The nucleotide and amino acid homology of the 5 isolates is higher than 94% and 96% respectively.

PRSV 5個分離物核苷酸序列的同源性在94%以上熟女一级毛片，氨基酸序列的同源性在96%以上。

The novel primers were evaluated by detecting BTV serotypes 1, 3, 5, 8, 10, 11, 21 and 22. The specificity of the primers was estimated by comparing to gene sequences of viruses published in GenBank, and further assessed by detecting BTV serotype 1-12 and Epizootic hemorrhagic disease virus serotype 1-4. The sensitivity and repeatability of PCR with the novel primers were evaluated by successfully detecting the recombinant plasmid pGEM-T121 containing the diagnosed nucleotide sequence.

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For example, we have conducted the sequence screening on the binding sites of the interface of Barnase/Barstar complex and found that our DGA method can not only determine which position is more conservative and more important, but also redesign the binding sites rationally. The random sequence design for α,β,α/β and α+β four folding motifs have also been practiced and revealed that there are both homology evolution and heterology evolution of protein sequences compatible the same backbone motif.

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In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

為了獲取這一基因片段與國外分離株進行同源性比較99reshipin，同時也為了了解MDPV強、弱毒株VP2基因之間的異同關(guān)系99精品视频免费热播在线观看网站，及其免疫原性的變化規(guī)律欧美激情偷乱人伦小说，通過DNA重組技術(shù)，設(shè)計了一對引物L(fēng)HMP7／LHMP8久久播放AV，該對引物選取位于2885～2900及4618～4604的兩段序列日韩毛片一区二区三区，跨幅為1734bp，并在這兩條引物中分別加入兩種限制性核酸內(nèi)切酶SacⅡ和KpnⅠ的酶切位點欧美三级性爱在线，分別對MDPV-Q和由該株病毒經(jīng)人工致弱后得到的MDPV-26株進行PCR擴增免费黄色在线网站，并將PCR產(chǎn)物克隆到pMD18-T載體上，分別得到二個重組子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2黄片·COM。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

為了研制副結(jié)核病敏感九九色色免费视频、特異的診斷試劑和新型、高效的預(yù)防制劑十八岁美女一级片，尤其是DNA疫苗美女插插插视频，本研究篩選了M.paratuberculosis主要保護性抗原SOD基因18岁A级黄色和特级黄色视频，以M.paratuberculosis C-2染色體DNA為模板，以SOD基因的特異性引物進行PCR擴增2022最新韩国三级理论，獲得了624bp的SOD基因九九在线精品国产麻豆，通過T-A克隆技術(shù)，將PCR產(chǎn)物克隆至pMD18-T Vector中欧美在线视频精品加勒比，以質(zhì)粒大小日韩欧美爱爱视频、酶切分析、PCR擴增及序列分析鑒定重組克隆欧美人成在线视频免费一级A片，成功地構(gòu)建出克隆質(zhì)粒pMD18-T-SOD200毛片，序列測定及DNASTAR分析表明，所獲得的M.paratuberculosis C-2 SOD基因與Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致www.色色99，兩者核苷酸序列的同源性為99％色色色色鬼，氨基酸序列的同源性為99.5％，表明該基因在副結(jié)核分枝桿菌中是高度保守的国产一级A片婬片免费。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

為了研發(fā)預(yù)防副結(jié)核病的新型疫苗尤其是DNA疫苗及相關(guān)蛋白功能午夜免费A片，本研究選擇了MAP的主要保護性抗原Hsp65蛋白，以副結(jié)核分枝桿菌C-2株染色體DNA為模板亚洲免费aa视频，以hsp65基因的特異性引物進行PCR擴增黄色视频无遮挡无内衣，獲得了1 626bp的hsp65基因，通過T-A克隆技術(shù)日韩一级片网站，將PCR產(chǎn)物克隆至pGEM-T Vector中欧美性爱在线影视福利网，以質(zhì)粒大小、酶切分析首页一黄色视频、PCR擴增及序列分析鑒定重組克隆国产一级牲交视频播放在线观看，成功地構(gòu)建出克隆質(zhì)粒pGEM-T-hsp65，以DNASTAR軟件分析了所克隆基因的核苷酸序列和推導(dǎo)的氨基酸序列黄色18网站，結(jié)果表明美国一级黄片人兽，所獲得的hsp65基因與GenBank中MAPK-10株該基因核苷酸大小完全一致，兩者核苷酸序列的同源性為99.1％老女人喷水毛片，氨基酸序列的同源性為99.3％www.欧美a片，表明該基因在副結(jié)核分枝桿菌中高度保守。

The predicated gene products of amrA, amrB showed high similarities with the typical response regulator, such as afsQ1, mtrB and ompR, about 40%-62%.

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Pneumoniae FH strain was cloned and the sequence was analysed by M13 DNA sequencing method. Comparing the PCR product sequcence with MP M-129 strain P1 gene, we found that there are 4 bases different. This may result from the different MP DNA templates. The maximum homology is 98.8%. The result confirmed the fidelity and specificity of the amplified target DNA segment by PCP, and suggested that two categories of MP P1 gene still exist a few differences even in the conservation region. The cloning MP DNA segment was labelled by random hexanucleotide priming, after hybridization, the probe detection was completed using an anti-digoxigenin antibody alkaline phosphatase conjugate, and the substrates 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium. This hybridization system is much superior to the radioactive probe hybridization, because it is safe, easy to handle and has no limitation of decay time. The time required for colormetic detection is also much less than the corresponding autoradiographic exposure time needed to achieve similar detection limits with 32P-labelled probes. The Dig-probes could be used repeatedly, and this made them not only much convenient to use, but also lower the cost, and worthwhile to be used popularly.

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Rapid amplification of cDNA end:Retroverse transcription product of total RNA extracted from normal porcine tissue was used as the template,gene specific primers were designed and advantage 2 polymerase mix was used in PCR,of which using porcine genomic DNA as the template:forward primer was designed according to the acquired consensus region of human and pig FGL2 3′ sequences while reverse primer was designed from human FGL2 3′ end downstream sequence;TA cloning.

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Anguillarum isolates, while 94.3% and 91.9% nucleotide identical to L. pelagius and Photobacterium damselae respectively. MP analysis showed that ayu-H080701 shared 97.6%-98.8 % amino acid sequence identical to L. anguillarum isolates, while lower than 75.6 % to other bacteria. Phylogenetic analysis showed that ayu-H080701 grouped constantly with L. anguillarum isolates. The biochemical, physiological tests and sequence analysis all strongly supported the identification of the pathogen causing ayu vibriosis in Ninghai country, China, as an isolate of L.

PCR擴增檢測表明，細菌16S rRNA 基因通用引物和鰻利斯頓氏菌MP基因特異引物均能擴增到預(yù)期大小的特異性條帶欧美巨大XXXX做受高清。ayu-H080701與鰻利斯頓氏菌16S rRNA基因核苷酸序列同源性最高，為99.4%～99.5%尤物网站在线免费看，與同屬的汗⑴木芬磺?1；【兔廊唆~發(fā)光桿菌分別為94.3%和91.9%；ayu-H080701與鰻利斯頓氏菌MP氨基酸序列同源性高達97.6%～98.8 %免费黄色性爱网站，與其它弧菌科成員則低于75.6 %国产交换配乱婬视频偷大叼，系統(tǒng)進化樹分析也揭示ayu-H080701與鰻利斯頓氏菌進化相關(guān)性最高。