CEVd is a non-polyadenylated RNA viroid,but there is a fragment rich in A in CEVd sequence.On the bases of uniplex RT-PCR, a multiplex RT-PCR was developed to detect CTV,CEVd and CTLV simultaneously.

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The pHybLex/Zeo-Idl plasmid and the cDNA library plasmid were sequentially transformed into the yeast swains and screened to obtain Leu2^＋ and Leu2^＋LacZ^＋ clones, with the false positive clones excluded using positive and negative controls, and the plasmid of the true positive clone was sequenced and blasted for homological analysis.

方法PCR方法擴(kuò)增Id1的編碼序列并定向克隆入誘餌質(zhì)粒pHybLex/Zeo国内a片在线视频，構(gòu)建重組誘餌質(zhì)粒pHybLex/Zeo-Id1，酶切鑒定后轉(zhuǎn)化入酵母株EGY48/pSH18-34费一级无码婬片A片AAA毛片，檢測(cè)重組誘餌質(zhì)粒有無非特異激活報(bào)告基因Leu2亚洲美女操逼视频一区二区、LacZ作用；擴(kuò)增并提取成人肺組織文庫(kù)質(zhì)粒国产网站视频，將文庫(kù)質(zhì)粒及重組誘餌質(zhì)粒轉(zhuǎn)化入酵母細(xì)胞国产黄色网站马上看，依次篩選Leu2^＋，Leu2^＋LacZ^＋克卵侵蕹墒臁99成年小黄片；設(shè)置陰性及陽(yáng)性對(duì)照，重復(fù)篩選Leu2＋LacZ＋克隆并排除假陽(yáng)性克隆国产一级A片免费网在线观看，獲取真陽(yáng)性文庫(kù)質(zhì)粒性交黄片国产，進(jìn)行測(cè)序和同源性比對(duì)。

Surprisingly a high degree of nucleotide identity was found with Staph.

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The sequence analysis result indicated that ompH is of hign homology between different serotypes.

序列分析結(jié)果表明，ompH基因在不同血清型之間具有較高的同源性女生精黄色网站。

The deduced maize amino acids share high sequence homology with cyMDH from other species, such as O. sative, M. erystallinum, and G.

序列聯(lián)配與樹狀分析結(jié)果表明色色色色色色色色色播播，該玉米cyMDH序列與多個(gè)物種的cyMDH基因具有高度的同源性。

This gene, together with a phytase gene ( 168phyA) identified in the B. subtilis strain 168 genome by a homology search, was cloned and over-expressed in B. subtilis using a phi105MU331 prophage vector system. Up to 35 units of phytase/ml were secreted into the culture media; and mature enzymes of around 44-47 kDa were purified for characterization.

借助于ф105MU331前噬菌體載體系統(tǒng)免费人成网站视频在线观看，這個(gè)基因和另一個(gè)通過同源性研究并在B.subtilis菌株168的基因組中被鑒定的植酸酶基因(168phyA)激情性爱国内自拍，于B.subtilis中得到克隆和過度表達(dá)。B.subtilis菌體向培養(yǎng)基中分泌了高達(dá)35個(gè)單位的酶夜免费av黄色录像免费的黄色录像，同時(shí)分子量在44-47kDa之間的成熟酶得到純化视频一区二区男人的天堂国产大片免费。

This thesis reviewed the historical development, the current taxonomic status and the phylogeny of rhizobia, and compared the main methods applied in rhizobial polyphasic taxonomy.

本論文綜述了根瘤菌的分類現(xiàn)狀、系統(tǒng)發(fā)育地位及有關(guān)的研究方法自拍偷拍亚洲精品；采用數(shù)值分類一级aa片视频、16S rDNA PCR-RFLP、BOX-PCR国产日韩欧美主播、AFLP亚洲欧美国产色图、16S rDNA 序列分析、G+Cmol%測(cè)定99久久播放视频、DNA-DNA同源性分析等多相分類技術(shù)對(duì)分離自我國(guó)不同地區(qū)的木本豆科植物刺槐日韩三级中文字幕、黃檀亚洲欧洲性爱、合歡根瘤菌進(jìn)行了系統(tǒng)地分類學(xué)上的研究。

Study on systematic taxonomy of isolated halophiles had been done using polyphasic technology including morphological and cultural characteristic obserbations, physiological and chemical tests, analyses of chemtaxonomy and G+C content, DNA-DNA homology and 16S rRNA sequences.

并對(duì)分離到的嗜鹽菌菌株采用形態(tài)特征国产精品久久久久精品三级一区、培養(yǎng)特征觀察在线看一级片视频软件，生理生化測(cè)定，細(xì)胞化學(xué)組分分析www熟女，DNAmol％和DNA同源性測(cè)定丝袜美女人人摸人人操，以及16S rDNA全序列分析等多項(xiàng)分類技術(shù)對(duì)其進(jìn)行系統(tǒng)的分類研究。

Cv. Jingdong No. 1 plumules at thekey stage of 21 d vernalization being recovered as cDNAs after subtraction with mRNAs...

Northem雜交及同源性分析表明它是一個(gè)在植物中新發(fā)現(xiàn)的不同于低溫脅迫誘導(dǎo)基因的春化特異克隆免费一级无码婬片AA片高潮喷水，其對(duì)春化需求型植物的成花誘導(dǎo)可能起重要的調(diào)控作用免费观看黄色视频。

New laccase gene and its full-length cDNA were clone from genomic library and equalized cDNA library of Ganoderma lucidum. Similarity between the sequence and other laccase gene were analyszed with NCBI blast, the results show that the new gene belong to multicopper oxidases gene family and have 60-70% Similarity with other laccase gene.

從靈芝的基因組文庫(kù)和cDNA均一化文庫(kù)中克隆了靈芝的漆酶基因和全長(zhǎng)cDNA，并通過NCBI Conserved Domain Search氨基酸序列的相似性分析及與其它真菌漆酶之間的相似性分析免费在线看欧美三级片，證明得到的蛋白是一種新的multicopper oxidases偷拍偷窥网，其蛋白質(zhì)序列與其它已克隆漆酶基因的同源性在6 0～7 0％之間，并對(duì)論文克隆的漆酶基因的啟動(dòng)子和終止子進(jìn)行了分析欧美国产日韩激情视频。