The key research progresses and results that we accessed by the project were as follows: 1 A pair of degenerated primers were designed in the conserved domain which based on the amino acid sequence of HAK in Arabidopsis thaliana. The 500 bp fragment of HAK were amplified from the roots of Puccinellia tenuiflora by RT-PCR. The full sequence of HAK were obtained by 5' and 3' RACE methods and were determined and analyzed.

本項(xiàng)目主要取得了以下研究進(jìn)展和成果：（1）根據(jù)已有HAK轉(zhuǎn)運(yùn)蛋白氨基酸序列在保守區(qū)域設(shè)計(jì)簡(jiǎn)并PCR引物，用反轉(zhuǎn)錄PCR方法网站黄色a，擴(kuò)增出500bp的小花堿茅根HAK片段黄色网站免费观看视频国产，通過(guò)5''和3''RACE的方法獲取了該HAK的全長(zhǎng)序列，完成了測(cè)序和序列同源性分析人妻系列无码专区系列。

Because homeotic gene mutation and floral organ variation is a direct relationship,therefore the mutant application is the current functional genomics study developmentdirection. We discovered one mutant ah from line 458, which was a line ofthe twin-seedling strain W2555.ln this study, we have investigated the morphogenesisprogress, genetic analysis of mutant traits and molecular tagging of related gene.

目前性69式性无遮挡免费视频，由于在水稻中發(fā)現(xiàn)的花器官突變體很少，所以對(duì)水稻花發(fā)育的研究主要是利用雙子葉植物MAS-box基因的保守序列設(shè)計(jì)引物特级欧美婬片免费高直播播放，在水稻基因組或者cDNA文庫(kù)中篩選與雙子葉植物花器官發(fā)育相對(duì)應(yīng)的MADS基因免费的黄色av，因?yàn)橥串愋突虻耐蛔兣c花器官的變異是直接對(duì)應(yīng)的關(guān)系，所以突變體的應(yīng)用是當(dāng)前功能基因組學(xué)的發(fā)展方向欧美黄色成年网站视频。

Methods Granulocyte/macrophage colony stimulating factors and interleukin 4 were used to cultivate DC from peripheral blood of hepatocellular carcinoma patients.Tumor associated antigen extracted from human liver cancer HepG2 cell line was used to activate the DC,which will initiate homogenic T lymphocyte to form cytotoxic T lymphocyte.HepG2 cells were transplanted to the nude mice and were treated with CTL.Expression of PCNA in tumor cells was evaluated.

聯(lián)合應(yīng)用粒/巨細(xì)胞集落刺激因子及白介素-4(IL-4)直接從肝癌患者外周血中培養(yǎng)出DC国产免费看视频黄片；以源于人肝癌細(xì)胞系HepG2細(xì)胞的腫瘤抗原粗提物刺激DC；DC激活同源的T淋巴細(xì)胞產(chǎn)生細(xì)胞毒性T淋巴細(xì)胞麻豆A片在线观看；建立人肝癌細(xì)胞系HepG2裸鼠移植瘤模型国产97av在线播放；CTL治療人肝癌細(xì)胞系HepG2裸鼠移植瘤；檢測(cè)移植瘤標(biāo)本腫瘤細(xì)胞PCNA表達(dá)水平视频一区二区久久91。

These results demonstrated that BmCPV,DsCPV and LdCPV were homologous as classfied in the same electropheres type I,and that this RT\|PCR assay could be used for early,rapid,sensitive and specific detection of DsPCV infection in the natural population of the pine moth,due to the apparent different feeding habits of Bombyx mori,Dendrolimus ssp.and Lymantria dispar larva each other and the no infection of BmCPV and LdCPV to Dendrolimus ssp.

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The Sry gene sequence of Muntiacus crinifrons was acquired and the conclution was that there are 83% homology between the human and Muntiacus crinifrons. It was testified that in all mammal Sry gene is consertive. On the other side the Sry gene was located to the Y2 chromosome of the Muntiacus crinifrons.

最后提取雄性黑麂的基因組DNA，并用同一對(duì)引物對(duì)其進(jìn)行擴(kuò)增午夜视频在线观看高清在线www，亦得到Sry基因的片段最新亚洲亚黄色，對(duì)此擴(kuò)增片段進(jìn)行克隆，測(cè)序欧洲熟妇XXXXX老妇，結(jié)果表明其與Y2染色體得到的Sry基因片段完全一樣五月天六月缴情婷婷婷天天天，與人SRY基因的同源性均為83%。

The genetic identity was determined by the identical numbers and location of bands showed in products of repetitive extragenic palindromic se...

利用重復(fù)片段引物PCR的基因分型方法国际黄色在线视频，根據(jù)條帶數(shù)目和位置完全相同為同一基因型的分型方法[1]判定是否存在基因同源性www.tzbeta.com。

The genetic identity was determined by the identical numbers and location of bands showed in products of repetitive extragenic palindromic sequence-based PCR ..

利用重復(fù)片段引物PCR的基因分型方法，根據(jù)條帶數(shù)目和位置完全相同為同一基因型的分型方法[1]判定是否存在基因同源性久久99精品国产免费。

The results of sequencing was contrasted by BLAST software. The sequence of the PCR products shared 100% homology with Pasteurella multocida reference strain.

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The phylogenic tree of LYSV was clustered into four groups.

LYSV不同分離物間CP氨基酸序列存在明顯差異日韩性色生活免费观看，同源性在75.8%～100%之間欧美一级媱片^片久久精品。

The identification of protein were performed by searching the mass spectrometry data from the NCBInr database using the software of Sequest Bioworks3. 2. The results indicated that over 25 protein components including acid and basis phospholipase A2, nerve growth factor, presynaptic neurotoxin, hemorrhagic factor, thrombin-like enzyme, serine protease, glycogen synthase, metalloproteinase, maturase, L-amino acid oxidase, plasminogen activator, adhesion, cysteine-rich secretory protein, disintegrin, fibronectin-binding protein, fibrinogen clotting inhibitor A chain, vascular apoptosis-inducing protein 1, pyranose 2-oxidase, carbamoyl-phosphate synthase, as well as the homology proteins of snake venom ablomin, ablomin, catrin, mucrofirase, pallabin, salmosin and triflin homologues were identified from the GSSV.

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