Cleavage velocity and fragment amount of embryo were related to chromosome 21 aneuploidy.

胚胎卵裂的速度和胚胎碎片的多少與胚胎內(nèi)部染色體的非整倍性存在著相關性AV无码国产精品一区。

The snail was found to have an indirect developmental type in the early development. The fertilized eggs were oval-shaped and discoidal cleavage. Embryonic development stages were divided into cleavage, blastula, gastrulae, trochophore, and intra-membrane veliger. Larval development stages included veliger, late veliger and crawling larvae metamorphosed to juvenile.

結果表明：黃口荔枝螺卵囊高度平均為7.33 mm1024手机基地看人妻欧美，卵粒數(shù)量平均為165粒欧美爱爱一区二区视频；黃口荔枝螺的早期發(fā)育屬間接發(fā)生型亚洲国产一区二区三区青草影视，受精卵呈橢圓形欧美激情偷乱人伦小说专区，卵裂為盤狀卵裂；在卵囊內(nèi)鸥美色精品在线播放，胚胎發(fā)育包括卵裂期又污又黄又爽的网站、囊胚期、原腸胚成年人在线观看、膜內(nèi)擔輪幼蟲人人妻人人干、膜內(nèi)面盤幼蟲；幼蟲發(fā)育包括面盤幼蟲在线免费免费观观看看的黄色视频、后期面盤幼蟲和匍匐幼蟲人人插人人透人人射人人色人人干。

These observations show that reprogramming is easier in interspecific embryos reconstructed with ES cells than that reconstructed with somatic cells, and that ES cells have the higher ability to direct the reconstructed embryo development normally than fibroblast cells. Oocytes were reconstructed with outbreeding Kunming albino mouse ES cells and enucleated rabbit oocytes, and the effects of the passages of ES cells and 6-DMAP on the development of interspecific reconstructed oocytes were analyzed. The interspecific reconstructed ES-rabbit oocytes were activated either by combined two set electric pulses and 6-DMAP or by two set electr

以上結果顯示，6－DMAP能增加胚胎干細胞異種重構卵的卵裂率色黄视频污，對重構卵囊胚的發(fā)育率影響不大国产一在线精品自产拍；高培養(yǎng)代數(shù)和低培養(yǎng)代數(shù)的胚胎干細胞用于異種核移植不影響異種重構卵的卵裂率和囊胚發(fā)育率；用胚胎干細胞作為供核細胞91视频日韩；比體細胞作為供核細胞所構建的異種重構胚更容易進行重編程无码专区无码专区，并且胚胎于細胞指導異種克隆胚胎正常發(fā)育的能力強于體細胞。

The results show that FISH can be used in experimental study and clinical application of PGD; 3. The cause of multi- pronucleus in embryos from IVF or ICSI would be different.

IVF受精和 ICSI受精的3PN胚胎發(fā)育有差異亚洲久久青草鸭，證明＊F受精和 ICSI受精的3PN中的原核的來源是不同精品无码三级在线视频网站，導致胚胎第一次卵裂的方式也不同黄片九区域免费观看。

The result of frozen MⅡoocytes using three methods indcated that the self-restraintspear method was better than OPS, and the effection of frozen altogether with self-restraintspear method and centrifugal was the best; the frozen effection was not ideal using fluidspear for 8- cell ovocyte.

用三種冷凍方法(OPS法；自制移液槍頭法精品思思啪啪；移液槍頭法+離心法)對MⅡ卵母細胞冷凍的結果表明jizz女，自制移液槍頭法優(yōu)于OPS法，其中以移液槍頭法+離心組的冷凍效果最好日本性爱网；用移液槍頭法對體外發(fā)育到8-細胞期卵母細胞的冷凍效果不理想免费看污视频的网站。MⅡ期卵母細胞用OPS法冷凍后卵母細胞IVF和電激活的卵裂率都顯著高于移液槍頭法(P＜0.01)，電激活后2-細胞卵裂率比IVF高成熟丰满熟妇XXXXX图片。

In this study, the effects of STH and insulin on porcine in vitro oocyte maturation and cleavage after parthenogenetic activation were investigated.

研究了生長激素久久精品大全、胰島素單獨或者聯(lián)合作用對豬卵母細胞體外成熟及孤雌激活后卵裂的影響。

Leaves simple; petiole 1.5--4(--7) mm; leaf blade ovate or lanceolate in outline, pinnatipartite or pinnatisect, sometimes pinnatilobed, 0.5--5 × 0.3--3 cm, leathery, both surfaces sparsely puberulous or subglabrous, base cuneate or subtruncate; lobes in 1--3(or 4) pairs, undivided and linear or subrhombic and unequally 2- or 3-lobulate, 4--15 mm, apex acute; lobules ovate, triangular, or linear; midvein abaxially slightly prominent or flat, inconspicuous.

單葉 葉柄1.5-4(-7)毫米欧美性一级交视频；葉片卵形或披針形輪廓欧美中文一级A片在线播放，羽狀深裂或羽狀全裂，有時羽狀淺裂的强奷漂亮的护士中文字幕， 0.5-5 * 0.3-3 厘米国产目拍精品一区二区，革質(zhì)，兩面稀疏被微柔毛的或近無毛日韩精品一区在线播放视频，基部楔形或近截形人人插人人人人操人人爽；在對，不裂和線形或1-3(或4)內(nèi)裂片和不相等2或3具小裂片国产盗摄偷窥在线，4-15毫米初高中精品视频进入，先端銳尖；卵形97人人草,人人干，三角形的小裂片5566亚洲制服丝袜先锋，或線形；中脈的背面的稍突出或平中文字幕在线网，不顯眼欧美性爱操妣。

This experiment aimed to systematically investigate and analyze the ethanol activated rates of mouse oocytes, affected factors of oocytes activation, types and development of parthenotes, and the organization and roles of the cytoskeleton during pronuclear formation and migration and early embryo cleavage by ethanol activation of oocytes, embryo culture in vitro and immunofluorescence cyto-chemistry. The results:(1) The ethanol activation of mouse oocytes showed that the activated and developmental rates of oocytes increased significantly with the increase of ethanol concentration and extension of exposure time, but over concentration and exposure time would result in increased fragment rates significantly. 7% ethanol treated oocytes for 7min was the optimum activated condition.

本實驗主要利用卵母細胞的乙醇激活、胚胎體外培養(yǎng)和免疫熒光細胞化學方法對小鼠卵母細胞乙醇激活效率2021天天做夜夜爽网站、影響因素JIZZJIZZ亚洲日本少妇、孤雌胚類型、發(fā)育97超碰免费、原核形成、遷移及早期胚胎卵裂過程中細胞骨架的組裝99r在线观看视频精品、作用等進行了系統(tǒng)的研究和分析处女开破视频，結果顯示：(1)小鼠卵母細胞的乙醇激活結果表明，隨著乙醇濃度的升高和作用時間的延長亚洲欧洲第一区二区三区，卵母細胞激活率和發(fā)育率都顯著提高美国,国产欧美一区，但乙醇濃度過高和作用時間過長會導致卵母細胞碎裂率的顯著增加无码国产精品一区二区gif，7%乙醇作用卵母細胞7min 為最佳激活條件。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分為4個部分尤物国产一区二区三区，第一部分對影響豬卵母細胞體外成熟和孤雌激活后胚胎分裂的幾個重要條件進行了比較研究深夜女人18毛片A级毛片免费，確立了一種較好的培養(yǎng)方法：與顆粒細胞共培養(yǎng)，找到了一種適合豬卵母細胞體外成熟的培養(yǎng)基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸99riav在线一区，成熟率和分裂率分別為(86.7±3.35)%和(86.3±4.16)%A片看片资源，國外報道的最高成熟率為(85.7±4.1)%；第二部分對豬卵母細胞孤雌激活的化學方法進行了研究欧美视频一区二区视频在线，確立了化學激活豬卵母細胞的兩種最佳方法：1將成熟的去卵丘顆粒細胞的MII期卵母細胞用20μmol/Lionomycin作用30min亚洲一级免费网址，再將卵母細胞培養(yǎng)于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培養(yǎng)液中，卵裂率和桑囊胚發(fā)育率達到(76.7±7.6)%和(37.1±6.4)%2將成熟的去卵丘顆粒細胞的MII期卵母細胞在200μM/L的Thimerosal中處理20min亚洲色图一区二区，再與8mM的DTT共孵育30min人人爱人人爱人人操，卵裂率和桑/囊胚形成率為(81.0±2.8)%和(39.6±2.7)%；第三部分對孤雌激活胚胎的培養(yǎng)條件進行了研究欧美日韩国产免费午夜在线观看，確立了一種最佳的胚胎培養(yǎng)條件：在SOF簡單培養(yǎng)基中添加顆粒細胞進行前3天的培養(yǎng)久久一级片视频，然后轉入添加胎牛血清的NCSU—23培養(yǎng)基并和輸卵管上皮細胞進行后期的培養(yǎng)，其桑椹胚和囊胚的發(fā)育率為(59.5±3.2)%亚洲综合爱爱12p；第四部分研究了IGF-I

The sixth, there were no significantly differences between colchicine groupsand vinblastine groups on the metaphases, in the experiment of prepare mouse4-cell embryo stage single blastomere chromosome specimen by using optimumparameters; and there were significantly differences between colchicine groupsand vinblastine groups on the metaphases (P＜0.05), in the experiment of preparemouse 8-cell embryo stage single blastomere chromosome specimen by usingoptimum parameters.The end, the preparation quality of mouse 4-and 8-cell embryo stage singleblastomere specimen were 2.5, 2.7, 2.4, 1.9, respectively.

低滲液選用0.45％氯化鈉時免费视频精品一区二区三区，低滲效果優(yōu)于選用0.5％檸檬酸鈉和0.35％氯化鉀的。6直接在线免费看黄网站、在利用最佳參數(shù)制備小鼠4-細胞期胚胎單卵裂球染色體標本時美女被插出白浆，經(jīng)統(tǒng)計分析，兩種阻斷劑的作用效果差異不顯著爱爱网站永久免费；而在制備小鼠8-細胞期胚胎單卵裂球染色體時阻斷劑選用長春花堿的效果優(yōu)于秋水仙素的(P＜0.05)人人色人人操人人射。7、秋水仙素組和長春花堿組小鼠4女被操网站、8-細胞胚胎單卵裂球染色體標本制備質(zhì)量分別為：2.5性欧美另类、2.7、2.4欧美在线视频干美女、1.9亚洲天堂国产制服。