Results (1) The forward and reverse subtracted cDNA libraries of different metastastic potential large cell lung cancer cell lines were successfully constructed;(2) With blue-white colony and dot blot, 307 positive clones in the forward subtracted library and 78 positive clones in the reverse subtracted library were obtained;(3) 55 clones were successfully sequenced in the forward subtracted library. Homolog analysis confirmed 23 differential expression segments, which were similar to the human genes already known, including NME2, NPM1, MT 2A, HSPE1, TAFIA, EPRS, PX19 and EIF3S9 et al;(4) 31 clones were successfully sequenced in the reverse subtracted library. Homolog analysis confirmed 16 differentially expressed segments. 15 of them were similar to the human genes already known, including ANXA2, TUBB, PKN2, GNAS, EEF1A1, SSR2 and RPLPO et al. Only one segment had partial homology to known human genes. This segment was supposed to be the new EST segments which might not have been cloned.

結(jié)果(1)成功構(gòu)建人大細(xì)胞肺癌高低轉(zhuǎn)移株差異表達(dá)基因正向消減cDNA文庫和反向消減cDNA文庫亚洲精品区第3页；(2)經(jīng)藍(lán)白菌落篩選和斑點(diǎn)雜交，正向消減文庫獲得307個(gè)陽性克隆，反向消減文庫獲得78個(gè)陽性克隆亚洲精品电影你懂的在线观看；(3)正向消減文庫挑選55個(gè)克隆成功測序国产精品无码AV在一区，經(jīng)同源性分析最終確定差異表達(dá)基因片段23個(gè)與已知人類全長基因具有很高相似性(95％～100％)国产在线无码精彩视频，這些基因包括NME2、NPM1先锋影音亚洲中文字幕5566、MT2A毛片一级A片免费、HSPE1、TAFlA午夜免费播放成人无码电影视频、EPRS亚洲线精品一区二区三区在线观看、PX19和EIF3S9等；(4)反向消減文庫挑選31個(gè)克隆成功測序一级黄色视频在线免费播放A级，經(jīng)同源性檢索和比對最終確定差異表達(dá)基因片段16個(gè)曰本一级AAA片免费，其中15個(gè)與人類全長基因具有很高相似性(95％～100％)，包括ANXA2午夜网站加勒比我污、TUBB国产黄在线视频、PKN2、GNAS欧美精品午夜理论片在线播放情事、EEF1A1豪妇荡乳1一5潘金莲、SSR2和RPLPO等基因。1個(gè)片段和己知人類基因僅有部分相似性黄色网站免费进入入口，表明它可能是未被克隆的人類基因EST片段亚洲区四区。

They were classified into CYP86 family for over 38% identities of the amino acid sequences of the three genes to CYP86 genes from Arabidopsis thalia, whereas BcCYP86MF and RsCYP86MF were sorted into CYP86C4 subfamily in terms of over 55% similarities to CYP86C4 at the amino acid level, and OvCYP86MF into CYP86C3 subfamily according to 86.5% homology between them at the amino acid level.

BcCYP86MF和RsCYP86MF與CYP86C4亞家族氨基酸同源性均大于55%激情人妻一级毛片在线视频，則可歸為CYP86C4亞家族，而OvCYP86MF與CYP86C3的同源性最高毛片网在线观看，為86.5%不卡一级片，應(yīng)歸為CYP86C3亞家族。

The cloned BBE gene sequences were 94.84% identified with the reported BBE genes in GenBank previously. Based on the cDNA sequences of COR and BBE ,two fragments about 400～500 bp from each gene with lower identity among them were cloned. The fusion gene BC (744 bp) is fused by the PCR technique. Then the promoter CaMV 35S driven, containing'forward BC fusion fragments-reverse pdk intron-reverse BC fusion fragments', plant siRNA expression vector were constructed based on the vectors pHANNIBAL and pART27.The work will lay the foundation for breeding a low morphine and high thebaine poppy.

利用blast及分子生物學(xué)軟件DNAStar對COR和BBE基因的cDNA序列同源性進(jìn)行分析比較久久国产91视频，分別從各基因中篩選和克隆了一段同源性極低在线观看操比黄片视频、約400～500 bp的片段；并應(yīng)用重疊PCR法將其拼接成744 bp的融合基因BC欧美黄色网址免费，以中間載體pHANNIBAL和植物表達(dá)載體pART27為基礎(chǔ)a4yy一级午夜福利，構(gòu)建了以CaMV 35S啟動子驅(qū)動的含有"正向BC融合片段- pdk內(nèi)含子-反向BC融合片段"的ihRNAi植物表達(dá)載體，通過轉(zhuǎn)化野生罌粟黄色视频精品观看，初步研究了以COR和BBE基因?yàn)榘袠?biāo)的RNAi對內(nèi)源嗎啡合成的抑制效果日韩精品专区在线播放免费，為進(jìn)一步培育低嗎啡高蒂巴因的罌粟種質(zhì)提供了依據(jù)。

OPJ16-759 shows 83% to 100% identity with 14 EST sequences from abiotic stressed leaves of Vitis vinifera var. Chardonnay, 94% to 100% identity with 3 EST sequences from water stressed berry of Vitis vinifera var. Cabsau.

OPJ16-759與歐洲葡萄"霞多麗"葉片14條非生物脅迫EST序列有83 ~ 100%的同源性日高清无码视频，與歐洲葡萄"Cabsau"漿果3條水分脅迫的EST序列有94 ~ 100%的同源性人妻系列爽爽无码专区久久69堂。

The both amino acid sequences contain 7 WD domains and their high identities are up to 99%.

兩者的氨基酸序列均具有7個(gè)WD結(jié)構(gòu)域，且具有高度的同源性精品国产91亚洲，同源性高達(dá)99%www.色色色五月天。

Sequencing and homology analysis revealed that the gene fragment was 395 bp in length and shared up to 89% similarity with NB gene from winter squash. The deduced amino acid sequence for the fragment was composed of 131 amino acid residues.

經(jīng)過測序和同源性分析表明：該基因片段長度為395bp，與GenBank上所登錄的筍瓜NR基因同源性達(dá)89%日韩精品第十八页在线播放免费观看，根據(jù)所測序列推導(dǎo)該片段由131個(gè)氨基酸殘基組成久久久久精品无码AⅤ。

The predicated gene products of amrA, amrB showed high similarities with the typical response regulator, such as afsQ1, mtrB and ompR, about 40%-62%.

對它們的氨基酸序列分析表明：amkA和amkB與典型的組氨酸激酶，包括afsQ2精品二区视频在线观看，mtrB以及phoR欧美亚洲国产一区二区三区VA，有較好的同源性，大約為30％-44％性生活口交黄片；amrA和amrB與典型的反應(yīng)調(diào)節(jié)物一级一级一级一级一级片，包括afsQ1，mtrA和ompR等欧美激情精品视频一区二区，同源性很高国产自产拍一区精品，大約為40％-62％。

The comparison of sequences showed that the determined sequence was most similar to those in GenBank (AF016546)(similarity level, 97.9%), Phylogenetic analysis indicated that the resulting sequence was more similar to those of Mycoplasma spp.(homo!ogy level, about 70%), but was farther from Anaplasma spp.( homology level, about 50%).

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According to sequence cluster analysis of the the tested samples, it indicates that the gene distance of RKN-9, RKN-8, RKN-21, RKN-11, RKN-18 , RKN-20, RKN-17, RKN-22, RKN-23, RKN-24 and RKN-25 to Meloidogyne incognita and Meloidogyne arenaria was very close with homology of 100% and 99%, respectively.

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The cDNA document of ACC oxidase gene of Citrus aurantium L. was cloned. By using the program of BLAST on NCBI GenBank database, the sequence presented a very high match with the ACC oxidase genes from other plants. The base sequence was analysed by using biology programe of DNAStar 5.0. 278 amino acids were coded by the base sequence and the base sequence had the same conserve region of ACC oxidase gene of many kinds of other plants. The base sequences comparability was more than 72% compared with those of many kinds of other plants. The amino acid sequences comparability was more than 70% compared with those of a lot of other plants.

克隆了酸橙1-氨基環(huán)丙烷－1-羥酸氧化酶基因cDNA片段，將片段序列在NCBI網(wǎng)站上進(jìn)行同源性搜索398AV影视，顯示的皆為不同植物的ACC氧化酶基因日本免费一区二区小视频，因而認(rèn)為所克隆的片段就是酸橙ACC氧化酶基因；并運(yùn)用DNAStar5.0軟件進(jìn)行序列分析国产一级一片免费播放放，推導(dǎo)的氨基酸序列為278個(gè)殘基欧美一区的网站在线观看；具有所有植物ACC氧化酶基因共有的保守區(qū)域；與多種植物的ACC氧化酶基因的核苷酸和氨基酸序列的同源性都在72%和70%以上国产免费午夜福利院网孕。