In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

為了研制副結(jié)核病敏感、特異的診斷試劑和新型男女男永久备用网站地址、高效的預(yù)防制劑哪个网站有偷拍视频，尤其是DNA疫苗，本研究篩選了M.paratuberculosis主要保護性抗原SOD基因阿v视频免费观看国产视99频，以M.paratuberculosis C-2染色體DNA為模板欧美一级a免费，以SOD基因的特異性引物進行PCR擴增，獲得了624bp的SOD基因久久久久久久91久久久，通過T-A克隆技術(shù)三级黄视频一区二区三，將PCR產(chǎn)物克隆至pMD18-T Vector中，以質(zhì)粒大小日批视频网站免费看、酶切分析欧欧美一级α爰高清成在线看、PCR擴增及序列分析鑒定重組克隆，成功地構(gòu)建出克隆質(zhì)粒pMD18-T-SOD91p0rn九色丰满熟女，序列測定及DNASTAR分析表明一级a爱片免费视频观看，所獲得的M.paratuberculosis C-2 SOD基因與Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，兩者核苷酸序列的同源性為99％直接观看黄色网站视频，氨基酸序列的同源性為99.5％，表明該基因在副結(jié)核分枝桿菌中是高度保守的久久产精品一区二区三区污。

For example, a healthy 18-month-old boy who "had no history of allergy or any prior postvaccinal adverse event" before receiving the chickenpox vaccine, was admitted to the intensive care unit four days later with a low platelet count.

例如欧美作爱牲交a，一個健康的18個月大的男孩在接種水痘疫苗之前&沒有敏感癥或者任何接種后會表現(xiàn)出來的有害現(xiàn)象&，但在接種之后卻因為血小板計數(shù)低而住進了加護病房四天欧美一极片。

I think your baby may be more sensitive to the vaccine, do not be too worried.

我想您的孩子可能就是對免疫疫苗比較敏感国产激情久久久久影院，不必太擔(dān)心。