Polymerase chain reaction amplification of a portion of a gene encoding a surface protein of Wolbachia was used to detect the Wolbachia infection of the population of Pteromalus puparum and Nasonia vitripennis by universal primers of wsp gene and the specific primers for wsp genes of A and B Wolbachia supergroup.

采用Wolbachia 的通用引物及A和B大組特異性引物對蝶蛹金小蜂Pteromalus puparum和麗蠅蛹集金小蜂Nasonia vitripennis體內(nèi)Wolbachia的wsp基因進(jìn)行PCR擴(kuò)增及序列測定人人妻人人艹，并對測定的序列進(jìn)行了同源性比較和基因特征分析日韩欧美黄片在线一区。

To study the molecular structure and function of the PyNPase, and to construct a system for preparation of the enzyme for practical synthesis of nucleoside analogs, molecular cloning of the encoding the PyNPase from E. aerogenes strain EAM-Z〓 have now been completed.

我們通過以該酶的N端氨基酸序列設(shè)計(jì)上游引物，以不同來源PyNPase的C末端同源區(qū)氨基酸序列設(shè)計(jì)下游引物人人人摸摸人人人操，采用PCR技術(shù)日韩高清AV在线电影网，擴(kuò)增出該酶基因的編碼區(qū)（約1206bp），并完成了其重組質(zhì)粒的構(gòu)建和核苷酸序列的測定AV在线播放八区，目前正在進(jìn)行PyNPase表達(dá)質(zhì)粒的構(gòu)建亚洲女妇在线观看视频。

The total RNA was abstracted from the larvae of Boophilus microplus, and a 1982bp Bm86 gene was amplified by RT-PCR. The target gene was subcloned into T vector. The sequencing showed that the nucleotide sequence of the cloned Bm86 gene shared 97.4% homology with the data published in and this fragment contained the complete open reading frame of Bm86 gene.

為了克隆微小牛蜱Bm86 基因及構(gòu)建該基因的表達(dá)載體，以微小牛蜱饑餓幼蜱的破解物提取的總RNA為模板黄页网址大全在线播放免费观看，參照已發(fā)表的微小牛蜱Bm86基因的核苷酸序列www...一级国产，設(shè)計(jì)了1對引物，采用RT-PCR技術(shù)獲得微小牛蜱Bm86基因欧美日韩·A级黄片视频1；將Bm86基因克隆入載體www.妓女网，并進(jìn)行序列分析，結(jié)果證明在线观看亚洲人成网站A片，克隆的Bm86基因序列與GenBank上登錄的Bm86基因序列的同源性達(dá)97.4%欧美AA级黄片，而且該序列包含完整的開放閱讀框。

It is not known how rare this resemblance is, or whether it is most often seen in inclusions of silicates such as garnet, whose crystallography is generally somewhat similar to that of diamond; but when present, the resemblance is regarded as compelling evidence that the diamonds and inclusions are truly congenetic.

現(xiàn)在尚不知這種類似稀少到何種地步国产黄片WW，也不知道是否它最常見于象石榴石一類的硅酸鹽內(nèi)含物中国产永久在线更新，而這類物質(zhì)的晶體結(jié)構(gòu)普遍地在某種程度上類似于金剛石的晶體結(jié)構(gòu)。但一旦存在人人看人人爽，這種類似就被視作極有說服力的證據(jù)www.91精品.com，證明金剛石與內(nèi)含物確是同源的。

It is not known how rare this resemblance is, or whether it is most often seen in inclusions of silicates such as garnet, whose crystallo graphy is generally somewhat similar to that of diamond; but when present, the resemblance is regarded as compelling evidence that the d iamonds and inclusions are truly congenetic.

現(xiàn)在尚不知這種類似稀少到何種地步，也不知道是否它最常見于象石榴石一類的硅酸鹽內(nèi)含物中伦理天堂百度影音在线观看网站，而這類物質(zhì)的晶體結(jié)構(gòu)普遍地在某種程度上類似于金剛石的晶體結(jié)構(gòu)囯产深喉视频一区二区。但一旦存在，這種類似就被視作極有說服力的證據(jù)黄页免费视频大全，證明金剛石與內(nèi)含物確是同源的香港一级A毛片在线播放。

The multi-drug resistance rate of Salmonella and E.coli were 91.80% and 100%.According to the genes sequence published in Genbank ,we analysed the value of Tm ,content of G+C, oligonucleotide dimer by DNAStar and Primer 5.0 software, and according to the construction of I integron ,we designed 3 pairs of primers to amplify intIl gene ,variable segment ,3"-conserved segment for detectting Salmonella and E.coli"s I integron.As a result , aimed genes were amplified and sequenced;The detection rate of I integron of 61 Salmonella and 48 E.coli were 81.97% and 66.67%.

據(jù)文獻(xiàn)報(bào)道及GenBank中注冊的基因序列，運(yùn)用DNAStar軟件分析同源率及Primer5.0軟件對Tm值99精品A片一级视频、G+C含量中国一级黄片播放、引物二聚體等進(jìn)行分析，根據(jù)Ⅰ類整合子的結(jié)構(gòu)設(shè)計(jì)了3對引物日韩黄色网站www...，對Ⅰ類整合子的intI199久久久国产一区二区三区、可變區(qū)、3'保守端進(jìn)行擴(kuò)增91麻豆精品国产福利在线，檢測沙門氏菌和大腸桿菌Ⅰ類整合子美女张开腿黄网站免费直播；擴(kuò)增出了相應(yīng)基因片段并測序；61株沙門氏菌和48株大腸桿菌中分別有50株和32株菌檢測出Ⅰ類整合子A片一区，Ⅰ類整合子的檢測率分別為：81.97％和66.67％日本国产一级。

According to one conserved sequences of phage GIL01 from Bacillus thuringiensis,a pair of primers was designed.With overnight cultivations of 35 B.t standard strains and one fermentative strain MZ1 as templates,PCR results showed that 16 strains,which may be lysogenic strains,produced the product with the size about 750 bp as anticipated.

根據(jù)蘇云金桿菌(Bacillus thuringiensis， B.t)溶原性噬菌體GIL01(GenBank Accession Number： AJ536703)的同源序列設(shè)計(jì)一對引物爆乳无码一区二区在线观看AI，用35株B.t標(biāo)準(zhǔn)菌株和生產(chǎn)菌株MZ1(血清型H3a3b)的過夜培養(yǎng)物作模板欧美三级黄色，結(jié)果有16株菌株包括MZ1能擴(kuò)增出大小約為750 bp的預(yù)期產(chǎn)物，推測這16株菌為溶原菌你懂的免费视频在线观看。

Among them strain MZ1 had been confirmed a lysogenic strain by indicator strain ZK1 after producing phage plaques induced by Mitomycin C.Two templates of overnight cultivation and its DNA from strain MZ1 were then amplified in the same time with five pairs of primers designed from the same phage,and the same products were obtained,suggesting that strain MZ1 was also confirmed a lysogenic strain by PCR method.

MZ1菌株的過夜培養(yǎng)液與指示菌ZK1(血清型H25)混勻并在雙層瓊脂上培養(yǎng)12 h后出現(xiàn)了滴度為2×107 pfu的噬菌斑亚洲最大A片，從而證明生產(chǎn)菌株MZ1確為溶原菌；又以GIL01同源性較高的其它四個基因設(shè)計(jì)引物国产日本黄色付费网站，分別以MZ1的培養(yǎng)物及其溶原性噬菌體基因組為模板進(jìn)行PCR擴(kuò)增www.一级片在线观看，結(jié)果兩者得到幾乎一致的帶型，進(jìn)一步證明了生產(chǎn)菌株MZ1為溶原菌999超碰，說明利用PCR法檢測鑒定蘇云金桿菌的溶原性是可行的污污污在线观看黄真人网站。

The nucleotide sequences of the PAP gene and PAPⅡ gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPⅡ gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99. 8% were observed respectively. They both had one open reading frame . Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陸根為材料，提取其總RNA欧美VA影院、反轉(zhuǎn)錄国产超碰精品热久久91，用一對PAP基因的特異性引物和一對PAPⅡ的特異性引物，分別對反轉(zhuǎn)錄產(chǎn)物進(jìn)行PCR擴(kuò)增全免费的黄色网站，將擴(kuò)增的目的片段進(jìn)行序列分析九九视频在观看视，結(jié)果擴(kuò)增出來的PAP基因與已經(jīng)報(bào)道的序列完全相同，擴(kuò)增出來的PAPⅡ基因與已經(jīng)報(bào)道的序列同源性也在99.8％欧美性爱巨吊，而且它們都各自編碼一個開放閱讀框架激情在线另类小说图片，可以說美洲商陸根中肯定存在PAP和PAPⅡ基因色一色最新地址，并已經(jīng)轉(zhuǎn)錄。

The nucleotide sequences of the PAP gene and PAPII gene in the root of Phytolacca americana were determined by reverse transcription polymerase chain reaction and PCR. The gene comprises 942bp and 933bp respectively, encoding 313 amino acids and 310 amino acids. When PAP gene and PAPII gene sequences in the root were compared with those in the spring leaves and summer ones, the highest similarity 100% and 99.8% were observed respectively. They both had one open reading frame. Analysis of the two sequences showed, in fact, they belonged to one species.

以美洲商陸根為材料免费的又大又粗又爽的视频，提取其總RNA久久精品国产精五月婷、反轉(zhuǎn)錄，用一對PAP基因的特異性引物和一對PAPⅡ的特異性引物1024手机看片欧美日韩你懂的，分別對反轉(zhuǎn)錄產(chǎn)物進(jìn)行PCR擴(kuò)增亚洲欧美日韩一区在线，將擴(kuò)增的目的片段進(jìn)行序列分析，結(jié)果擴(kuò)增出來的PAP基因與已經(jīng)報(bào)道的序列完全相同99re视频在线这里精品，擴(kuò)增出來的PAPⅡ基因與已經(jīng)報(bào)道的序列同源性也在99.8％久久精品国产88久久综合，而且它們都各自編碼一個開放閱讀框架，可以說美洲商陸根中肯定存在PAP和PAPⅡ基因亚洲欧美卡通图区丝袜，并已經(jīng)轉(zhuǎn)錄欧美黄动漫在线观看。