It showed that the cumulus cells did play significant role in the stimulation of nuclear maturation of porcine oocytes and subsequent development.

表明卵丘細胞對豬卵母細胞的核成熟及卵母細胞體外受精后的卵裂和早期胚胎發(fā)育99精品热视频只有精品16，都具有明顯的促進作用免费一级a片在线播放视频。

The experiment results were as following:All cytoplast recipients derived from oocytes of rabbit, bovine and goat can support early development of cloned embryo, the fusion rate of reconstructed embryos of goat-bovine and goat-rabbit were 59.91% and 74.10%, their cleaved rate were 57.48% and 72.57%, blastocyst rate were 9.59% and18.06% respectively, and the fusion of reconstructed embryos of goat-rabbit and goat-goat were 60.01% and 74.10%, their cleaved rate were 56.68% and 72.57%, blastocyst rate were 11.76% and18.06% respectively.

試驗獲得了如下結(jié)果：1国产在线观看三级。以山羊耳成纖維細胞為供體操美屄不卡黄色网站，以牛、羊卡通动漫亚洲综合第21页、兔卵母細胞做為胞質(zhì)受體構(gòu)建重構(gòu)胚99re在线视频免费观看精品，并經(jīng)體外培養(yǎng)，發(fā)現(xiàn)不同的胞質(zhì)受體均能支持重構(gòu)胚的早期發(fā)育免看一级a一级久久，但受體胞質(zhì)對重構(gòu)卵的重構(gòu)胚早期發(fā)育速度和卵裂率均有顯著影響在线免费自拍视频。

Stigma 2-cleft. Fruit indehiscent, small, obovoid to globose, included or nearly included in calyx, 1-(or 2)-seeded.

柱頭2半裂。果不裂在线无码高清不卡，小免费麻豆精品无码国产在线，倒卵球形的到球形，包括或差不多內(nèi)藏于花萼国产九九久久精品视频，1-(或2)種子三级黄免费视频网站。

Capsules baccate, ovoid or subglobose, 2-2.5 × 1.5-2 cm, red-yellow to purple when mature, indehiscent, or 3-valved when dry; arils white.

蒴果漿果狀，卵形體或近球形美女被操黄片， 2-2.5 * 1.5-2 厘米欧美视频黄页，成熟時紅色黃到紫色，不裂在看看的网址你懂的，或干燥時3瓣裂的99热思思在线国产；假種皮白色。

The cleavation rates and blastocyst development rates of cloned embryos were used to assess the efficiency of different operational procedure. Finally, the best combination of operational procedure, that the spindle-viewer system was used for oocytes enucleating, and donor cell was electrofused into ooplasm by electrical pulse (1.9 kV/cm, 10 ms, two) to reconstruct bovine cloned embryos.

以核移植胚胎的卵裂率日韩妓女精品影院视频在线、囊胚發(fā)育率作為檢測指標，對不同的方法所獲得的克隆胚胎的卵分裂率與囊胚發(fā)育率進行比較91在线国内在线自产，最后篩選獲得1個優(yōu)化的牛體細胞核移植操作程序先锋资源在线看，即采用Spindle view系統(tǒng)對牛卵母細胞進行去核操作，將供核體細胞注射到卵周隙欧美日韩亚洲，然后通過電融合法將供體核引入去核卵細胞質(zhì)(電融合參數(shù)為1.9 kV/cm超碰图片，脈沖時程10 ms，方波2次間隔2 s)色哟哟视频网。

Analysis of mitochondrial DNA composition of the panda-rabit cloned embryos found that mitochondria from both panda somatic cells and rabbit ooplasm co-existed in early blastocyst, but mitochondria from rabbit ooplasm decreased and those from panda donor cells dominated in early fetuses after implantation.

結(jié)果顯示欧美A性交，應用卵裂球電融合方法可以制作一系列的多倍體胚胎；小鼠囊胚的形成與胚胎中的細胞數(shù)目無直接關(guān)系国产色区福利，卵裂球的電融合和染色體數(shù)目的增加不會改變胚胎的發(fā)育進程黄色网站在线观看18；而胚胎細胞中的核／質(zhì)比可能是控制胚胎囊胚化的一個重要因素。

The cleavage rate of ovocyte IVF of MⅡovocyte frozen withOPS method and activation method was higher remarkably than fluid spearmethod(P＜0.01), the cleavage rate of 2-cell after electrical activation was higher than IVF.

本試驗推薦豬體外成熟培養(yǎng)體系為連續(xù)培養(yǎng)液48h的NCSU-23+10％FBS免费在线观看在线黄色视频，細胞濃度為100個／400μl在线观看精品国产福利AV，發(fā)育培養(yǎng)體系為NCSU-23+BSA日韩毛片无码一级观看免费；用CRY-3細胞激活儀電激活時(電融槽寬度為1mm)為150v／20μs／2次+600μMγ-BLI；以活力為0.1左右的冷凍精液進行IVF時人人弄人人玩人人搡，精子濃度為10~7天天干网，共孵時間為6h，并在成熟處理時只部分去除卵丘細胞亚洲哟哟精品网站；MⅡ卵母細胞冷凍時亚洲产国偷v产偷v自拍小说，方法為移液槍頭法+離心法，冷凍后激活的2-細胞卵裂率比IVF高在线超碰观看。

We biopsied 1-2 single blastomere from 6-8 cell cleavage stage intracytoplasmic sperm injection embryo, and using nested PCR amplified the high frequently mutation region G380R of FGFR3 gene of the single blastomere. The products of PCR were digested by restriction enzyme Bfm Ⅰ, then the digested products were detected by 10% PAGE to see whether the embryo inherted the mutation of the patient and to screen out normal embryo transfer.

活檢經(jīng)胞質(zhì)內(nèi)單精子注射(intracytoplasmic sperm injection插逼吃奶头69黄色视频，ICSI)授精的胚胎發(fā)育至6～8細胞期的1～2個單卵裂球，采用巢式PCR擴增單卵裂球的FGFR3基因的高發(fā)突變位點G380R區(qū)域男女八x八x视频网站，用限制性內(nèi)切酶BfmⅠ消化巢式PCR的內(nèi)擴增產(chǎn)物国产999国内精品视频免费，再經(jīng)10%聚丙烯酰胺凝膠電泳檢測有無遺傳患者的該種突變，從而篩選出正常胚胎移植看黄免费看。

5 Mm. Female flowers: pedicels short but extending to ca. 5 mm in fruit; sepals 5, oblong-ovate to ovate-lanceolate, ca. 8 mm; petals and disk as in male; ovary ovoid; styles 3, apex bifid.

雌花：花梗短日韩一级aa大片免费视频，但在果期延伸到約5毫米；萼片5欧美日韩综合自拍小说网站，長圓狀卵形的到卵狀披針形国产网站自拍，約8毫米；如同在男性里的花瓣和花盤黄免费视频色；子房卵球形91精品久久久久久中文字幕无码；花柱3，先端2裂美女视频网站一区。

Methods and Results Experiment 1: Intracytoplasmic injection of rat sperm heads was performed using 7~10 μm and 2~4 μm diameter pipettes and different injected method respectively.

方法和結(jié)果實驗1：用直徑為7~10μm和2~4μm的注射針以及相應的注射方法進行大鼠ICSI538再线视频，ICSI后卵母細胞存活率(30.5% vs。 61.3%)和卵裂率(12.5% vs美女被啪啪网站。 51.1%)均差異顯著(P.05)激情视频毛片在线观看；實驗2：分別在hCG后14 h、16 h和18 h取卵進行ICSI久久久久久精品欧美，三組存活率77.4%A级国产国偷自产在线观看、74.1% vs。