Or cytologic examination, the samples were prepared using standard techniques. Sample for FCM analysis were centrifuged and exposed to hypotonic solution cotaining detergent and propidium iodide. Thirty-eight patients had pleural effusion due to benign disease, whilst 33 patients had primary lung cancer. All 38 patients with benign pleural effusions showed FCM diploidy.

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Shenyang Research Institute of Chemical Industry, Shenyang 110021, China Abstract: Spectrophotometric analysis showed that the peak absorbance for food dye of Allura Red and biological dye of Ponceau-G occurred at 501 and 510 nm. In sunlight after 2, 4, 6, 8, 10 hours, the decomposition rates of Allura Red and Ponceau G were between 2.4 to 5.8% and 3.4 to 10.8%, so they are stable in sunlight. The recovery rates on cottons and glasses were very high, so they are easily recoveried from targets. Further studies showed that the recovery rates of two dyes on the leaves of wheat, cabbage, cucumber and tomato were different, so it need previously determine the recovery rate of tracer on the target crop to adjust the result. Field experiment showed it was easy to measure the pesticide spray distribution of high volume spay and low volume spray to use Allura Red and Ponceau-G as tracers.

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Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通過本次課題，我們發(fā)現(xiàn)用2%NaOH-NALC消化液可在10分鐘內(nèi)迅速裂解痰液的粘性介質(zhì)又粗又黑欧美硬大视频，在保證消化效果的同時(shí)不會由于NaOH過強(qiáng)的作用殺死細(xì)菌（時(shí)間控制在20分鐘內(nèi)）天天一级欧美，亦不會影響磁性顆粒的作用；通過應(yīng)用自制定量模擬臨床標(biāo)本，我們比較了抗酸染色和金胺O熒光染色的檢出限无码理论在线观看，金胺O熒光染色黑暗背景下發(fā)出明顯綠色亮光的結(jié)核桿菌較抗酸染色白色背景中的紅色結(jié)核桿菌更容醒目免费看乱女伦一级A片，金胺O熒光染色的檢出限在0.05~0.001個(gè)麥?zhǔn)蠁挝恢g，高于抗酸染色的0.05個(gè)麥?zhǔn)蠁挝还鷅d在线，前者敏感度較高欧美精品黄网站在线播放；另外，經(jīng)過磁性納米顆粒富集作用后的標(biāo)本涂片欧美人与拘交A片视频网站，在鏡下顯示其結(jié)核桿菌數(shù)量明顯高于未經(jīng)過富集的標(biāo)本AAA黄色片段视频。

Hematoxylin and eosin, Alcian blue, Masson Trichrome, and Elastica Van Giesonstains were performed for each LF. Nine LF were collected and were used for biologic study of real time RT-PCR to quantitatively measure mRNA expression of Type I collagen and elastin in each LF.

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Methods Acanthamoeba polyphaga and Legionella pneumophila were co-cultured under laboratory condition. At consecutive time points during the culture, smears of the cultured products were made on glass slides for staining purposes. Different types of stainings including Gram′s staining, Gimenez staining, Giemsa staining and immunofluorescence were used to determine the best method for the identification of amoebal pathogens.

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The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

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A method of dyeing a polyamide fiber structure characterized by using a polyamide fiber structure which has structural differences along the fiber length direction and dyeing the structure with an anionic reactive dye in a dye bath having a pH of 3 to 8; and a dyed polyamide fiber structure obtained by the above method.

本發(fā)明的聚酰胺類纖維構(gòu)成物的染色方法，是以用纖維縱向上有結(jié)構(gòu)差別的聚酰胺類纖維構(gòu)成物在线欧美亚洲综合区导航，經(jīng)將pH調(diào)至3～8的含陰離子性反應(yīng)性染料的染液染色為特征的偷拍妹子综合网，本發(fā)明的染色物是由該染色方法制得的聚酰胺類纖維的染色物。

In this paper, The influence of heat set and dyeing on the structure of PTT fiber was investigated by FT-IR, DSC, critical dissolution time. The influence of heat set on the dyeability of PTT fiber was discussed, too. Meanwhile, many experiments on the dyeing performance of PET fiber were carried out. The effect of different dyeing temperature on the uptake and visual color depth value and dyeing characteristics of different dyes with temperature increasing were discussed, and the influence of temperature on the dyeing depth were also evaluated when dyeing for PTT and PET fiber in the same dye bath condition. Especially the kinetics and thermodynamics of dyeing with disperse dyes were mainly studied.

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objective to investigate staining methods for two-dimensional gel(2-de)electrophoresis in multidrug resistance of gastric cancer.methods cultured vincristine-resistant human gastric cancer cell line sgc7901/vcr and its parental cell line sgc7901.variant amount protein of those cells were separated by 2-de.gels were stained with silver nitrate or colloidal coomassie brilliant blue,and scanned by image scanner.results well-resolved,reproducible 2-de patterns of sgc7901/vcr and sgc7901 were established.silver staining was better when protein sample amount was low,overloaded protein will interfere resolution of the maps.gels stained with colloidal coomassie brilliant blue had more protein spots numbers and abundance without apparent trails when increased loading protein sample.conclusion two staining methods were influenced largely by the sum of protein samples,properly selection may be helpful for further study with proteomics in multidrug resistance of gastric cancer.

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Through this topic, we discover with the 2%NaOH-NALC digesting fluid may in 10 minute in rapid decomposition sputamentum resisting medium, during guarantee digestion effect not because the NaOH strong function will have killed the bacterium (timing control in 20 minutes), also will not affect the magnetic pellet the function; Through the application self-restraint quota simulation clinical specimen, we have compared the acid-fast stain and the pyoktanin yellow O fluorescence dye pick out limit, the pyoktanin yellow O fluorescence dyes the dark background to after-crop the obvious green bright the bacillus tuberculosis to compare in the acid-fast stain white background the red bacillus tuberculosis to accommodate strikingly, the pyoktanin yellow O fluorescence dyes picks out limits between 0.05~0.001 Mai unit, is higher than the acid-fast stain 0.05 Mai unit, the former sensitivity is high; Moreover, concentrates the function after magnetic nano particles the specimen smear, under the mirror demonstrated that its bacillus tuberculosis quantity is higher than the specimen which obviously has not passed through concentrates.

通過本次課題，我們發(fā)現(xiàn)用2%NaOH-NALC消化液可在10分鐘內(nèi)迅速裂解痰液的粘性介質(zhì)日韩欧美一级特黄大片视频，在保證消化效果的同時(shí)不會由于NaOH過強(qiáng)的作用殺死細(xì)菌（時(shí)間控制在20分鐘內(nèi)）黄页精品在线视频，亦不會影響磁性顆粒的作用；通過應(yīng)用自制定量模擬臨床標(biāo)本欧爱爱爱网，我們比較了抗酸染色和金胺O熒光染色的檢出限欧美国产综合，金胺O熒光染色黑暗背景下發(fā)出明顯綠色亮光的結(jié)核桿菌較抗酸染色白色背景中的紅色結(jié)核桿菌更容醒目，金胺O熒光染色的檢出限在0.05~0.001個(gè)麥?zhǔn)蠁挝恢g网站黄se，高于抗酸染色的0.05個(gè)麥?zhǔn)蠁挝蝗似蘧肥悠狄磺罢呙舾卸容^高；另外国产在线无码制服丝袜无码知名国产，經(jīng)過磁性納米顆粒富集作用后的標(biāo)本涂片欧美熟妇肉体销魂孕妇，在鏡下顯示其結(jié)核桿菌數(shù)量明顯高于未經(jīng)過富集的標(biāo)本。