A independent life individuality is finally formated from singlecell ferti1ized egg throngh egg cleavage色秀视频久久久、morula stage国产乱人伦免费精品视频、blastocoele stageMm一级黄色、protogaster stage and orange etc, and concerned in major basal questions of development and gene expression.

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The repeated detection of single blastomere of untransferred embryos verified the PGD results, and further indicated that our PGD technique of ACH was relatively stable and reliable.

通過對未移植胚胎的單卵裂球的重復(fù)檢測，驗證了PGD的結(jié)果狠狠cao青青青视频，進(jìn)一步說明了我們開發(fā)的ACH的PGD技術(shù)是較穩(wěn)定一级无码午夜在线观看、可靠的。

One embryonic cell can be separated into many blastomere cells. As be the nuclear donor of the embryonic cell nuclear transfer, it can breed the number of eminent species largely, increase the number of endangered animals, supply large number of supply same genotype animals, improve the efficiency of gene transfer and sex control and offer same genotype apparatus or organ for human transplant.

一個早期胚胎細(xì)胞可以分離多個卵裂球久久偷拍九九99热，以此作為核供體進(jìn)行胚胎細(xì)胞核移植www.天天激情网.com，可以大量擴(kuò)繁優(yōu)良品種的數(shù)量，增加瀕危動物的數(shù)量无码一级午夜久久福利精品，為科學(xué)研究提供大量遺傳同質(zhì)的動物成年在线观看免费人视频，提高轉(zhuǎn)基因和性別控制技術(shù)的效率，提供基因型相同的器官或組織供人類移植久久久精品一区二。

Porcine was acted as the experimental animal. It was researched on problem of the origin of obtaining early embryonic cells, methods of separating blastomere cells, best parameter and order of ECNT embryo electric activation and system of ECNT embryo IVC, which was to find a scientific method of fitting ECNT and a procedure of fitting the lab producing embryos largely, optimize the procedure of ECNT and improve the ECNT embryo in vitro.

本實驗以豬為實驗動物第四色色色av，對豬胚胎細(xì)胞核移植供體的來源、卵裂球細(xì)胞分離方法、核移植重建胚的電激活最佳參數(shù)成人乱人伦精品影视大全在线观看视频、核移植受體的激活時序以及核移植重建胚體外培養(yǎng)系統(tǒng)相關(guān)問題進(jìn)行了研究品爱网尤物视频，旨在探索一種適用于豬胚胎細(xì)胞核移植的科學(xué)方法，提高豬胚胎細(xì)胞核移植胚胎的體外發(fā)育率日韩成熟熟女精品自拍视频，優(yōu)化胚胎細(xì)胞核移植程序久久综合激情综合五月丁香。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cytoplasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methylation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribution of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

結(jié)果表明：利用電融合方法制備的小鼠四倍體胚胎在體外培養(yǎng)體系中經(jīng)歷細(xì)胞質(zhì)融合、細(xì)胞核融合及細(xì)胞繼續(xù)分裂發(fā)育直到囊胚期的過程欧美一级片电影在线观看，在細(xì)胞質(zhì)融合的時候胚胎卵裂球同體內(nèi)體外培養(yǎng)二倍體胚胎一樣18禁网站在线永久免费观看，呈現(xiàn)高度甲基化狀態(tài)；在細(xì)胞核開始融合的時候色哟最新网站入口，甲基化水平急速下降久久久久久久久久精品一，在細(xì)胞核完全融合的時候甲基化水平達(dá)到最低點；隨著胚胎繼續(xù)分裂日韩精品更新影院在线播放视频，胚胎甲基化水平逐漸增加女人扒开腿让男人桶爽69视频，在桑葚胚期甲基化水平最高；但是囊胚期四倍體胚胎內(nèi)細(xì)胞團(tuán)同滋養(yǎng)層細(xì)胞甲基化熒光信號沒有差別看黄网站免费中文字幕，這與體內(nèi)體外培養(yǎng)二倍體囊胚內(nèi)細(xì)胞團(tuán)細(xì)胞甲基化熒光強(qiáng)度高于滋養(yǎng)層細(xì)胞甲基化熒光強(qiáng)度不同www.色中射.com。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastocysts followed by fusion of cyto-plasm and nucleus and cleavage in vitro. During the fusion of cytoplasm, the DNA methylation levels of the fused embryos are as high as these of two-cell diploid embryos in vivo Then the embryos are rapidly demethylated when the nucleus begin to fuse, resulting in the lowest DNA methylation levels when the nucleus are fused completely. After that, the DNA methy-lation levels of the fused embryos are gradually increased until the morula stage. However, whereas an asymmetric distribu-tion of DNA methylation is established in vivo-derived blastocysts with a higher methylation level in the inner cell mass than that in the trophectoderm, we can not detect the asymmetric distribution in most in vitro-derived tetraploid blastocysts.

結(jié)果表明：利用電融合方法制備的小鼠四倍體胚胎在體外培養(yǎng)體系中經(jīng)歷細(xì)胞質(zhì)融合、細(xì)胞核融合及細(xì)胞繼續(xù)分裂發(fā)育直到囊胚期的過程黄色网站在线观看网址，在細(xì)胞質(zhì)融合的時候胚胎卵裂球同體內(nèi)體外培養(yǎng)二倍體胚胎一樣欧美影音先锋，呈現(xiàn)高度甲基化狀態(tài)；在細(xì)胞核開始融合的時候国内自拍骚视频，甲基化水平急速下降欧美性爱一级黄色网站，在細(xì)胞核完全融合的時候甲基化水平達(dá)到最低點；隨著胚胎繼續(xù)分裂欧美成人大片在线，胚胎甲基化水平逐漸增加91自拍视频精品，在桑葚胚期甲基化水平最高；但是囊胚期四倍體胚胎內(nèi)細(xì)胞團(tuán)同滋養(yǎng)層細(xì)胞甲基化熒光信號沒有差別国产99视频精品免视看9，這與體內(nèi)體外培養(yǎng)二倍體囊胚內(nèi)細(xì)胞團(tuán)細(xì)胞甲基化熒光強(qiáng)度高于滋養(yǎng)層細(xì)胞甲基化熒光強(qiáng)度不同香蕉视频久。

Tetraploid embryos could be produced by electrofusion at the stage of two-cell embryos, which could develop to blastcysts fellowed by fusion of cytoplasm and nucleus and cleavage in vitro.After fusion of cytoplasm, the DNA methylation levels of the fused embryos was very high as well as two-cell diploid embryos in vivo.Then the embryos was rapiddly demethylated when the nucleus begin to fuse, resulting the lowest DNA methylation levels when the nucleus fused completely.After that, the DNA methylation levels of fused embryos were gradually increased until the blastocysts stage.However, whereas an asymmetric distribution of DNA methylation was established in an vivo-derived blastocysts with a higher methylation level in the inner cell mass than in the trophectoderm, in most vitro-derived tetraploid blastocysts, we can not detect the asymmetric distribution.

結(jié)果表明：利用電融合方法制備的小鼠四倍體胚胎在體外培養(yǎng)體系中經(jīng)歷細(xì)胞質(zhì)融合、細(xì)胞核融合及細(xì)胞繼續(xù)分裂發(fā)育直到囊胚期的過程日本巨乳在线，在細(xì)胞質(zhì)融合的時候胚胎卵裂球同體內(nèi)體外培養(yǎng)二倍體胚胎一樣在线黄黄网站，呈現(xiàn)高度甲基化狀態(tài)；在細(xì)胞核開始融合的時候自拍偷拍一区二区，甲基化水平急速下降www.sy897.com，在細(xì)胞核完全融合的時候甲基化水平達(dá)到最低點；隨著胚胎繼續(xù)分裂天堂网wwwww，胚胎甲基化水平逐漸增加亚洲日本www，在囊胚期甲基化水平最高；但是囊胚期四倍體胚胎內(nèi)細(xì)胞團(tuán)同滋養(yǎng)層細(xì)胞甲基化熒光信號沒有差別，這與體內(nèi)體外培養(yǎng)二倍體囊胚內(nèi)細(xì)胞團(tuán)細(xì)胞甲基化熒光強(qiáng)度高于滋養(yǎng)層細(xì)胞甲基化熒光強(qiáng)度不同国产在线观看小视频。

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Radical leaves: stipules ovate, herbaceous, adnate to petiole; petiole sheathing at base; leaf blade imparipinnate; leaflets opposite, rarely alternate, broadly elliptic or obovate-oblong; cauline leaves few, with few leaflets, or simple and then margin 3-lobed or 2- or 3-serrate.

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Flowering stems erect, 20-40 cm. Proximal stem leaves 3- or 4-verticillate or opposite, sessile,± lanceolate- to ovate-oblong, linear-oblong distally on stem, 2-5 × 0.6-1 cm, margin remotely and coarsely dentate, pinnately lobate, or subentire.

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