In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.

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After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

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The expression of NSE was detected in cortex of adrenal cortical hyperplasia, cortical adenomas and pheochromoeyte carcinoma, hut not in that of pheochromocytoma and normal adrenal. The expression of SYN was detected in cortex of corticohyperplassia, cortical adenomas, medull neoplasm, chromophile tumor and pheochromocyte carcinoma, but not in that of cortical adenocarcinoma and normal adrenal. The expressions of CgA, NSE and SIN were detected in the medulla of all cases. Conclusion The characteristic of CgA, SYN and NSE in cortex and medulla of adrenal are useful in the differential diagnosis of tumor and hyperplasia in the adrenal.

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Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

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Thermoanaerobacter tengcongensis is a rod-shaped, Gram-negative, anaerobic eubacterium that was isolated from a fresh water hot spring in Tengchong, China. Using a"whole-genome-shotgun"method, we sequenced its 2, 689, 445-bp genome from an isolate, MB4〓(Genbank Accession Number AE008691). The genome encodes 2, 588 predicted coding sequences . Among them, 1, 764 (68. 2％) are classified according to homology to other documented proteins, and the rest, 824 CDS (31. 8％), are functionally unknown. One of the interesting features of the T. tengcongensis genome is that 86. 7％ of its genes are encoded on the leading strand of DNA replication.

中文題名騰沖嗜熱厭氧菌全基因組測序及分析副題名外文題名 A complete sequence of the T.tengcongensis genome 論文作者包其郁導(dǎo)師楊煥明研究員學(xué)科專業(yè)遺傳學(xué)研究領(lǐng)域\研究方向?qū)W位級(jí)別博士學(xué)位授予單位中國科學(xué)院遺傳與發(fā)育生物學(xué)研究所學(xué)位授予日期2002 論文頁碼總數(shù)88頁關(guān)鍵詞騰沖嗜熱厭氧菌基因組嗜熱菌館藏號(hào)BSLW /2003 /Q939.1 /33 騰沖嗜熱厭氧菌（MB4＇）是分離于我國騰沖地區(qū)溫泉內(nèi)的革蘭氏陰性厭氧真細(xì)菌。

Neutrophil is an effective phagocyte and is considered to be an important part of innate immunity. An impaired neutrophil microbicidal capacity may increase the susceptibility to infection in stressed athletes.

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A series PCR amplification for differential control strains and DNA samples diluted gradient (1:10) have been used to evaluate the specificity and sensitivity of PCR assay established.Results 1. Detection of GAS by PCR assay: The 345bp specific fragment of speB gene were amplified in all the tested GAS strains including three strains of scarlet fever, whereas it was detected in none of the differential control strains. The lowest limit of detection was 6.5pg genome DNA of GAS strain. 2. Detection of corynebacterium diphtheria by PCR assay: The318bp specific fragment of toxB gene were amplified in all the tested toxigenic corynebacterium diphtheria strains, whereas it was detected in none of the differential control strains. The lowest limit of detection is 850fg/μl genome DNA of corynebacterium diphtheria strain. 3. Detection of Lp by PCR assay: The 340bp specific fragments of mip gene were amplified in all the tested Lp strains, whereas it was detected in none of the differential control strains including three strains of non-pneumophila.

結(jié)果：1、用PCR方法檢測A組鏈球菌：以A組鏈球菌致熱性外毒素基因speB為靶序列青青超碰，設(shè)計(jì)的擴(kuò)增引物對(duì)全部對(duì)照菌株的擴(kuò)增結(jié)果為陰性乱淫网站，而全部A組鏈球菌參考株均能擴(kuò)增出特異的345bp片段，其中包括三株猩紅熱鏈球菌午夜操B，檢測敏感性為6.5pg／μl DNA.2[国产原创中文]国产AV、用PCR方法檢測白喉?xiàng)U菌：以白喉外毒素基因toxB為靶序列，設(shè)計(jì)的擴(kuò)增引物對(duì)全部白喉?xiàng)U菌參考株均能擴(kuò)增出特異的318bp片段黄片毛片在线观看，而全部對(duì)照株的擴(kuò)增結(jié)果為陰性美国乱滛免费视频，檢測敏感性為850fg／μl DNA.3、用PCR方法檢測嗜肺軍團(tuán)菌：以嗜肺軍團(tuán)菌巨噬細(xì)胞感染增強(qiáng)子基因mip為靶基因www18岁，設(shè)計(jì)的引物對(duì)嗜肺軍團(tuán)菌14個(gè)血清型參考株均擴(kuò)增出特異的340bp片段久久超碰男人的天堂，而鑒別對(duì)照株包括三株非嗜肺軍團(tuán)菌均未擴(kuò)增出任何片段。

BE1 monoclonal antibody can be used to detect basophils in tissue.

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The probe of chip includes the entire genomic DNA of at least ten thiobacillus ferrooxidans, at least two thiobacillus acidophilus sulfur oxides, at least one eosinophils thiobacillus, at least one acidithiobacillus albertensis bacteria, at least six thiobacillus oxidation of ferrous - Lo, at least eight heterotrophic bacteria acidophilus, at least one metal leaf sulfur bacteria, at least one Acidianus tengchongensis, at least a Metallosphaera sedula and at least two of thermophilic bacillus curing.

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