Based on the previous literatures, the following major innovation works were carried out in this dissertation:(1) The derivatization reaction conditions of ephedrine and pseudoephedrine with sensitive derivation reagent in aqueous system were studied and two new methods were developed for the assay of them by microemulsion electrokinetic chromatography and micellar electrokinetic chromatography with LIF detection. The sensitivity and analysis times were greatly improved compared with the previous reports;(2) A new method of CZE with indirect LIF detection was developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside) with fluorescein as the probe. The proposed method enlarges the application range of LIF detector, and provides new approach for the analysis of certain compounds difficult to derivatize;(3) Based on the above research, an MEKC with indirect LIF detection method for the simultaneous determination of adenine and guanine in DNA extracts from fungus, maize and soybean was established;(4) A new method for the investigation of the complexes formed between human serum albumin and ampicillin sodium under the simulated physiology conditions using laser light scattering technique was developed.

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The proposed method surpassed other chromatographic alternatives in terms of limit of detection at least 10~3 folds with good reproducebility. Chapter 4 A capillary zone electrophoresis with indirect LIF detection method is developed for the simultaneous determination of six coumarin compounds (esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside).

第四章建立了一種毛細(xì)管區(qū)帶電泳—間接激光誘導(dǎo)熒光檢測法同時測定6種黃酮類化合物(秦皮甲素免费的又大又粗又爽的视频、秦皮乙素久久精品国产精五月婷、異秦皮定、染料木素1024手机看片欧美日韩你懂的、柚皮苷和槐角苷)的新方法亚洲欧美日韩一区在线，擴大了LIF檢測的應(yīng)用范圍。

The interaction between three small flavoind drug molecules, which are apigenin, galangin and genistein, and BSA is investigated with fluorescence spectroscopy using the drug's quenching on the endogenous fluorescence of serum albumin.

采用熒光光譜法，利用藥物對牛血清白蛋白自身內(nèi)源熒光的猝滅久久精品国产88久久综合，研究了芹菜素日韩视频一区在线播放、高良姜素和染料木素這三種黃酮藥物小分子與牛血清白蛋白分子間的相互作用，研究發(fā)現(xiàn)藥物分子與牛血清白蛋白分子之間發(fā)生了有效的靜態(tài)猝滅欧美黄动漫在线观看。欧美超级碰碰碰人妻。。

AIM: To improve the in vivo activity of genistein by increasing the solubility and permeability of genistein.

目的：通過提高染料木素的溶解度和滲透速率來改善染料木素的小腸吸收及體內(nèi)活性性VideOS欧美熟妇喷潮。

Further investigation of the macroscale preparation and especial the purification of genistein is still in progress.

對于染料木素的中試規(guī)模制備欧美肥婆性猛交XXXX，尤其是染料木素純化工藝條件的優(yōu)化進(jìn)行了深入探索。

The structures were elucidated by spectral analysis and chemical evidence.Result:Six compounds were isolated from the plant and identified as alpinumisoflavone,wighteone,daidzein,genistein,vittadinoside and stigmasterol.

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My company's main products: Rhynchophylline Gouteng silymarin (ethanol extract And no residual acetone) silymarin (water-soluble) silybin resveratrol daidzein emodin Suanzaoren extract of soy isoflavones curcumin, artesunate - Song Shupi extract, Hypericum perforatum extract, wild chrysanthemum extract, camptothecin, hawthorn extract, ursolic acid, corn required to extract, Maidong extract, garlic extract, ginsenosides, Chinese wolfberry extract - And asked Jing extract, green tea extract, the extract of small BAI Ju, Kushen extract, valerian extract, Salicylaldehyde glycosides, podophyllotoxin, Monascus Abulimiti of admission, puerarin extract, extract of astragalus, bitter melon extract Of, the thorn Tribulus terrestris extract, the extract of Ginkgo biloba leaves, Zhishi extract, Icariin, grape seed extract, Salvia extract yam extract, blueberry extract, the extract of Andrographolide, white Liu admission of Piti , Filed on Schisandra, Gynostemma extract, the extract of Angelica, Trifolium pratense extract, the extract of Rhodiola, soil Fritillaria saponins, Huang Chunju extract, and Cimicifuga extract, Hypericum perforatum Extract, Polygonum cuspidatum glycosides, genistein, reserpine, synephrine, sinomenine, Ciwujia glycosides, yam saponin, puerarin, peeling hormones, Kawa Ti of admission, lutein, lycopene, pumpkin seeds extract, licorice extract, nettle extract, the extract baicalin, Osthol, Asarum, saw Mr Brown extract, water Zaojiao extract, rutin extract, purple cabbage extract , Motherwort extract, Yanhusuo extract, hops extract, the extract of Magnolia officinalis, Cyclovirobuxine wood extract, Ban Langen extract, ginger extract, dandelion extract, senna extract, aloe extract , Dangshen extract, the extract of honeysuckle, quercetin, etc.

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Result: Eleven chemical constituents were isolated and elucidated as arctiin,phillyrin,forsythiaside,liquiritigenin,liquiritin,genistein,formononetin,daidzein,glycitrin,3,3′,4-tri-omethlellagic acid and chlorogenic acid.

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Specializing in natural plant research and development, production, sales and biosynthesis of the high-tech enterprise as a whole, the existing products: ursolic acid, luteolin, kaempferol, shikimic acid, salicylaldehyde glycosides, genistein, nuciferine , matrine, oxymatrine, quercetin ...

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