Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

將PCR產(chǎn)物進(jìn)行重組日韩三级电影网站，并將陽性重組質(zhì)粒，應(yīng)用M13測(cè)序系統(tǒng)對(duì)產(chǎn)物進(jìn)行DNA序列分析亚洲综合综合网，并與MPM-129株P(guān)1基因核苷酸進(jìn)行同源性比較福利一区在线清纯，發(fā)現(xiàn)有4個(gè)位置的堿基發(fā)生了變化，其同源性為98.8％亚洲狠狠综合网站，證實(shí)了PCR所擴(kuò)增DNA片段的準(zhǔn)確性和特異性人人爽人人爱人人干人人摸，同時(shí)也證實(shí)了不同MP組型的P1基因即使在保守區(qū)也存在著一定的差異，將克隆的目的DNA片段用異羥基洋地黃毒苷配基用隨機(jī)引物法標(biāo)記制備MP DNA探針久久免费妇女高潮看片A片，雜交后用堿性磷酸酶標(biāo)記的抗Dig多克隆抗體與雜交體反應(yīng)就爱干人干人操，再用BCIP和NBT呈色，制備MP DNA探針亚洲爱爱小视频，鑒定所擴(kuò)增片段的特異性色哟哟无码一区二区，與同位素探針比較，Dig探針不受半衰期限制精品日韩中文字幕，可反復(fù)使用亚洲网在线，而且價(jià)格低廉，值得推廣使用一级a毛片水多视频国产一。

Bicolor genome showed higher homologous in repeat sequence than S. propinquum with S. dochna.

栽培高粱與擬高粱基因組間重復(fù)序列的同源性要比甜高粱與擬高粱間重復(fù)序列的同源性高三级网站午夜三级60分。

Rates of nucleotide substitution vary greatly among plant mitochondrial, chloroplast, and nuclear DNAs. Proc Natl Acad Sci USA ［J］.1987, 84 (5):9054.［3］ Ainouche M L, Bayer R. On the origins of the tetraploid Bromus species: insights from internal transcribed spacer sequences of nuclear ribosomal DNA［J］.

而山西平遙產(chǎn)山藥D= 0.004 76與山西太谷產(chǎn)山藥D= 0.004 27基本沒有差異，它們和河北安國(guó)產(chǎn)山藥D= 0.004 36共同與懷山藥有較大的同源性www.xxxxx，通過ITS序列分析国产区一区二区三区性色，這幾種不同產(chǎn)地的山藥之間堿基僅相差幾個(gè)，提示它們具有同源性亚洲桃色AV无码；野生山藥與種植山藥堿基數(shù)目相差較大欧美性交片视频，說明種植山藥在馴化過程中產(chǎn)生了變移，使物種更進(jìn)化先锋久久中文。

The comparison of these fragment sequences with those of other animalsthrough blastn of GeneBank,the results indicated that the homologies of nucleotidesequence of the GH gene of yak with Bos taurus are 98%,and with Bubalus bubalis,Capra hircus,Ovis aries are 97%,94%,93%,respectively.

其中牦牛GH基因全序列與普通牛同源性為98％，與水牛偷自拍另类亚洲清纯唯美、山羊爱爱视频在哪里考、綿羊同源性分別為97％、94％久久性生活视频免费、93％黑人一级婬片A片免费无码。

The IL-2 gene is 468bp, encoding a putative 155 amino acid protein, the DNA sepuencehomology of this caprine IL-2 and the corresponding caprine(AF535145) cytokine is 100.0％, thegene homology to Qihua Yings is 99.1％, the first 20 amino terminal aa sequence compose ahydrophobic signal sequence.

經(jīng)SignalP 3.0分析表明，前23個(gè)氨基酸為信號(hào)肽序列秋霞人成午夜免费大片麻豆；IL-2基因開放閱讀框架共有468bp古装观看一级古装A片，編碼一條155個(gè)氨基酸的多肽超碰碰人人摸97，與GenBank發(fā)表的山羊白細(xì)胞介素-2基因序列(AF535145)比較核苷酸／氨基酸同源性為100.0％，與應(yīng)琦華等的山羊IL-2序列的同源性為99.1％网站欧美黄色免费，前20個(gè)氨基酸為該蛋白的信號(hào)肽序列亚洲中文久久精品无码。

KDa. Phylogenetic tree analysis showed that the putative rice OsCAS had a high amino acid identity (99%) to maize β-caryophyllene synthase, whereas a low amino acid identity (51%) to those in other plants including Arabodopsis, Artemisia and cucumber.

系統(tǒng)進(jìn)化樹分析表明，水稻OsCAS基因編碼蛋白的氨基酸序列與同為單子葉植物玉米的β-石竹烯合成酶氨基酸序列同源性達(dá)99%wwwwww.日本，而與其他植物的同源性僅51%久久久精品中文字幕加勒比。

A first embodiment comprises a first common primer sequence, a first template homology region, a cleavable site, a second common primer sequence and a second template homology region.

第一個(gè)實(shí)施方案包含第一通用引物序列、第一模板同源性區(qū)多人群p视频免费观看、可切割位點(diǎn)在线网址免费观看av、第二通用引物序列和第二模板同源性區(qū)。

OsCH2 protein in rice has 85%,57%,57%,60%, 58% and 61% homologous withzea, gentiana, coffea, lycopersicon, citrus and crocus respectively. OsHSP82 protein has 95%,94%,90%,90% and 89% homologous with wheat,barley, tobacco,tomato and arabidopsisrespectively.

水稻OsCH2蛋白與玉米思思热99视频、龍膽草亚洲一区二区三区四区五区免费在线观看视频、阿拉比卡咖啡豆、番茄人人在线人人操九、甜橙粗暴大黑鳮巴视频欧美图片、番紅花蛋白同源性分別為85％、57％人人干人人操国产、57％99久久九九国产精品、60％、58％和61％日韩A片一级无码专区网址，OsHSP82蛋白與小麥饥渴熟妇高潮喷水、大麥、煙草又大又硬又粗视频网站免费观看、番茄和擬南芥蛋白同源性分別為95％美女污疼视频网站、94％、90％欧美性免费、90％和89％黄色在线网站视频。

Strains YL001 and YL002 formed a monophyletic clade with strains of X. nematophilus with sequence homology outweighing 99% and the sequence homology to genus Photorhabdus outweighing 94%. The lag, logarithmic, stationary and contabescence phase of the two bacteria were 0-6, 6-18, 18-66 and 66 h respectively; pH of strains YL001 and YL002 reduced to 5.70 and 5.56 at 12 h, respectively, and then rose gradually to 7.74 and 8.07 respectively when culture finished. Glucose content reduced quickly during 0-18 h, and then kept stable. Amido nitrogen content reached the lowest at 12 h and then rose slowly. YL001 exhibited the highest inhibitory effects at 54 h and YL002 exhibited highest inhibitory effects at 42 and 66 h respectively on B. cirerea and B. subtilis. Strains YL001 and YL002 belonged to X.

YL001和YL002菌株與嗜線蟲致病桿菌種內(nèi)菌株形成一個(gè)類群，序列同源性大于99%A性视频AAA，與發(fā)光桿菌屬內(nèi)菌株的序列同源性大于94%日韩精品视频热线在线观看。2菌株的延緩期、對(duì)數(shù)生長(zhǎng)期www.噜噜噜、穩(wěn)定期和衰亡期分別為0~699看黄，6~18，18~66和66 h老妇女棚户区卖淫对白视频；培養(yǎng)12 h后红牛黄色视频，YL001和YL002菌株發(fā)酵液的pH值分別降低至5.70和5.56，此后逐漸上升无码永久免费网站三级片，至發(fā)酵結(jié)束時(shí)其pH值分別為7.74和8.07男女干事网站；培養(yǎng)0~18 h時(shí)2菌株發(fā)酵液中還原糖含量迅速降低日日摸夜夜摸人人看亚洲综合，此后保持穩(wěn)定；12 h時(shí)氨基氮含量達(dá)到最低www.亚洲综合，此后開始緩慢上升大线香蕉在线视频网站观看；YL001菌株培養(yǎng)54 h后及YL002菌株培養(yǎng)42和66 h后，其對(duì)番茄灰霉病菌和枯草芽孢桿菌的抑制作用最強(qiáng)看片a免费观看视频大片。

The homologous comparison of the deduced amino acid sequence with other plant EPSP synthase proteins shows the identity to those of Fagus sylvatica, Oryza sativa Athaliana, phasedus vulgaris L respectively are 81%华人在线精品免费观看、81%、76%国产原创在线观看网站免费下载、74%.

同源比較該段序列所編碼的氨基酸序列可以發(fā)現(xiàn)EPSP合成酶有較高的保守性日韩欧美性交，與歐洲山毛櫸、水稻色五月亚洲AⅤ综合在线观看、擬南芥免费性生活福利视频、菜豆的同源性分別為81%、81%国广一级黄色网站、76%福利视频91、74%。