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METHODS摘要: Otocyst epithelia surgically isolated from fetal rats were cultured and passaged. Proliferation and differentiation of cultured cells were observed using BrdU labeling to find the progenitors. Immunocytochemistry (Factin, cytokeratin and calretinin) was used to detect cell properties of cultured cells.

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Methods: For 66 cases whose bone marrow showed hypocellularity in BM aspirate smear, trephine biopsy and reticulin staining were done at the same location in the same patient and at the same time as the BM aspirate smear was done.

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In this thesis, rat-tails were used as experiment objects, there are 40 slices including transverse and longitudinal, HE-dyed and undyed. Excited by femtosecond laser, the changes of backward SHG and TPEF versus sample preparation, excitation wavelength, excitation power, penetration depth and so on were observed by two-photon laser scanning confocal microscope, effect factors of backward SHG and TPEF and differences of both were discussed and compared, and related comments were also presented.

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Stained with 0.4% trypanblau for 4 min, then counted the stained cells and unstained cells.

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Eight kittens were deduced amblyopia by monocular chronic atropinization; four (2 normal and 2 amblyopia) kittens were perused in every three weeks. Changes in number and optical density of immunopositive neurons of the expression of VIP-protin and VIP-mRNA in the visual cortex area 17 were analyzed with immuno-cytochemistry, using the technique of in situ hybridization and SABC.

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