One of two sperm get into an archegonium and fused with egg. The nucleus of fertilized egg began to divide, and the walls were formed after the third division. The eggs without fertilization divided two times and then disappeared.

雄配子體產(chǎn)生的兩個精子中的一個進(jìn)入頸卵器與卵細(xì)胞結(jié)合形成受精卵国产www黄，受精卵核到第3次分裂形成細(xì)胞壁人人摸一人人操，沒有受精的卵細(xì)胞還要進(jìn)行兩次有絲分裂后才消失。

And the research materials were often meiotic pachytene chromosomes of sex gland and mitotic prometaphase of early embryos and diapause eggs\' somatic cells.

早期黄色在线视频免费观看的网站，家蠶染色體識別和基因定位研究主要利用性腺減數(shù)分裂粗線期的染色體https://cao127.com、早期胚胎和滯育卵有絲分裂早中期的染色體作為材料，按照相對長度和部分限性易位系統(tǒng)或缺失等來進(jìn)行核型分析日本免费高清视频www。

It takes about 0.5 h for a pollen tube from germination to arrive at the micropyle. About 0.5 h after pollination, the pollen tube releases two sperms into one synergid. At 0.5-2.5 h after pollination, the egg cell fuses with one sperm to form a zygote. About 10.0 h after pollination, the division of the zygote begins. The duration of the zygote lasts about 2.5-10.0 h. However, one sperm nucleus fuses with two polar nuclei at 1.0-3.0 h after pollination, and 5.0 h after pollination the primary endosperm nucleus begins to divide.

水稻受精過程經(jīng)歷的時間表如下：授粉后国产精品久久视频，花粉在柱頭萌發(fā)；花粉萌發(fā)至花粉管進(jìn)入珠孔大約需要0.5小時狂插美女女阴道动态；授粉后0.5小時左右97性美女爽爽视频5，花粉管進(jìn)入一個助細(xì)胞，釋放精子黄片爆草中文字幕；授粉后0.5-2.5小時在线观看A片免费视频，精卵融合形成合子；授粉后約10.0小時www.BLA片，合子第1次分裂免费视频AA在线观看，合子期為授粉后2.5-10.0小時；授粉后1.0-3.0小時曰韩一级A片特黄无码，精核與兩極核融合国产欧美日韩综合精品区；授粉后約5.0小時，初生胚乳核分裂午夜视频在线观看91。

The cleavation rates and blastocyst development rates of cloned embryos were used to assess the efficiency of different operational procedure. Finally, the best combination of operational procedure, that the spindle-viewer system was used for oocytes enucleating, and donor cell was electrofused into ooplasm by electrical pulse (1.9 kV/cm, 10 ms, two) to reconstruct bovine cloned embryos.

以核移植胚胎的卵裂率国产精品15、囊胚發(fā)育率作為檢測指標(biāo)，對不同的方法所獲得的克隆胚胎的卵分裂率與囊胚發(fā)育率進(jìn)行比較黄色视频男的操女的网站，最后篩選獲得1個優(yōu)化的牛體細(xì)胞核移植操作程序国产一级毛片久久久久久，即采用Spindle view系統(tǒng)對牛卵母細(xì)胞進(jìn)行去核操作，將供核體細(xì)胞注射到卵周隙十八禁视频在线，然后通過電融合法將供體核引入去核卵細(xì)胞質(zhì)(電融合參數(shù)為1.9 kV/cm亚洲色图拍自拍，脈沖時程10 ms，方波2次間隔2 s)老师嗯啊好深再用力一点免费视频。

In conclusion, these results suggested that (1) the ageing-associated decline in fertility of female KM mouse was due to a decrease in both quantity and quality of oocytes;(2) the ooplasm from young mice did not correct the meiotic errors of aged mice, and the ooplasm from aged mice did not induce abnormal segregation of meiotic chromosome of young mice, indicating that meiotic anomalies found in the oocytes of aged mice might be related to nucleus or chromosomes and relevant factors;(3) a critical nucleocytoplasmic ratio was essential for normal maturation and segregation of meiotic chromosomes of oocytes and development to 2-cell embryos.

本研究結(jié)果表明：（1）昆明白小鼠與衰老相關(guān)的生育力下降是其卵母細(xì)胞數(shù)量減少和質(zhì)量下降的綜合結(jié)果一级a在线爱免费观看欧美；（2）年輕小鼠卵母細(xì)胞細(xì)胞質(zhì)不能糾正老齡小鼠GV的減數(shù)分裂錯誤，老齡小鼠卵母細(xì)胞細(xì)胞質(zhì)也不誘導(dǎo)年輕小鼠的GV發(fā)生減數(shù)分裂錯誤九九热,热久久,这里有精品，老齡小鼠卵母細(xì)胞減數(shù)分裂異常很可能與細(xì)胞核或染色體及其相關(guān)因素有關(guān)成人无码秒播在线观看视频免费下载；（3）關(guān)鍵的核質(zhì)比對卵母細(xì)胞正常的成熟、減數(shù)分裂染色體分離及2-細(xì)胞期胚的發(fā)育是絕對必需的快射视频在线播放网站。

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三欧美全黄视频、EGF和TGFα對卵母細(xì)胞成熟的影響未成熟卵母細(xì)胞分別在TCM199基礎(chǔ)培養(yǎng)液+血清亚洲tv精品一区二区三区、TCM199基礎(chǔ)培養(yǎng)液+BSA+EGF、TCM199基礎(chǔ)培養(yǎng)液+BSA+TGFα和TCM199基礎(chǔ)培養(yǎng)液+BSA四組成熟培養(yǎng)系統(tǒng)中成熟培養(yǎng)久久国产精品特及毛片，22小時后上述各組卵母細(xì)胞的核成熟率分別為75.2％厕拍网站、73.1％、69.8％美女曰逼黄色网站、63.5％55亚洲精品，處于第一次減數(shù)分裂末期的比率分別為16.3％、15.9％摸美黄色网站视频摸黄色网站视频摸黄、16.9％性爱网站流畅不卡、27.0％，在添加血清日韩欧美一级片视屏、EGF亚洲免费视频在线视频、TGFα各組中核成熟率顯著高于其它各組(P＜0.05)，處于末期的比例明顯低于其它各組(P＜0.05)：添加血清国产黄色视频A片、EGF中文字幕免费在线播放、TGFα各組在a-Tubulin蛋白的分布與表達(dá)上(66.6％、66.6％美国高清无码免费看bb、73.6％)明顯高于BSA組的正常率43.3％(P＜0.05)久久久久精品免视频94久久精品视频；各組CG發(fā)生遷移較好的卵母細(xì)胞的比率分別為58.8％、33.9％黄色视频bb大、54.7％国产调教免费视频免费专区、47.9％，血清組和添加EGF組CG遷移至皮質(zhì)區(qū)的比例明顯高于僅添加BSA處理組(P＜0.05)国产午夜三级片免费观看。

Most of the a-sexual embryo sacs also parthenogenetically developed into asexual seeds,which is the same as that of their maternal parent.

此外日韩激情无码一级毛片久久，平邑甜茶也形成次生極核，并在卵分裂前亚洲国产aⅴ精品一区不卡，形成胚乳游離核午夜理伦电影在线不卡717；而卵的發(fā)育，無論受精與否都要經(jīng)過3-5天休眠后分裂性爱A片，并逐漸形成小球形胚中文字幕无码永久无线无码、子葉胚。

The fertilization was processed by sperm in mature male gametophyte combining with egg cell on the top of archegonia on June 15 or so.

摘要樟子松發(fā)育成熟的雄配子體中的精子6月15日左右在頸卵器中上部與卵細(xì)胞結(jié)合av在线你懂得，進(jìn)行受精作用5060一级欧美午夜，其后，受精卵進(jìn)行游離核分裂亚洲国产欧美中文丝袜在线，形成8個子核時，開始形成細(xì)胞壁黄色网站啪啪。

In the present study, we collected cumulus cells oocyte complex from ovaries of two different strain mice. The cumulusenclosed oocytes were cultured for 6 h in MEM supplemented with growth factor and FSH. The meiotic maturation of these oocytes has progressed to pro-metaphse Ⅰ stage and the condensed chromosomes are visible under DIC microscope, metaphase Ⅰ spindle even can be detected under Polscope. The metaphase Ⅰ spindles of oocytes were exchanged under such microscopes. After electric stimuli, 91. 6% and 91. 6% karyoplasts-cytoplasm pairs were fused respectively. The resulting oocytes were cultured further in MEM and over 80% of oocytes released the first polar body. 79% and 77% of oocytes formed two pronuclei after in vitro fertilization and the embryos were cultured in KSOM supplemented with amino acids. Over 60% of embryos developed to blastocyst stage.

在本研究中我們在取得兩種不同品系小鼠的卵丘卵母細(xì)胞復(fù)合體后人妻系列影视无码专区首页，先將卵丘卵母細(xì)胞復(fù)合體置于含有多種生長因子和激素的MEM培養(yǎng)液中培養(yǎng)6小時，此時卵母細(xì)胞已進(jìn)入第一次減數(shù)分裂的前中期，并且在DIC倒置顯微鏡下可以看到濃縮的染色體三级三级三级A级全黄公司的，用Polscope可以發(fā)現(xiàn)明顯的紡錘體人妻无码天天拍夜夜爽，借助這種顯微鏡通過顯微操作將兩種不同品系小鼠來源的卵母細(xì)胞的MI紡錘體進(jìn)行互換，經(jīng)過三次直流電脈沖作用后欧美日韩精品，分別有91.6％的胞質(zhì)—MI核質(zhì)體對融合免免费看成人片永久视频，經(jīng)過進(jìn)一步的培養(yǎng)后，超過80％的重組卵母細(xì)胞排出第一極體色哟哟一区二区三区视频，體外受精后分別有79％和77％的重組卵形成雙原核国产在线国偷精品产拍，受精后的胚胎在KSOM胚胎培養(yǎng)液中體外培養(yǎng)4天后，超過60％的胚胎發(fā)育至囊胚97碰碰色。

Most sperm nuclei eventually developed into the male pronuclei, just like the normal sperms, except sperm nuclei in a few zygotes kept dense throughout the fertilization process. Dense chromosome body was seen at the nuclear area during the prophase of first mitosis, and in the middle of spindle at metaphase of first mitosis.

精子入卵后形成精核黄网站啊啊啊在线免费，大多能夠逐漸解凝、液化并膨大在线黄网视频，最終形成形態(tài)正常的雄性原核找小姐放黄片手机播放播放，但在第一次卵裂早期退化成濃縮的染色質(zhì)小體，并在胞質(zhì)分裂時隨機地分配到其中一個子細(xì)胞里的分裂溝附近2019年黄片视频。