The complete nucleotide of F12 shared the highest sequence identity (90.1%) with AYVVTW-[TW:Tai:99] (AF307861), an isolate of Ageratum yellow vein virus from Taiwan. The DNAβmolecule associated with the F12 was found with primersβ01/02 (F12β).

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They were members of MADS box gene family, and which highly homologous to AGAMOUS from Arabidopsis and other known orthologues.

參照單子葉植物的AG類基因序列設(shè)計簡并引物色系综合网，利用RT-PCR的方法人人摸人人干人人操人人舔，從百合中擴增AG到同源片段，并根據(jù)擴增出的片段序列設(shè)計特異引物進(jìn)行5'RACE和3'RACE的擴增性交配网站，最終獲得全長另类A片。

Sensitivity comparison between the novel one-step RT-PCR and DAS-ELISA for PVX revealed that the former method was 20 times more sensitive than the later one. The novel one-step RT-PCR system was also successfully tested with field samples of turnip mosaic virus in 8 different crucifer vegetable crops.

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The origin analysis on the source of hosts and terrains was carried out on the base of homology tree and phylogenetic tree founded in 19 isolates on their CP gene nucleotide sequences. The result showed that the gene types and clusters of Cucumber green mottle mosaic virus were not obviously related to the two factors.

對包括該分離物在內(nèi)的19個CP基因核苷酸序列進(jìn)行同源性比較和系統(tǒng)進(jìn)化樹構(gòu)建，并結(jié)合寄主來源及地域來源進(jìn)行LHP的起源分析超碰在线免费视，結(jié)果表明黃瓜綠斑駁花葉病毒不同分離物的序列分群和進(jìn)化關(guān)系與上述兩個因素?zé)o明顯關(guān)系国产黄片免费在线播放。

To establish a method to differentiate Muscovy duck parvovirus from goose parvovirus, primers GPVF/R1 and MDPVF1/R1 were designed according to the genomic sequences of GPV and MDPV to amplify the virus culture and nucleotide of GPV, MDPV, duck plague virus, duck hepatitis virus, duck reovirus, canine parvovirus and feline panleucopenia virus.

根據(jù)番鴨細(xì)小病毒和鵝細(xì)小病毒基因組的非同源序列各設(shè)計了1對引物MDPVF1/R1和GPVF1/R1，建立了一種PCR方法型男操干美女破处视频网站，用該PCR方法分別對GPV欧美性爱亚洲日韩、MDPV午夜激情毛片在线播放、鴨瘟病毒、鴨肝炎病毒三级日本在线看、鴨呼腸孤病毒日韩最新在线观看网址、犬細(xì)小病毒和貓泛白細(xì)胞減少癥病毒的病毒培養(yǎng)物及其核酸進(jìn)行擴增。

In order to direct the construction of plant germplasms by elucidating the relatives among plants at the level of gene, CYP86MF gene analogues from 11 species of 6 genera in Cuciferae were respectively obtained by PCR strategy using gene specific primers designed from conserved regions of CYP86MF gene reported. Sequence comparisonindicated that the similarities among the genes at nucleotide level were over 80%, and the similarities at amino acid level remained above 70%. The differences between the genes at nucleotide and amino acid level between species were 1.0%~ 5.7% and 2.6%~ 7.3% respectively, while those between genera 5.6%~ 22.5% and 7.3%~ 31.2%, respectively. Phylogenetic analysis showed that Brassica was closely related to Raphanus, followed by Rorippa Scop, Arabidopsis Heynh, Capsella Medic orderly, most distantly related to Orychophrogmus.

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The genetic DNA was extracted from the blood of 6 A. forsteri which sex was unknown. The PCR amplified fragments with primers P2/P8 were cloned in T-Vector. Taking homologous sequence of Circaetus gallicus as reference, the CHD gene sequence was compared to the homologues in GenBank to identify its sex.

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DNA sequence which was partial homogeneous with Copper-transporting P-type ATPase gene was amplified from filaceous fungi Penicillium janthinillum GXCR by designed degenerate primers based on the reported amino acid sequence.

根據(jù)已報道的與銅轉(zhuǎn)運P型ATP酶同源的氨基酸序列久久三区，設(shè)計簡并引物，在絲狀真菌Penicillium janthinillum GXCR中擴增得到與銅轉(zhuǎn)運P型ATP酶基因同源的部分序列在线激情A片。

A chimeric primer was designated in which segment A near 3' end is complementary to HB plus strand just before the DR2 region gap, and segment B near 5' end is consensus to part of the human immunodefficient irus genomic sequence, without homogenetic relationship to HB genome.

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It was reported an cDNA encoding HMG protein was cloned from Pharbitis nil.

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