Reactive oxygen/nitrogen species production was measured with 2',7'-dichlorodihydrofluorescein diacetate dye.

活性氧或氮類產(chǎn)物用雙乙酸鈉進(jìn)行染色測量，蛋白酶-9和-12的活性用熒光酶抑制劑檢測。

Methods: After transferred pcDNA3.1/hTM plasmid into rabbit artery by high-pressure injection, rabbit common iliac artery were cut and anastomosed again. At the 14 days and 28days after second operation, we checked inside diameter of anastomotic stoma and blood flow velocity by color Doppler. The treated artery were sliced and stained by Verhoeff. Local neointima formation and the ratio stenosis of intervascular were calculated by computer.

用注射式加壓轉(zhuǎn)染的方式對兔動(dòng)脈壁轉(zhuǎn)染pcDNA3.1/hTM質(zhì)粒精品天堂很干视频，再制造動(dòng)脈損傷-阻滯模型99热在线这里只有精品3，于術(shù)后14天、28天用彩色多普勒觀察活體吻合口內(nèi)徑和血流流速CaoPoron草棚在线视频；再做病理切片Verhoeff染色久久视频这里只有久久视频，觀察血管內(nèi)膜增生的程度、部位免费进入黄色网站，計(jì)算血管內(nèi)膜面積四虎成人入口在线观看网站视频、中膜面積和血管狹窄率。

Methods SECs were isolated by collagenase perfusion combined with isopycnic centrifugation in a two-step percoll gradient and selective adherence, and identified by morphologic observation under light microscope, by immunocytochemistry for CD14 and Ⅷfactor related antigen, by immunofluorescence staining for RECA-1 and fenestrations under SEM.

方法用膠原酶灌注結(jié)合Percoll密度梯度離心加選擇性貼壁的方法分離SEC日韩一区二区在线视频观看；用光鏡形態(tài)學(xué)免费黄色网业在线看、Ⅷ因子和CDl4免疫細(xì)胞化學(xué)染色及RECA.1單抗間接免疫熒光法及SEM鑒定SEC；采用TEM及AnnexinV聯(lián)合PI雙染的方法檢測SEC的凋亡国产精品视频一区二区三区无码乱伦。

After 3 days treatment with RA, the cells began to show phenotypical changes, assuming a neuronal-like morphology with contemporaneous formation of dendritic-like structures.

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At the end of 12 and 18 weeks, rats were killed, taken the muscle of posterior limb, after separating the gastroenemius国产色清、soleus曰批全过程免费视频在线观看直播、plantaris, to prepare the frozen section of the each kind of muscle respectively to be confirmed to form the myopathy model by the histochemical stain.(3) RT-PCR、Western blot and immunohisto-chemistry stain were respectively used to detect the expression of NF-κB作爱超A级黄色视频、MMP-2 and MMP-9 during different period.

2實(shí)驗(yàn)組用酒精給大鼠灌胃欧洲A片网址，對照組用含與酒精體積相等的生理鹽水灌胃，兩組均喂飼改良的全營養(yǎng)高脂飼料欧美日韩在线观看二区视频，于12周及18周后国产哟一区二区三区视频在线看，取后肢肌肉，在分離出腓腸肌自拍视频偷拍天堂、比目魚肌国产三级精品三级、跖肌后，將各類肌肉均分別地制備成冰凍切片和石蠟切片五月综合色色，經(jīng)組織化學(xué)染色確認(rèn)成模思思99re6精品视频免费观看。

Methods 24 SD rats were randomly divided into normal group,model group,aminophylline treatment group(35 mg/kg), 8 rats in each group. The bronchial asthma model in rats was built by egg protein sensibilization and longterm inhalation provocation. The rats in treatment group were lavaged each day from the first time of provocation.After 4week treatment, the rats were killed to obtain lung tissue for staining with HE.

24只SD大鼠隨機(jī)分為正常組、模型組国模AⅤ私拍一区二区、治療組最新在线国产，每組8只偷拍视频区一，除正常組外以卵蛋白致敏并吸入激發(fā)法制備大鼠哮喘模型，治療組一级特黄AAA片免费女、模型組從第1次哮喘激發(fā)開始(造模第3周)分別給予氨茶堿亚洲爆乳性爱三级片、0.9%氯化鈉溶液灌胃給藥，1次/d国产精品一在线观看，用藥4周處死大鼠孕妇国产精品免费视频，取肺組織HE染色，用彩色圖像分析儀測量支氣管壁面積美女黄色国产网站、支氣管平滑肌面積色a4yy，用ELISA雙抗體夾心法測定肺組織MMP9和TIMP1含量。

Then animals were killed by gas embolism, aorta was separated from the beginning of aortic arch to abdominal aorta crotch. At the end of experiment, the aortas were prepared for measuration of the concentration of ceramide and cholesterol. The pathological changes and plaque area were measured.

第十周實(shí)驗(yàn)結(jié)束時(shí)销魂美女一级毛片，用氣栓法處死動(dòng)物，將主動(dòng)脈全部取下私人福利影院，沿正中線剪開美女黄网在线视频免费观看，攝像，同時(shí)作主動(dòng)脈斑塊面積分析及HE染色等主動(dòng)脈病理學(xué)檢測欧美激性，并取血管組織用HPTLC測量血管膽固醇的含量国语对白偷拍偷窥。

Methods After treated with a specific demethylating agent,Aza and acetylating agent, TSA, the status of 5'CpC island methylation of ING1b gene in HT29 human colon cancer cell line was analyzed using methylation specific polymerase chain reaction,and the level of histone acetylation was analyzed by chromatin immunoprecipitation,and reverse transcription polymerase chain reactionwas used to examine ING1b mRNA expression.

應(yīng)用特異性DNA甲基轉(zhuǎn)移酶抑制劑5-氮-2'-脫氧胞苷（5-Aza-2'-dc，以下簡稱Aza）及組蛋白去乙趺琅徊俪鏊叱蓖荆化酶抑制劑曲古抑菌素A作用人結(jié)腸癌細(xì)胞株HT29后免费A片视频日本一区二区三区，用甲基化特異性PCR檢測該ING1b基因核心啟動(dòng)子區(qū)域CpG島甲基化情況，用染色質(zhì)免疫沉淀檢測其乙豕粕悠得夥压劭矗化組蛋白綁定的DNA情況人人爱人人操怕怕，并用逆轉(zhuǎn)錄聚合酶鏈反應(yīng)檢測ING1bmRNA表達(dá)。

Activity of alkaline phosphatase and ability of formation for calcifying nodules were determined.

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Methods Forty mice were randomly divided into 4 groups: one normal control group,mice in the other three groups were all infected each with 25 cercariae, the infected control group was fed for 10 weeks after infection, and 2 weeks after infection, the high dose PTX group was given PTX 360 mg/ for 8 weeks and the low dose PTX group was given PTX 180 mg/also for 8 weeks.

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