排卵囊量和小鼠的生存狀態(tài)來看色88欧美日韩国产无线码，采用5～6周齡的KM鼠能獲得最大量的隱孢子蟲卵囊欧美日韩亚洲一级在线视频，小鼠的死亡數(shù)最少；每升飲水中地塞米松量為7.5mg～10mg組比12.5mg以上組小鼠死亡數(shù)量少亚洲欧洲A片，比5mg／L以下組排卵囊數(shù)量多欧美一级日本999；接種不同卵囊數(shù)和卵囊的保存時間這兩個參數(shù)均對小鼠受感染后的排卵囊情況影響不大。

These observations show that reprogramming is easier in interspecific embryos reconstructed with ES cells than that reconstructed with somatic cells, and that ES cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. Oocytes were reconstructed with outbreeding Kunming albino mouse ES cells and enucleated rabbit oocytes, and the effects of the passages of ES cells and 6-DMAP on the development of interspecific reconstructed oocytes were analyzed. The interspecific reconstructed ES-rabbit oocytes were activated either by combined two set electric pulses and 6-DMAP or by two set electr

以上結(jié)果顯示综合色久一本毛片，6－DMAP能增加胚胎干細胞異種重構(gòu)卵的卵裂率A级美女网站，對重構(gòu)卵囊胚的發(fā)育率影響不大；高培養(yǎng)代數(shù)和低培養(yǎng)代數(shù)的胚胎干細胞用于異種核移植不影響異種重構(gòu)卵的卵裂率和囊胚發(fā)育率www、/789黄片；用胚胎干細胞作為供核細胞欧美人人爱；比體細胞作為供核細胞所構(gòu)建的異種重構(gòu)胚更容易進行重編程，并且胚胎于細胞指導(dǎo)異種克隆胚胎正常發(fā)育的能力強于體細胞超级碰人人操。

The new GV preparation method we developed allowed the best visualization of chromatin distribution after Hoechst or CMA3 staining. Our results showed that GV chromatin configurations are different among different species, and thus, while chromatin condensed into a ring around the nucleolus in porcine and bovine oocytes, no such a ring was observed in caprine oocytes; Porcine oocytes were synchronized at GV1, goat oocytes at GV3n and bovine oocytes at GVf configurations before GVBD occurred, indicating that these configurations are progressive towards final maturation rather than atresia; Changes of chromatin configurations were associated with gene activity of transcription; GV chromatin configurations were affected by the environmental changes such as serum starvation and elevated temperature.

應(yīng)用該技術(shù)對豬ccc欧美在线视频观看、山羊和牛不同大小www.huangse.con、不同健康程度卵泡及體內(nèi)和體外成熟過程中卵母細胞染色質(zhì)構(gòu)型的變化發(fā)現(xiàn)：1）不同動物卵母細胞染色質(zhì)構(gòu)型不同；豬和牛卵母細胞染色質(zhì)圍繞核仁凝集形成環(huán)日日射日日干，但山羊卵則沒有此環(huán)看一级二级黄色网站。2）卵母細胞在GVBD之前都同步在某一染色質(zhì)構(gòu)型；豬同步在GV1人人操av在线，山羊GV3n啪啪啪啪啪啪啪教学视频高清无码，牛GVf，說明這些染色質(zhì)構(gòu)型代表著進行性變化激情免费视频观看网站在线观看。3）染色質(zhì)構(gòu)型的變化反映基因轉(zhuǎn)錄活動的變化国产特黄大片美女精品。4）染色質(zhì)構(gòu)型受環(huán)境變化的影響。

Japonicus.Nerve extraction substance of sea star and dithiothreitol has been reported to be effectively to induce oocyte maturation in sea cucumbers. In this study we tested their effects on oocyte maturation induction in sea cucumber A. japonicus. The results are as follows, oocyte of A. japonicus does not get mature spontaneously. Neither NES alone nor NES with follicle suspension can induce oocyte maturation. While DTT can prominently increase the percentages of germinal vesicle break down. When the concentration of DTT is between10-1 mol/L and 10-3 mol/L, the effect of maturation induction is more sufficient. When the concentration is about 10-2 mol/L, the percentage of GVBD can reach up to more than 90%.

分別利用在海參中有誘導(dǎo)作用的海星神經(jīng)提取液和二硫蘇糖醇對從成熟卵巢解剖出的刺參卵母細胞進行誘導(dǎo)成熟試驗国产下药迷奸，結(jié)果顯示黄黄网站在线观看，刺參的卵母細胞在海水中不會發(fā)生自發(fā)成熟成年黄页网站视频全免费，海星神經(jīng)提取液以及神經(jīng)提取液加濾泡懸浮液對卵母細胞沒有誘導(dǎo)成熟的作用，而二硫蘇糖醇處理可以誘導(dǎo)卵母細胞生發(fā)泡破裂日本一一video，當(dāng)DTT溶液的濃度處于10-1 mol/L到10-3 mol/L之間時欧美日韩专区，對刺參卵母細胞的促熟作用較為明顯，在10-2 mol/L左右時日韩一级视频欧美一级，卵母細胞的誘導(dǎo)成熟率可以達到90%以上。