Detect that whether the ADSCs were differentiated inducedly into osteoblasts or adipogenic cells by Alkaline phosphatase staining日操av在线播放、Von kossa staining and Oil red "O"staining after the ADSCs were cultured in inductive basic medium containing osteogenic induction agent and adipogenic inducers for four weeks.②.

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RESULTS: After differentiation of human adherent myoblasts by neural induction medium, no cells with a neural cell morphology (ie., small, refractile cell body with dendritic cell extensions) were seen. All remaining myoblasts cultured with neural induction medium, myoblasts with proliferation medium and myotubes with differentiation medium containing 20 mL/L HS were positive for β Tubulin Ⅲ. C2C12 myoblasts were negative for β Tubulin Ⅲ. In contrast, all the above cells were negative for the markers Neurofilament Mr 68×103 and GFAP.

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Methods: Fresh stools of the patients suffered from diarrhea were examined with direct wet-mount, iodine staining and trichrome staining.

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The findings of the newly identified goose paramyxovirus strain GPMV/HBexperimental infection were as follows: The challenge caused high morbidity and highmortality.The clinical signs included severe depression, decreased appetite or anorexia,white,yellow and green diarrhea, with neural symptoms;pathological changes showed thathaemorrhage and ulceration of intestine mucosa, necrosis of spleen and pancreas,andedema of liver and kidney, uratic deposition in kidney.Eosinophilic inclusion bodies werefound in the cytoplasm of pancreas and a large quantities of fiber protein were found inintestine. Lots of hemosidefin was found in some area of liver and kidney. A number ofT、B lymphocytes reduced sharply in spleen,lymph nodes in dead geese. Transmissionalelectron microscope showed that some cells had the morphological characteristics ofmitochondria swelling, morphological changes were condensation of nuclear chromatininto dense masses,followed by its marginating against the nuclear envelope, andespecially concentration of cytoplasm association with lots of lipid droplet.

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The cochleae of animals were dissected. propidium iodide, a dna intercalating fluorescent probe, was used to trace the morphological changes in ohc nuclei. f-actin staining was used to determine missing ohcs. the single strand dna in apoptotic ohcs of guinea pigs and mice, and apoptosis inducing factor and endonuclease g in ohcs of guinea pigs were examined by immunofluorescence method.

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Yunnanensis. The pollens of dehisced anthors of flowering P. polyphylla plants was slightly painted on the slide glass, the activities of P. polyphylla pollens were determined resp. by iodine-potassium iodide dyeing method (I2-KI), triphenyl tetrazolium chloride staining method, aceto carmine dyeing method and fluorescein diacetate method with a little improvement.

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Methods:Based on the model of human umbilical vein endothelial cell,this study adopted Rose Bengal Stain, cell ELISA, immunocytochemistry techniques to investigate the effect of berberine on lymphocyte-endothelium adhesion and the molecular mechanism.

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The process for dyeing superfine polyester fabric with disperse dye in alkaline bath was studied.

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The article mainly probes and researches the dyeing technology condition of polyester and cotton fabric with dispersing /reactive dye in one bath .

探討了用分散/活性染料對(duì)滌/棉混紡織物進(jìn)行一浴法染色的工藝條件，以及用載體法對(duì)滌/絲混紡織物進(jìn)行一浴一步染色99色免费。

When we begin to the medicine, urine protein was measured in urine volume of 24 hour the every two day. 4. Specimen collections and laboratory check: In the 21 days of pregnant rats, infants were taken which registered weight and height after aesthesia. Cr and BUN in the blood were measured.

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