We studied the optimizaiton, accuracy and reliability of this technique by definition the genotype of five common mutation loci of β-globin gene in the carrier with CD41-42 mutation and that of β-globin gene of normal people's single lymphocyte and single blastomere.

我們采用了一種利用單細(xì)胞標(biāo)本通過(guò)二重巢式PCR并結(jié)合ARMS技術(shù)確定中國(guó)人群中廣泛的β-地中海貧血突變譜的基因型的策略国产视频免费视频在线，通過(guò)對(duì)含有β-珠蛋白基因CD41-42突變的攜帶者5個(gè)該基因常見(jiàn)突變位點(diǎn)基因型的確定97人伦人妻对对碰免费看，以及正常人的單個(gè)淋巴細(xì)胞欧美性爱综合肏、單卵裂球的β-珠蛋白基因該5個(gè)位點(diǎn)基因型的確定人人看人操，對(duì)這種技術(shù)條件的優(yōu)化、準(zhǔn)確性和可靠性進(jìn)行了研究亚洲欧美啊啊啊啊久久久。

Following 72 h co-culture, desquamate, sporozoites, trophozoites, meronts, microgametocytes, macrogametocytes, zygote, thin-wall oocyst, and thick-wall oocyst appeared orderly. Between the 60th and 72th hour, many oocysts emerged. Inoculated by the C. parvum -infected cell culture supernatant at the 48th hour, the immunosuppressed mice became infected.

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Leaves 5, not fully expanded at anthesis; petiole 5.5--15 cm, with thick long pilose hairs; leaf blade nearly kidney-shaped, rounded-ovate, or 5-angled, 2.5--7 × 4.5--11 cm,± equally 3-foliolate, abaxially with white hairs, adaxially glabrous, base broadly cordate; lateral leaflets subsessile, unevenly 2-lobed; central leaflet subsessile or with a 1--4 mm petiolule, deeply 3-lobed, lobes entire or dissected, ultimate lobes triangular to narrowly linear and 2--3.5 mm wide.

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Female flowers solitary or several and male mixed in axillary cluster; pedicels 2-6 cm; calyx yellow-green, 6-lobed; sepals slightly thick, biseriate, outer obovate or subspatulate, ovate-elliptic, 6-7 × 3-4 mm, inner shorter, obovate, ca. 5 mm, with purple stripes; disk absent; pistil depressed globose, ca. 2 × 2.7 mm, 3-locular; styles 3, bifid at apex, arms recurved.

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Basal leaves 2 or 3; petiole 7--20 cm, base narrowly sheathed; leaf blade dark green when dried, pentagonal, 2.6--5.5 × 4.8--11 cm, base deeply cordate, 3-parted; central lobe rhombic-ovate or rhombic, 3-fid, incised-lobulate or dentate at margin; lateral lobes obliquely flabellate, unequally 2-fid.

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Basal leaves 2-6, long petiolate; stipules over 1/2 adnate to petioles, broadly lanceolate, membranous, margin sparsely denticulate and ciliate or entire, apex acuminate; petioles 3-9 cm at anthesis, usually green, sometimes purplish, glabrous, nitid, elongated at fruiting to more than 20 cm; leaf blade glabrous on both surfaces or pubescent along veins, 3-sect, segments shortly stipitate, lateral segments deeply 2-fid, central segment deeply 2- or 3-fid, terminal lobes highly variable in shape and size, ovate-lanceolate, lanceolate, oblong, or linear-lanceolate, margin irregularly dentate or shallowly divided, sometimes triparted to pinnatiparted or 3-lobed, apex obtuse or acute.

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Basal leaves 5--10; petiole 2--6 cm; blade ternate, or sometimes simple, pentagonal or broadly ovate in outline, 0.6--1.5 × 1--2.4 cm, thinly papery, subglabrous or abaxially puberulent; leaflets petiolulate, rhombic, 2- or 3-lobed or 2- or 3-partite, sometimes 1--2 × 3-sect and with ultimate segments lanceolate-linear; central lobe rhombic-obovate, 3-lobed or 3-partite.

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Leaves basal and on stem, opposite, rarely in whorls of 3, lanceolate to ovate, pinnatifid to pinnatisect.

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Resembled to control oocytes, MAPK was inactivated after cytoplasts activation. However, after activation the cytoplasts showed fragmentation while pronuclei were formed in the control oocytes. This indicated that nuclei play important role in maintainence of oocytes normal morphology.

成熟胞質(zhì)的活化與卵母細(xì)胞相似亚洲精品欧美激情专区，在活化后MAPK被迅速滅活未满进入的黄色网站，只是胞質(zhì)在活化處理后均發(fā)生碎裂，而卵母細(xì)胞活化后形成原核禁止18以下观看在线网站，這表明核在細(xì)胞形態(tài)維持中起重要作用一级作爱视频无遮拦。

Basal leaf blade 6-15 mm, broadly obovate, broadly ovate, or broadly rhomboid-ovate; staminodes 2-lobed, posterior lobes 2- or 3-lobed

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