Methods Establishing a model of whole cerebral ischemia-reperfusion in rats; examining the expressions of TrkA and NGF on cochleas assuming the method of immunohistochemistry stain; and examining the time and distribution of the cochlear hair cell apoptosis through the terminal deoxynucleotidyl transferasemediated dUTP nick end labeling.

建立大鼠全腦缺血再灌注模型欧美精品性XXXX，采用免疫組織化學染色方法檢測TrkA和NGF在耳蝸中的表達；用TUNEL法檢測耳蝸毛細胞發(fā)生凋亡的時相和分布。

Thirty cases (60 sides) of the hippocampus cortex in the cerebrum of human fresh copses from fetus (6 months) to children (old 6 years) were stained by AKP , and then observed and taken enlarged pictures were taken under microscope. All epillaries on these photoes were measured by vernier caliper and "ratio of volume" of the capillary were calculated by new method.

采用 30 例(60 側)6 個月胎兒至 6 歲兒童新鮮尸體大腦，用堿性磷酸酶染色www.αeαe.28.com日韩黄色片，在光鏡下觀察毛細血管分布，顯微放大照相国产模特AV私拍大尺度，自動彩擴機擴印照片国产进出又黄又大又粗视频，游標卡尺測量并計算海馬皮質毛細血管&比容積&。

Methods The human corneas in different ages were detected by using immunohistochemical techniques for 5 types of monoclonal antibody.

采用免疫組化法在线看羞羞视频网站，用5種單克隆抗體對不同年齡的人角膜切片進行免疫組化染色欧美性爱十八岁以下禁止进入，觀察人角膜的免疫狀態(tài)。

Methods Macrophagea were cultured in 6-hole plate and coverslip was prepared. Then macropages were cultured one day with different concentrations(0, 12.5, 25.50, 100, 200 mg/L) of matrine, oxymatrine and sophordine; the expression of CD91 and CD13 by immunohistochemical dyeing was analyzed by Image-Pro plus system (Media Cybemetic, Inc., 6.0 edition).

(1)用6孔板培養(yǎng)巨噬細胞RAW264.7中文字幕在现观看，制作細胞爬片，然后與不同濃度(0无码肏屄黄色视频、12.5十八禁www、25、50久久免费看A级毛片、100五月丁香婷中文、200 mg/L)的苦參堿、氧化苦參堿和槐定堿共培養(yǎng)超碰动态图，細胞爬片免疫組化染色日本欧美国产中文字幕，檢測CD91和CD13的表達情況，并采用Media Cybernetic欧美一欧美视频在线电影视频、公司的圖像分析系統(tǒng)進行定量分析色五月先锋无码中文字幕，測量積分光密度值。

After 3-8 passages, the oral CAFs and the normal mucosa fibroblasts from tissue culture were observed under light microscope. Vimentin,α-SMA and Cytokeratin protein were detected by immunohistochemistry SP method on glass coverslips. VEGF, TGF-β1, MMP-9, Tenascin-C mRNA were analyzed by semi-quantitative RT-PCR.

用組織塊培養(yǎng)法獲得原代CAFs和正常粘膜成纖維細胞在线涩视频网址，0.25%胰蛋白酶消化傳代和純化細胞456亚洲人成高清在线观看；通過形態(tài)學觀察和免疫細胞化學染色對口腔CAFs和NMFs進行鑒定：采用半定量RT-PCR檢測口腔CAFs和NMFs 4種基因的表達。

The rats were killed by cutting theirnecks and physical blood was taken for preparing serum, interleukin 2 (IL-2) was detected by radioimmunoassay ; the spleen was taken for measuring ConA-induced reaction of lympho- cyte proliferation and induced production of IL-2; and the activity of induced production of spleen's IL-2 was determined by the means of cell proliferation of mouse thymus; the skin and trachea were taten for slice with paraffin wax, dyeing with methybenzoamine blue so as to survey mast cells .

將大鼠斷頭留取軀干血制備血清裸体美女扒尿口喷白浆草莓视频，用RIA法檢測白細胞介素2（IL-2）人人爽人人操97；取脾臟行ConA誘導的淋巴細胞增殖反應測定和IL-2誘生；并用小鼠胸腺細胞增殖法檢測脾IL-2誘生活性欧美成A人片免费观看久久；取皮膚和氣管做石蠟切片黄A色片、甲苯胺蘭染色檢測肥大細胞，結果顯示：應激組較對照組脾淋巴細胞增殖降低国产免费乱在线观看，cpm分別為9466±3.17和24019±3879（P.001）十八岁以下禁止观看的黄；MC明顯減少肏屄自拍视频，分別為8.6±3.0/HP和25.4±3.7/HP（P.001），前組形態(tài)異常較其甚（MC多較小自拍视频日韩在线视频观看网站免费，呈多角或不規(guī)則狀）18禁三级黄色网站，且組織中可見分散的崩解顆粒。

Methods: Neuroepithelial stem cells derived from the embryonic neural tube of GFP transgenic mice were cultured and depurated in free serum medium containing B27, bFGF and EGF, and then they were directionally transplanted into the striatum of rats.

將GFP轉基因鼠的胚胎神經(jīng)上皮干細胞置于無血清培養(yǎng)基內純化擴增日本三级黄在线观看，用巢蛋白免疫組織化學染色鑒定神經(jīng)干細胞日本青草久久99热精品一区，將擴增后的含GFP基因的神經(jīng)干細胞在立體定位儀下，定向移植到大鼠的紋狀體內欧美特级AAAA。

In this experiment, a new method was used to purify the toxin protein produced by Verticillium dahliae. The supernatant of fungus culture was frozen and dried with Lyophilizer first, and then dialysed by Dialysis Membranes (MWCO 1000) after dissolved in distilled water. This method can eliminate the salt and sucrose in culture medium and reserve the protein component almost completely. VD-toxin were analysed using sodium dodecyl sulfate polyacrylamide gel analysis. The results indicated that the protein components were very complex, and included glycoprotein within 35.8kDa-83.2kDa. Furthermore, treatments on glycoproteins with high temperature, conA and proteinase alleviated, not abolished, their activities.NO and H_2O_2 production were assayed in two cultivars of cotton cells which were treated with VD-toxin.

本實驗首先確定了用冷凍濃縮后透析的方法初步提純棉花黃萎病菌毒素亚洲色图欧洲色图激情文学，該方法能有效去除粗提毒素中的蔗糖和鹽離子并能最大程度的保留黃萎病菌分泌的蛋白成分；對毒素蛋白成分鑒定結果表明蛋白條帶多又粗又大又色视频网站，每種蛋白的含量少九九国产黄色视频上传在线观看，小分子量蛋白含量多，糖蛋白染色結果顯示使劲摸使劲操无码在线，毒素蛋白中分子量在35.8kDa-83.2kDa之間的蛋白大多數(shù)為糖蛋白www.天天干.com，分子量小于35.8kDa的蛋白多為非糖蛋白；性質鑒定結果顯示污污美女在线观看免费，毒素中有活性的蛋白成分具有部分可耐高溫高壓黄色电影视频网址，且毒素蛋白中的蛋白成分和糖基成分都具有致萎活性。

Owing to different structures of cotton fiber and chitin fiber ,they have different adsorbability to reactive dyestuffs.

采用不同的醛類化合物對甲殼素纖維上的氨基進行保護亚洲一区IGAO影院，活性染料染色后用稀酸脫去氨基的保護基播放在线你懂。

You can use that water to dye fibers.

接著你就可以用這些又顏色的水染色了。