Oogonium develops into early oocyte in the ovary, and then the oocyte leaves the ovary for the coelomic fluid in the form of single cell or cell mass followed by the rapid separation of the group of oocytes into individual ones. Oocyte enters into the nephridium after its maturation. The rupture of germinal vesicle marks the oocyte maturation. Oocyte in the coelom does not have follicle membrane and vitelline membrane is formed and developed by the oocyte itself. Smaller oocyte (0μm in diameter) is round, and larger ones (≥60μm in diameter) is ovate. The short and long diameters of a morphologically mature oocyte are about 115—120μm and 140—145μm respectively, and the vitelline membrane is 7—9μm thick.

卵原細(xì)胞在卵巢中發(fā)育至早期卵母細(xì)胞時(shí)期單個(gè)或成團(tuán)脫離卵巢入體腔液中人人草操，卵母細(xì)胞團(tuán)細(xì)胞很快分離為單個(gè)細(xì)胞色综合欧美色综合七久久；卵母細(xì)胞在體腔液中發(fā)育成熟后進(jìn)入腎管欧美操女视频；生發(fā)泡破裂是卵母細(xì)胞成熟的標(biāo)志；體腔中卵母細(xì)胞無濾泡膜在线免费黄色高清网站，卵黃膜的形成與發(fā)育靠卵母細(xì)胞本身91伊人精品；卵徑小于60μm的卵母細(xì)胞呈圓形，卵徑大于60μm 的卵母細(xì)胞為卵圓形精品视频专区一在线观看，形態(tài)上成熟的卵母細(xì)胞短徑約115—120μm欧美激情男操女网站、長徑約140—145μm、卵黃膜厚7—9μmA级片。

The porine is the polyembryony animal, many obtains the ovocyte from the porine ovary although but the maturity quite to be low; Because the ROSI technology is does not have to grow the mature sole round sperm cell to pour into directly completely in the ovicell nature, jumped over the spermatozoon in to pass through the physiology and the biochemistry, if the ovicell nature mature or the activation degree were insufficient, added the round immature sperm cell maturity quite inferior reason, very possibly Causes the ROSI micro fertilization defeat.

豬是多胎動物99热在线观看精品免费，從豬卵巢上獲得的卵母細(xì)胞數(shù)量雖然較多，但成熟度比較低黑人黄色网站；由于ROSI技術(shù)是將沒有完全發(fā)育成熟的單一圓形精細(xì)胞直接注入卵胞質(zhì)內(nèi)jizzjizz日本在线，跳越了精子在穿過透明帶和卵質(zhì)膜等過程中所發(fā)生的生理和生化反應(yīng)；如果卵胞質(zhì)成熟或活化程度不夠日韩精品无码加勒比综合，加之圓形未成熟精細(xì)胞成熟度比較差等原因久久中国一级黄色片，很可能造成ROSI受精的卵母細(xì)胞內(nèi)部激活因子蓄積減少和生成不足，引起精核解聚困難群P网址、精圓核不能形成3p性交A片免费、第二極體不能排出、卵母細(xì)胞孤雌發(fā)育率增加等在线免费观看H片，使ROSI顯微受精失敗2021韩国三级在线无码中文。

Based on the technical maturity with an emphatieal considering economic maturing and on approprite considering of guantitative maturity, it determined the felling age of masson pine pulpwood on different site index and different grow model, the best manage model on different seed provenance and different site index; the felling ages of masson pine pulpwood in Hubei Province were from 15 to 26 years old.

根據(jù)"以工藝成熟為基限，重點(diǎn)考慮經(jīng)濟(jì)成熟国产国语对白不卡AV观看，適當(dāng)兼顧數(shù)量成熟"的原則免费黄色网站播放，確定不同立地蜜芽tv国产精品一级毛片、不同栽培模式下馬尾松紙漿林的主伐年齡及不同種源、不同立地指數(shù)的最佳經(jīng)營模式一级黄页电影免费在线大全，提出湖北省馬尾松紙漿林的主伐年齡在15～26 a之間特级毛片高潮一级一级毛。

Born Victor John Mature (to a German-speaking Tyrolean father, Marcellus George Mature, a cutler, and a Swiss-American mother, Clara Mature) in Louisville, Kentucky, Victor Mature worked as a teenager with his father as a salesman for butcher supplies.

出生維克多約翰成熟（一講德語的tyrolean的父親， marcellus喬治成熟亚洲91av网，潔西卡91精品自产拍不卡，以及瑞士，美國的母親克拉拉成熟）在肯塔基州路易斯維爾亚洲欧美一区二区视频，維克托成熟的工作一级黄色片日日爱，作為一名少年與他的父親作為一個(gè)推銷員，為屠夫用品91久久久久久一区二区。

The result showed that different concentration of FSH and LH were needed for sheep oocytes IVM in different season Xinjiang. FSH 12.9ug/ml,LH0.86IU/ml is proper to sheep oocytes IVM in summer-autumn anestrous period. FSH 8.6ug/ml,LH0.57IU/ml is proper to sheep oocytes IVM in autumn oestrous period.

結(jié)果表明：新疆綿羊卵母細(xì)胞在不同的季節(jié)中色黄乱婬伦免费网站耙，體外成熟所需的FSH、LH濃度不同黄片一区二区三区，夏秋乏情季節(jié)適宜于體外成熟的激素濃度為FSH 12.9ug/ml亚洲欧美视频一区，LH0.86IU/ml；秋季發(fā)情季節(jié)適宜于體外成熟的激素濃度為FSH 8.6ug/ml欧美日韩性生活，LH0.57IU/ml91AV导航站；春季乏情季節(jié)適宜于體外成熟的激素濃度為FSH 8.6ug/ml欧美插插影院，LH0.86IU/ml亚洲黄色在线观看网站。

The effect of EGF on the maturation of oocyte nuclear and cytoplasm is studied by adding EGF in culture media in vitro. In addition, it is also examined by parthenogenetic activation.

本試驗(yàn)在體外成熟培養(yǎng)液中添加EGF，研究其對核及質(zhì)成熟的作用欧美一区二区在线网站观看蜜臀，并通過孤雌激活來檢驗(yàn)EGF對牛卵母細(xì)胞胞質(zhì)的成熟免费黄色网址直接进入。

Most of EGFR in unmatured and matured oocytes was in variation, and there was obviously more expression of variation in matured oocytes than that in unmatured oocytes.

未成熟和成熟的卵母細(xì)胞中主要存在Ⅷ突變型，而野生型表達(dá)相對較少国际a片播放，成熟后卵母細(xì)胞中的Ⅷ突變型蛋白明顯增加欧美精品中文字幕在线视频。

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h轉(zhuǎn)為正常培養(yǎng)24h，不影響卵母細(xì)胞的成熟和孤雌激活能力78摸免费人成视频在线观看，并且CHX抑制后再成熟的卵母細(xì)胞經(jīng)孤雌激活發(fā)育到囊胚的比例與對照組卵母細(xì)胞相似国产AAA免费黄色视频。8、體外受精后免费人成a，CHX抑制后再成熟卵母細(xì)胞的多精受精現(xiàn)象顯著增加欧美性爱精品店，發(fā)育到桑椹胚/囊胚的比例顯著低于對照組。9黄色视频一、二、三区、CHX抑制過程中皮質(zhì)顆粒仍能發(fā)生遷移人人干人人射视频，但是CHX抑制會對皮質(zhì)顆粒的遷移造成不可恢復(fù)的損傷，使再成熟的卵母細(xì)胞內(nèi)皮質(zhì)顆粒不能完全遷移午夜福利120。10操东北妇女视频、CHX抑制對山羊卵母細(xì)胞α-微管和微絲都沒有影響色播影院性播影院午夜私人影院，無論是抑制后處于生發(fā)泡期的卵母細(xì)胞，還是抑制后再成熟的卵母細(xì)胞亚洲色图精品录音，微管和微絲的分布都與對照卵母細(xì)胞相似熟女乱伦91视频一区。

Pig oocytes cultured in vitro for some time were inseminated by frozen–thawed ejaculated sperm. At specified times after insemination, sperm penetration, cell cycle progression and mitogen-activated protein kinase phosphorylation were evaluated. It was shown that:(1) oocytes at various maturational stages could be penetrated by sperm;(2) sperm penetration did not affect meiotic cell cycle progression;(3) sperm penetration of germinal vesicle oocytes and maturing oocytes did not alter MAPK phosphorylation; and (4) when premetaphase I and metaphase I oocytes, in which MAPK was activated, were fertilised, no evident MAPK dephosphorylation was detected as in metaphase II oocytes.

不同成熟階段的豬卵與精子融合后體外培養(yǎng)發(fā)現(xiàn)：(1)不同成熟階段的豬卵母細(xì)胞都可被精子穿透；(2)精子穿透不影響減數(shù)分裂細(xì)胞周期進(jìn)程网站黄色在线；(3)精子穿透GV期卵母細(xì)胞和正在成熟的卵母細(xì)胞不改變MAPK磷酸化：(4)已受精的前中期I和中期I卵母細(xì)胞的MAPK有活性全免费A级毛片毛片，MAPK不像MII卵母細(xì)胞那樣受精后發(fā)生磷酸化。

LH plaies a role to delay the occurrence of germinal vesicle, prolong the maturation duration of cytoplasm, enhance the nuclear and cytoplasm synchronization of ovine oocytes matured in vitro, and finally improve the potentiality of their in vitro development.

LH可能通過延遲生發(fā)泡破裂和促進(jìn)胞質(zhì)成熟的方式国产一线天精品视频，使綿羊卵母細(xì)胞的核質(zhì)成熟趨于同步91嫩草国产线永久免费，有助于綿羊卵母細(xì)胞的體外成熟以及受精后的進(jìn)一步發(fā)育。