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Methods: The specimens of peripheral blood were collected from 15 individuals and each specimen was divided into three parts, one processed in an hour and the other two stored at -80℃ and -20℃ respectively Two different reagents (TRI Reagent and TRI Rcagent-BD) were used to extract RNA directly from whole blood followed by reverse-transcription polymerase chain reaction for amplifying 3-Actins gene. The RT-PCR products were detected by the ultraviolet transilluminator after 1.5% agarose gels electrophoresis and being stained by ethidium bromide.

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The samples were prepared and stained with modified Mallory's trichrome staining and observed under light microscope.

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Trypan blue exclusion was used to measure the effect of TCS on cell growth and flow cytometry was used to detect the effects of TCS on cell apoptosis and cell cycle.

用臺(tái)盼籃染色法檢測TCS對細(xì)胞生長的影響国产午夜福利在线播放92,應(yīng)用流式細(xì)胞術(shù)檢測TCS誘導(dǎo)細(xì)胞凋亡的情況及對細(xì)胞生長周期的影響亚洲三级片视频。

Methods:The expression of mast cell tryptase and chymasec were examined in 37 cases of normal uterus with immunohistochemical double labeling techniques.

選取37例正常子宮組織,用雙重免疫組化染色法檢測肥大細(xì)胞中類糜蛋白酶和類胰蛋白酶的表達(dá)无码一级毛片免费。

Is highly debated. Microscopic studies using DMACA staining revealed flavanols in shoot tip cells of Tsuga Canadensis during the early anaphase and in prophase nuclei.

用DMACA染色法的顯微研究表明在在加拿大鐵杉莖尖細(xì)胞分裂后期的早些階段及細(xì)胞分裂早前期的細(xì)胞核中都有黃烷醇存在一区二区强奸。

When total protein was assayed by 2-D PAGE andstained with sliver, about 2000 protein spots with a pI of pH 3-10 and Mrof 12-120 kDa could be detected. All these protein spots were excised foridentification, among which 213 spots corresponding to 192 differentproteins were identified undoubtful by searching the mass spectra againstthe SwissProt/TrEMBL database.

我們利用雙向凝膠電泳對小鼠卵巢蛋白進(jìn)行了分離,在考馬斯亮蘭染色后將所有可見的蛋白點(diǎn)切取下97干视频,經(jīng)胰蛋白酶消化以后用MALDI-TOF質(zhì)譜儀進(jìn)行鑒定欧美一级俄罗斯a片。

Results ET-1 increased the synthesis of soluble type I collagen in different periods besides the 24h group, and had no effects on the type III collagen; immunohistochemical staining revealed that ET-1 also increased the synthesis of unsoluble type I collagen.

結(jié)果 ET-1較對照組除24小時(shí)時(shí)相外,均有不同程度的促HDPC可溶性I型膠原合成作用欧美色图亚洲,但對III型膠原作用不明顯综合sese;經(jīng)免疫組化染色證實(shí):ET-1尚可同時(shí)促進(jìn)細(xì)胞非溶解性I型膠原合成国产熟女精品视频500。

Methods:After diagnosis of varioliform gastritis was made by gastroscopy,biopsy was taken,regular pathological examination was made.

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METHODS: Twenty pationts with spinal tuberculosis were treated by anterior partial vertebrectomy of the diseased vertebrae, iliac grafting and internal fixation via anterior of posterior approach.

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The study ~s conducted to elucidate the mechanism by which the expression of adhesion molecules on human endothelial cells ~s up-regulatecL Methods Human endothelial cells derived fium umbilical venisQ-IUVEC were coincubated in viny whh native human sennn albtuiiin, HSA modified v~ith advanced glycation end products or RPMI-1640 culture medium. The expression of adhesion molecule intereellular adhesion molecule-l(7ICAM-l), vascular cell adhesion molecule-l and E-selectin was determined by irnrnunofluorescen staining and flow cytometer analysis.

分離正常人臍靜脈內(nèi)皮細(xì)胞,將晚期糖基化終產(chǎn)物修飾的人血清白蛋白欧美男女性爱视频中文免费、人血清白蛋白以及RPMI-1640完全培養(yǎng)基與HUVEC在體外共同培養(yǎng)18未成禁片一级毛片喷水,用熒光單克隆抗體染色,流式細(xì)胞儀定量檢測內(nèi)皮細(xì)胞表面細(xì)胞間粘附分子-1(ICAM-1)欧美午夜性、血管細(xì)胞間粘附分子-1(VCAM-1)免费视频在线观看4444、E-選擇素的表達(dá)。

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