Jejune adj,空洞的,不成熟的（devoid of significance or interest:dull

je 諧音：摘。June 六月欧美粗大激情一级视频。聯(lián)想：六月摘的果子加勒比综合网站，當(dāng)然是不成熟的夜夜视频网。

Immature or silly 不成熟的,傻的: She was not a preppy girl as she looked.

她并非如外表看上去的那樣是個(gè)不成熟的女孩。

Immature 尚未成熟的 It is hard for an unfledged writer to find a sympathetic publisher.

初出茅廬的作家要找到投緣的出版商很不容易巨乳在线白虎在线。

The results showed that the rates of mature oocyte were 75.2％, 73.1％, 69.8％, 63.5％and oocyte at telophase of MI were 16.3％, 15.9％, 16.9％, 27.0％, respectively. The rates of maturation of oocytes cultured in TCM199 with serum, EGF and TGFαwere significantly higher than that of with BSA team (P＜0.05) and the number of oocyte stayed at telophase of MI in TCM199 with serum, EGF and TGFαteam were significantly less than that with BSA team(P＜0.05); Significant higher rates (66.6％, 66.6％, 73.6％) of normalα-tubulin distribution in oocytes cultured in TCM199+ serum, EGF and TGFαwere compared to that of TCM199+BSA(43.3％)(P＜0.05). The rates of oocyte with cortical granules in cortex were 58.8％, 33.9％, 54.7％and 47.9％respectively, there was significant difference between oocytes cultured with serum, EGF and BSA(P＜0.05). In conclusion, TGFαand EGF can promote the oocyte nuclear transition from telophaseⅠto metaphase of meiosisⅡ, and improved the expression and distribution ofα-tubulin during the ovine oocytes maturation; EGF and serum could promote oocyte cytoplasm maturation. The results suggested that EGF and TGFαmight substitute some substance in serum to improve the quality of oocyte nucleus maturation in vitro, but EGF might be more functional than TGFαto promote the maturation of ovine oocyte ooplasm.

三黄黄网站点击进入免费、EGF和TGFα對(duì)卵母細(xì)胞成熟的影響未成熟卵母細(xì)胞分別在TCM199基礎(chǔ)培養(yǎng)液+血清、TCM199基礎(chǔ)培養(yǎng)液+BSA+EGF国产高清wwwwwwwww色视频、TCM199基礎(chǔ)培養(yǎng)液+BSA+TGFα和TCM199基礎(chǔ)培養(yǎng)液+BSA四組成熟培養(yǎng)系統(tǒng)中成熟培養(yǎng)黄网站色视频网站免费观看，22小時(shí)后上述各組卵母細(xì)胞的核成熟率分別為75.2％、73.1％洲在线综合视频、69.8％欧美老年人性爱视频、63.5％，處于第一次減數(shù)分裂末期的比率分別為16.3％男女啪啪激烈高潮网站、15.9％久久AA视频、16.9％、27.0％午夜欧美黄色，在添加血清欧美日本高清、EGF、TGFα各組中核成熟率顯著高于其它各組(P＜0.05)黄色网站直接看的，處于末期的比例明顯低于其它各組(P＜0.05)：添加血清草草欧美视频在线播放二区、EGF、TGFα各組在a-Tubulin蛋白的分布與表達(dá)上(66.6％91视频三级片、66.6％黄粗暴高潮小说视频、73.6％)明顯高于BSA組的正常率43.3％(P＜0.05)；各組CG發(fā)生遷移較好的卵母細(xì)胞的比率分別為58.8％在线www.aaa、33.9％欧美性爱俺去操你啦、54.7％、47.9％国产免费一区二区视频，血清組和添加EGF組CG遷移至皮質(zhì)區(qū)的比例明顯高于僅添加BSA處理組(P＜0.05)中文字幕又黄又刺激的的网站。

Maturity of Lower Cretaceous source rock, researched by the authors, is closely relative to the deposition thickness of the Tertiary and the Quaternary. It shows that source rock at the depth of 4,000-4,400m is low mature, and 4,400-5,200m is in the peak of oil generating. Correspondingly, the source rock of the Zhonggou Formation is immature; the Upper Xiagou Formation is low mature; and the Lower Xiagou Formation and the Upper Chijinpu Formation are in the peak of oil-generating; and the Lower Chijinpu Formation is at the stage of generation of condensate-wet gas with high maturity. There is no source rock reaching the overmature stage of dry gas at present.

可溶有機(jī)質(zhì)轉(zhuǎn)化率、鏡質(zhì)體反射率真实处破在线播放、熱解午夜视频在线国产郎色、生物標(biāo)志物特征等參數(shù)表明：青南凹陷中部的下白堊統(tǒng)烴源巖生油門(mén)限深度為4000m（相應(yīng)的鏡質(zhì)體反射率為0.65％）超碰caop0rn超碰分类草棚，埋深4000～4400m為低成熟階段，埋深4400～5200m為成熟生油高峰階段啊片免费看，因此中溝組烴源巖未成熟欧美三级片视频免费，下溝組上部烴源巖低成熟激情久久综合精品久久久久，下溝組下部和赤金堡組上部烴源巖處于成熟生油高峰階段日韩精品芒果视频下载，赤金堡組下部烴源巖處于高成熟的凝析油—濕氣階段，目前尚無(wú)烴源巖達(dá)過(guò)成熟干氣階段亚洲日韩高清AⅤ在线观看。

To pronuclear formation rate after oocyte in vitro maturation 18 hours and parthenogenticactivation, oocytes derived from SM is significantly higher than oocytes from PM,oocytes derived from IM is significantly lower that of oocytes detrived from PM and SM.

對(duì)于孤雌刺激反應(yīng)而言人人操人人干47，無(wú)論是何種類(lèi)型的卵母細(xì)胞成熟18小時(shí)后，自發(fā)成熟卵母細(xì)胞激活后形成原核的比率高于被動(dòng)成熟的卵母細(xì)胞黄色一级片一级片，誘導(dǎo)成熟的卵母細(xì)胞的原核形成率顯著低于自發(fā)和／或被動(dòng)成熟的卵母細(xì)胞www,bbb国产。

Our study includes four aspects. In the first aspect we study several important conditions of porcine oocytes maturation in vitro and oocytes cleavage after parthenogenetic activation and found mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15% PFF+0.57mMcysteine is a good culture condition .When the Cocs are cultured in it ,the maturation rate and oocytes cleavage rate are higher than those of foreign covered. Our result are (86.7±3.35)% and (86.3±4.16)% and the highest report of foreign is(85.7±4.1)%.In the second aspect we study the effect of different chemical activations on development of porcine parthennogenetic embryo and found two best activation method. The first one is that putting the maturation MII oocytes in the 20μmol/L ionomycin for 30 minutes and then putting them in the NCSU-23 condition containing 5μg/mICB and 5mM/L6-DMAP for 3.5 hours, the oocytes cleavage rate and morulae/blastocysts development rate are (76.7±7.6)% and (37.1±6.4)%.The second one is that putting the maturation MII oocytes in the 200μM/L Thimerosal for 20 minutes and then putting them in the NCSU-23 condition containing 8mM DTT for 30 minutes

本研究分為4個(gè)部分，第一部分對(duì)影響豬卵母細(xì)胞體外成熟和孤雌激活后胚胎分裂的幾個(gè)重要條件進(jìn)行了比較研究男人女人插动态，確立了一種較好的培養(yǎng)方法：與顆粒細(xì)胞共培養(yǎng)亚洲国产欧美日韩A片在线观看，找到了一種適合豬卵母細(xì)胞體外成熟的培養(yǎng)基：mNCSU-23+15IU/mlPMSG+20IU/mlHCG+15%PFF+0.57mM半胱氨酸，成熟率和分裂率分別為(86.7±3.35)%和(86.3±4.16)%亚洲国产欧美日韩AⅤ，國(guó)外報(bào)道的最高成熟率為(85.7±4.1)%2021无码影片；第二部分對(duì)豬卵母細(xì)胞孤雌激活的化學(xué)方法進(jìn)行了研究，確立了化學(xué)激活豬卵母細(xì)胞的兩種最佳方法：1將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞用20μmol/Lionomycin作用30min九九视频精品38在线播放，再將卵母細(xì)胞培養(yǎng)于含5μg/mlCB和5mM/L 6-DMAP(6-二甲基氨基嘌呤)的NCSU-23培養(yǎng)液中国产第一区精品，卵裂率和桑囊胚發(fā)育率達(dá)到(76.7±7.6)%和(37.1±6.4)%2將成熟的去卵丘顆粒細(xì)胞的MII期卵母細(xì)胞在200μM/L的Thimerosal中處理20min，再與8mM的DTT共孵育30min欧美乱婬视频婬色，卵裂率和桑/囊胚形成率為(81.0±2.8)%和(39.6±2.7)%人人摸人人操人人看人人干；第三部分對(duì)孤雌激活胚胎的培養(yǎng)條件進(jìn)行了研究，確立了一種最佳的胚胎培養(yǎng)條件：在SOF簡(jiǎn)單培養(yǎng)基中添加顆粒細(xì)胞進(jìn)行前3天的培養(yǎng)日韩新片免费无码观看视频网站下载，然后轉(zhuǎn)入添加胎牛血清的NCSU—23培養(yǎng)基并和輸卵管上皮細(xì)胞進(jìn)行后期的培養(yǎng)日韩精品欧美国产精品亚，其桑椹胚和囊胚的發(fā)育率為(59.5±3.2)%；第四部分研究了IGF-I

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

結(jié)果顯示：(1)在成熟液中添加蛋白質(zhì)可以加強(qiáng)卵丘細(xì)胞的擴(kuò)散最刺激得黄色网站，但豬卵母細(xì)胞的核成熟與其周?chē)穆亚鸺?xì)胞擴(kuò)展沒(méi)有必然的聯(lián)系制服丝袜中文字幕精品，卵丘細(xì)胞擴(kuò)散或成放射狀不宜作為豬卵母細(xì)胞核成熟的標(biāo)準(zhǔn)，只有排出第一極體才能作為豬卵母細(xì)胞核成熟的標(biāo)志国产免费A；(2)在豬COCs的46-48小時(shí)成熟培養(yǎng)的后23-24小時(shí)階段去除成熟液中的激素不但可以保證卵母細(xì)胞的核成熟率操逼操，而且可加強(qiáng)卵丘細(xì)胞的擴(kuò)散；(3)在現(xiàn)有實(shí)驗(yàn)條件下日本WWWⅹⅹX，在mTCM和mNCSU中添加10％pFF與在mNCSU中添加10％FCS相比可獲得較高的豬卵母細(xì)胞核成熟率美韩日色图；(4)在不同的成熟液中添加不同的蛋白質(zhì)對(duì)豬卵母細(xì)胞核成熟率的影響效果不一樣，但對(duì)體外受精后的早期胚胎發(fā)育影響不明顯欧美一级一片在线观看；(5)成熟液種類(lèi)對(duì)豬卵母細(xì)胞的卵丘細(xì)胞擴(kuò)散和體外受精后的早期胚胎發(fā)育無(wú)顯著影響色哟哟在线看。

The word 成熟 in lay man term means well-cooked, instead of mature

記者：你覺(jué)得自己是幾成熟的藝人伊人精品视频中文字幕？

Japonicus.Nerve extraction substance of sea star and dithiothreitol has been reported to be effectively to induce oocyte maturation in sea cucumbers. In this study we tested their effects on oocyte maturation induction in sea cucumber A. japonicus. The results are as follows, oocyte of A. japonicus does not get mature spontaneously. Neither NES alone nor NES with follicle suspension can induce oocyte maturation. While DTT can prominently increase the percentages of germinal vesicle break down. When the concentration of DTT is between10-1 mol/L and 10-3 mol/L, the effect of maturation induction is more sufficient. When the concentration is about 10-2 mol/L, the percentage of GVBD can reach up to more than 90%.

分別利用在海參中有誘導(dǎo)作用的海星神經(jīng)提取液和二硫蘇糖醇對(duì)從成熟卵巢解剖出的刺參卵母細(xì)胞進(jìn)行誘導(dǎo)成熟試驗(yàn)，結(jié)果顯示≡级毛片，刺參的卵母細(xì)胞在海水中不會(huì)發(fā)生自發(fā)成熟啊啊啊操死你91视频，海星神經(jīng)提取液以及神經(jīng)提取液加濾泡懸浮液對(duì)卵母細(xì)胞沒(méi)有誘導(dǎo)成熟的作用，而二硫蘇糖醇處理可以誘導(dǎo)卵母細(xì)胞生發(fā)泡破裂亚洲毛片在线看，當(dāng)DTT溶液的濃度處于10-1 mol/L到10-3 mol/L之間時(shí)国产精品性爱小视频，對(duì)刺參卵母細(xì)胞的促熟作用較為明顯，在10-2 mol/L左右時(shí)国产日韩欧美一区二区在线播放，卵母細(xì)胞的誘導(dǎo)成熟率可以達(dá)到90%以上国产丝袜专区在线观看。