Methods Genomic DNA samples of two isolated Plasmodium falciparum isolate strains prepared directly from 5 cases of cerebral malaria patients′ blood in mengla County, Yunnan Province and in Yingjiang County, Yunnan Province were used for polymerase chain reaction amplification and the two pairs of oligonucleotides for the highly conserved genes encoding FC27 merozoite surface protein 2 (MSP2) of Papua New Guinea strain of Plasmodium falciparum were used as primers. The PCR products were digested with BamH1 and Hind Ⅲ respectively, and the generated fragment MSP2 were cloned into M13mp18 and M13mp19 vectors and their DNA was analyzed as the templates for DNA sequencing by the dideoxy chain-termination method.

應(yīng)用多聚酶鏈反應(yīng)對(duì)5例中國腦型瘧患者惡性瘧原蟲云南省勐臘縣勐罕分離株和云南省盈江縣農(nóng)場(chǎng)分離株基因組裂殖子表面蛋白2(MSP2)基因進(jìn)行擴(kuò)增国内99在线，將擴(kuò)增產(chǎn)物分別經(jīng)BamHI和Hind Ⅲ雙酶切后，回收的MSP2基因分子定向克隆M13mp18和M13mp19載體自拍偷区亚洲综合精品，按Sanger雙脫氧鏈終止法進(jìn)行DNA序列測(cè)定毛片网官方，并與惡性瘧原蟲株FC27、K197无码在线观看、IC1和CAMP株進(jìn)行同源性分析比較91美国黄色視頻。

Results The recovery rate of three representative bacteria determined by the culture media dilution method, were more than 70% and the recovery rate of two representative mold and fungus determined by the membrane filtration methods with 1000 ml washed, were more than 70%.

結(jié)果 采用平皿法中的培養(yǎng)基稀釋法測(cè)定樣品人工污染3株細(xì)菌代表菌株的菌回收率均于70%，采用薄膜過濾法在沖洗1 000ml時(shí)乱肏视频，測(cè)定樣品人工污染2株真菌代表菌株的菌回收率均大于70%级特大黄片视频影院国产。

Strains of fungi were detected, among them, 62 (71.3%) were Monilia, 17 (19.5%) mold fungus, and 7 (8.0%) budding fungus. The most common Monilia were Monilia albicans, slick Monilia and Oidium tropicale.

感染菌譜中真菌87株，其中念珠菌62株(71.3%)18岁禁污网站，毛霉菌17株(19.5%)中国一级A片XXXX，酵母菌7株(8.0%)，念珠菌中常見的菌種依次為白色念珠菌黄网占在线、光滑念珠菌和熱帶念珠菌能看av的网站。

Methods Genomic DNA was extracted from 50 strains of S.schenckii, including 49 clinical isolates and 1 referrence strain, as well as from 1 strain of Mucor, Aspergillus fumigatus and Candida albicans, respectively, as controls. Four primers were designed according to previous studies, and screened by PCR.

以50株不同地區(qū)來源的孢子絲菌臨床分離株及標(biāo)準(zhǔn)株的基因組DNA作為研究樣本，以毛霉免费一级a片在线播放视频、煙曲霉免费看av的在线网站、念珠菌臨床分離菌株基因組DNA作為對(duì)照，通過特異引物PCR擴(kuò)增的方法篩選國內(nèi)外文獻(xiàn)中報(bào)道的4對(duì)孢子絲菌引物黄色三级人人操。

In order to compare the homology of the gene encoding VP2 between MDPV-Q and MDPV which was registered in the GenBank, also in order to find out changes of the VP2 gene and the immuno-genicity between the wild-strain MDPV-Q and the attenuated strain MDPV-26 which derived by continued passage of virulent wild-type in muscovy duck embryo, a pair of primer (LHMP7/LHMP8) was designed. The upper one LHMP7 and the lower one LHMP8 corresponded to the MDPV-specific nucleotides sequence 2885-2900 and 4618-4604 respectively, according to the MDPV nucleotide sequence databases. The length of the sequence embraced by the primers was 1734bp.

為了獲取這一基因片段與國外分離株進(jìn)行同源性比較九九激情视频在线看，同時(shí)也為了了解MDPV強(qiáng)、弱毒株VP2基因之間的異同關(guān)系日韩激情中文字幕视频在线，及其免疫原性的變化規(guī)律国产亚洲黄色录像，通過DNA重組技術(shù)，設(shè)計(jì)了一對(duì)引物L(fēng)HMP7／LHMP891日韩在线视频免费观看，該對(duì)引物選取位于2885～2900及4618～4604的兩段序列国产一区二区三区久久，跨幅為1734bp，并在這兩條引物中分別加入兩種限制性核酸內(nèi)切酶SacⅡ和KpnⅠ的酶切位點(diǎn)欧美湿身诱惑超碰在线，分別對(duì)MDPV-Q和由該株病毒經(jīng)人工致弱后得到的MDPV-26株進(jìn)行PCR擴(kuò)增午夜毛片视频高清不卡免费，并將PCR產(chǎn)物克隆到pMD18-T載體上，分別得到二個(gè)重組子：pMD18-T—M-Q VP2和pMD18-T-M-26 VP2色哟哟精品入口。

It also indicated that the oospore production of Mtr isolates was obviously less than those of the parental isolates, and that the mycelium production of Mtr isolates was a little reduced compared to those of the parental isolates in liquid culture.

研究結(jié)果還表明a片黄这线免费观看1区，抗性菌株的卵孢子的產(chǎn)生量也明顯低于其親本菌株，抗性菌株在液體培養(yǎng)基中的菌絲較其親本菌株生長量有所減少国产绿帽精品一级视频。

12 Strains possessing higher peptidase activity were isolated from Chinese traditional fermented food.

試驗(yàn)從中國傳統(tǒng)發(fā)酵食品中分離出12株具有較高肽酶活力的菌株肏屄手机视频播放，經(jīng)進(jìn)一步篩選国产午夜一级福利毛片视频6，得到了兩株具有較好促進(jìn)干酪成熟能力的菌株，有望作為附屬發(fā)酵劑應(yīng)用于切達(dá)干酪生產(chǎn)日本视频www.色。

In order to evaluate the capability of lignin degradation and pitch elimination from Masson pine chips by nature white-rot fungi, 20 white-rot fungus strains were obtained by petri dishes screening and petri dishes color-reaction screening. One G strain with the lowest Mycel/Col value was then inoculated onto the surface of Masson pine chip, and the degradation rates of lignin and pitch were measured after 3, 5, 7, 9 days, respectively.

為評(píng)價(jià)從野外篩選得到的白腐菌對(duì)造紙?jiān)像R尾松的木素降解和樹脂脫除能力欧美XXXXX高潮在线观看，將帶菌樣本經(jīng)室內(nèi)平板初篩及平板變色反應(yīng)復(fù)篩，得到了20株純白腐菌菌株国产a片播放网址，并對(duì)變色系數(shù)最小的一株菌株G進(jìn)行松木片固體接種試驗(yàn)日韩AV操逼，測(cè)定3、5三级片手机免费看中文视频、7一区二区亚洲、9天后松木片的木素、樹脂降解情況欧美黄色午夜。

In order to evaluate the capability of lignin degradation and pitch elimination from Masson pine chips by nature white-rot fungi,20 white-rot fungus strains were obtained by petri dishes screening and petri dishes color-reaction screening.

為評(píng)價(jià)從野外篩選得到的白腐菌對(duì)造紙?jiān)像R尾松的木素降解和樹脂脫除能力欧美在线亚洲一区二区欧美，將帶菌樣本經(jīng)室內(nèi)平板初篩及平板變色反應(yīng)復(fù)篩，得到了20株純白腐菌菌株色色黄片，并對(duì)變色系數(shù)最小的一株菌株G進(jìn)行松木片固體接種試驗(yàn)久久精品9999热，測(cè)定3、5美女下面网站、7野狼网址、9天后松木片的木素、樹脂降解情況国产一国产一级毛片视频在线。

A tree whose hungry mouth is prest againsr the earth's sweet flowing breast; a tree that looks at god all day, and lifts her leafy arms to pry; a tree that may in summer wear a nest of robins in her hair; upon whose bosom snow has lain; who intimately lives with rain.

一株樹大学情侣酒店，他的饑渴的嘴吮吸著大地的甘露。一株樹野外免费视频毛片，他整日望著天高擎著葉臂黄页网在线观看视频大全，祈禱無語。一株樹国产一级av片精品，夏天在他的發(fā)間會(huì)有知更鳥砌巢居住最新永久福利地址发布页。一株樹，白雪躺在他胸上91视频蘑菇，他和雨是親密的伴侶免费一级欧美性大片口咬。